Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IU367_RS19655 Genome accession   NZ_CP064746
Coordinates   4240992..4242707 (+) Length   571 a.a.
NCBI ID   WP_274454404.1    Uniprot ID   -
Organism   Aeromonas bestiarum strain YK48     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4235992..4247707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IU367_RS19630 (IU367_19515) pdhR 4237143..4237907 (-) 765 WP_042039018.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  IU367_RS19635 (IU367_19520) ampD 4238184..4238756 (-) 573 WP_043554347.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IU367_RS19640 (IU367_19525) - 4238905..4239375 (+) 471 WP_181003660.1 retropepsin-like aspartic protease family protein -
  IU367_RS19645 (IU367_19530) nadC 4239381..4240238 (+) 858 WP_274452884.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IU367_RS19650 (IU367_19535) - 4240561..4241001 (+) 441 WP_274452886.1 pilin -
  IU367_RS19655 (IU367_19540) pilB 4240992..4242707 (+) 1716 WP_274454404.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  IU367_RS19660 (IU367_19545) pilC 4242840..4244081 (+) 1242 WP_098981882.1 type II secretion system F family protein Machinery gene
  IU367_RS19665 (IU367_19550) pilD 4244144..4245016 (+) 873 WP_274452887.1 type IVa prepilin peptidase/methyltransferase TapD Machinery gene
  IU367_RS19670 (IU367_19555) coaE 4245035..4245649 (+) 615 WP_103472090.1 dephospho-CoA kinase -
  IU367_RS19675 (IU367_19560) zapD 4245687..4246409 (+) 723 WP_043139926.1 cell division protein ZapD -
  IU367_RS19680 (IU367_19565) yacG 4246419..4246613 (+) 195 WP_042039030.1 DNA gyrase inhibitor YacG -
  IU367_RS19685 (IU367_19570) mutT 4246687..4247094 (-) 408 WP_241279560.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62894.40 Da        Isoelectric Point: 5.4321

>NTDB_id=504512 IU367_RS19655 WP_274454404.1 4240992..4242707(+) (pilB) [Aeromonas bestiarum strain YK48]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLLESDQDALGMEDIDE
SEISELEVSDENSRLDESVNTTDDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAAEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKDCSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=504512 IU367_RS19655 WP_274454404.1 4240992..4242707(+) (pilB) [Aeromonas bestiarum strain YK48]
CTGCCCATAATGACATCTAGCCCCAATAGCGGTCTGGCGTTAAGCCTGGCCGCTTCTTCCCTGCTTAGTGAGTCCGACTC
ACAGCGCTACCTGAGCCAAGCCAAGGCCCAGCGCAAGCCCTTTGTGACCTTTCTGATAGAGAACGAGATCCTCGACAGCA
AGGCGCTGGCGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAATACCTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTATACCCAGGGCCATACCCTCTATATCGC
CATGTCGGATCCGACCAACGTCTCGGCGCTGGAGGATTTCGGCTTCAGCTTCGGGCTGCATACCGAGGCGCTGCTGGTCG
AAGAGAACAAGCTGACGACCGCCATCGGCAAGCTGTTGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAG
TCCGAGATCTCTGAGCTGGAAGTCTCGGACGAAAACTCACGACTCGATGAGAGCGTCAACACCACCGACGACGACGCCCC
CATCGTAAAATACATCAATAAAATCATGATGGATGCCATCAAACGTGGCGCATCCGATCTGCACTTCGAGCCCTATGAGA
CCAAATACCGCATCCGCTTTCGGATCGACGGCATATTGCACGAAATCGCGACGCCACCGGTCAATCTGGCCAACCGCTTC
TCCGCCCGCCTCAAGGTCATGGCACGGCTCGACATCGCAGAGCGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAACT
GTCCCGCAACAAGTCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGATAGTCATCCGCTTGCTGG
ACTCCTCGGCGGCGCGGCTCAACATAGAGCAACTCGGCTTCGATGACCGGCAGAAGGCCCAATATCTGCGTGCCCTCTCC
AAGCCGCAGGGCATGATCTTGGTGACAGGCCCTACCGGCTCGGGTAAGACAGTCTCCCTCTATACCGGTCTCAATATTCT
CAACACCACGGAGGTGAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCAGGCGTCAATCAGGTACAGGTCA
ACCCCAAGGCAGGGCTTACCTTCGCCAGTGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGACGTGGTCATGGTGGGGGAG
ATCCGGGATCTGGAAACCGCCGAGATCGCCATCAAGGCCGCTCAGACAGGCCACTTGGTGCTCTCCACCCTGCACACCAA
CTCGGCCGCCGAAACCCTGACCCGCATGATGAACATGGGAGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCA
TGGCCCAGCGACTCGCCCGCAAGCTGTGCGATCATTGCAAGGCCGCCGAGGTGGTACCCGAAGCCGAACTGCTGGAGCTG
GGATTCACCCAGCAGCAGCTGGCAGCTGGACTACGCCTGTTCAAACCCGTGGGGTGCAAGGATTGCTCGGGTGGCTACAA
AGGGCGGGTCGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGTGCCAACTCTCTGC
AAATTGCGGCCATTGCTCAAAAAGAGGGGATGCGCACTCTGCGTATATCAGGCCTTGAGAAGGCTCGTCTGGGAGTGACC
AGCCTGGCAGAAATCAATCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

54.625

100

0.548
  pilB Legionella pneumophila strain ERS1305867

54.69

98.949

0.541
  pilB Vibrio cholerae strain A1552

54.159

98.949

0.536
  pilB Vibrio campbellii strain DS40M4

50.871

100

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.424

0.501
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

96.848

0.378
  pilF Thermus thermophilus HB27

40.152

92.469

0.371