Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IVG45_RS19465 Genome accession   NZ_CP064653
Coordinates   4154947..4156656 (+) Length   569 a.a.
NCBI ID   WP_196435421.1    Uniprot ID   A0A7T0NXS1
Organism   Methylomonas sp. LL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4149947..4161656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IVG45_RS19455 (IVG45_19410) - 4152593..4154023 (+) 1431 WP_230874661.1 HDOD domain-containing protein -
  IVG45_RS19460 (IVG45_19415) - 4154026..4154817 (+) 792 WP_230874662.1 response regulator -
  IVG45_RS19465 (IVG45_19420) pilB 4154947..4156656 (+) 1710 WP_196435421.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IVG45_RS19470 (IVG45_19425) pilC 4156660..4157877 (+) 1218 WP_196435422.1 type II secretion system F family protein Machinery gene
  IVG45_RS19475 (IVG45_19430) pilD 4157956..4158819 (+) 864 WP_196435423.1 A24 family peptidase Machinery gene
  IVG45_RS19480 (IVG45_19435) coaE 4158823..4159434 (+) 612 WP_196435424.1 dephospho-CoA kinase -
  IVG45_RS19485 (IVG45_19440) zapD 4159431..4160201 (+) 771 WP_196435425.1 cell division protein ZapD -
  IVG45_RS19490 (IVG45_19445) yacG 4160201..4160404 (+) 204 WP_196435426.1 DNA gyrase inhibitor YacG -
  IVG45_RS19495 (IVG45_19450) - 4160419..4161351 (-) 933 WP_196435427.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62798.34 Da        Isoelectric Point: 5.4734

>NTDB_id=504055 IVG45_RS19465 WP_196435421.1 4154947..4156656(+) (pilB) [Methylomonas sp. LL1]
MATAPSNIQFSGLAKCLIQQGLLTEESAATAVQEAQKNKQPFVSYLVKNKFVDSRTVAGLASAEFGVPLLDLDAIDLSVI
PIRLVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRLNTENILVEEDKLIKTIETALEAVDATISNLLDE
DLDNIDISSGDERPVEESTSDVDDAPIVRFVNKILLDSIKKGVSDIHLEPFEKKFRIRFRADGILSEIASPPVGIANRIT
SRIKVMSKMDIAERRVPQDGRIKMQLSKNRAIDFRVNTCPTLFGEKVVMRILDPTSAQIGIEKLGFEPVQQQQFLSAINK
PYGMVLVTGPTGSGKTVSLYTGLNLLNSVERNISTAEDPVEITVEGINQVNVNPKAGLTFAEALRAFLRQDPDVIMVGEI
RDLETASIAVKAAQTGHLVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVLLIMAQRLARRLCEYCKKEADFPDNILLSAG
FKQEDIGSFKVYEPVGCEHCNNGYKGRVGIYQVLPITEKMRALILRGGNAMEMAELAKEEGINDLRASGLLKVRQGITSL
EEIDRVTKE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=504055 IVG45_RS19465 WP_196435421.1 4154947..4156656(+) (pilB) [Methylomonas sp. LL1]
ATGGCGACAGCACCTTCAAATATTCAGTTTAGCGGTCTGGCTAAATGCCTTATTCAGCAAGGCTTGCTTACAGAGGAATC
CGCCGCAACAGCCGTGCAGGAAGCGCAAAAAAATAAACAACCCTTCGTTTCCTATCTGGTCAAAAATAAATTTGTCGACA
GCCGCACGGTTGCGGGCTTGGCGTCGGCGGAATTTGGCGTGCCGCTGTTGGATTTGGATGCTATCGATTTAAGCGTGATA
CCGATCAGGTTGGTCAGCGAAAAACTGATACGCCAGCATCATGCCTTACCCTTATTCAAGCGCGGCAATCGGTTGTTTGT
CGCCACTGCCGACCCGACTAATTTCCAGGCCTTGGATGAAATAAAATTTCATACCCGGTTGAATACCGAAAATATTCTGG
TCGAAGAAGATAAGCTGATCAAAACCATAGAAACGGCGTTGGAAGCGGTTGATGCGACCATCTCGAATCTGCTGGATGAA
GATCTGGATAATATCGATATTTCCAGTGGCGATGAAAGACCGGTCGAAGAATCGACTTCCGATGTCGACGATGCGCCGAT
TGTGCGGTTCGTTAACAAGATATTGTTGGATTCGATCAAAAAGGGCGTTTCGGACATACATTTGGAGCCTTTCGAAAAAA
AATTCCGTATACGCTTTCGGGCCGACGGTATCTTGTCCGAAATTGCCAGCCCCCCGGTCGGCATTGCCAATCGGATTACT
TCCAGGATCAAGGTCATGTCTAAAATGGACATCGCCGAACGGCGCGTGCCTCAGGACGGCCGGATTAAGATGCAGCTTTC
CAAAAATCGCGCAATCGATTTTCGGGTCAACACCTGTCCGACTTTGTTCGGCGAGAAAGTGGTGATGCGGATACTCGATC
CCACCAGTGCCCAGATAGGCATTGAAAAATTGGGTTTCGAGCCGGTTCAACAACAACAATTTTTATCGGCTATCAACAAA
CCCTATGGCATGGTGTTGGTGACCGGTCCTACCGGTAGCGGTAAGACGGTGTCGCTGTATACCGGCCTGAATCTTTTGAA
CAGCGTTGAGCGCAATATATCCACCGCCGAGGATCCGGTGGAAATCACGGTGGAGGGCATCAATCAGGTCAACGTCAATC
CCAAGGCCGGTTTGACTTTTGCCGAAGCCTTGCGGGCATTTTTACGGCAAGACCCGGATGTGATCATGGTTGGCGAGATT
CGCGACCTGGAAACCGCCAGCATTGCCGTCAAGGCCGCGCAAACCGGACACTTAGTGTTGTCAACCCTGCATACCAACGA
TGCGCCGCAAACCATTAACCGCTTGGTGCAGATGGGTATTGAGCCGTTCAATATCGTATCCGCGGTGCTGTTGATCATGG
CCCAACGCTTGGCGCGGCGCTTATGTGAATATTGTAAAAAAGAAGCCGATTTTCCGGACAATATACTGTTGTCGGCCGGA
TTCAAACAGGAAGATATCGGCAGCTTCAAAGTTTACGAGCCGGTGGGTTGCGAACATTGCAATAATGGCTATAAAGGCCG
GGTAGGTATTTACCAAGTATTGCCCATCACTGAAAAAATGCGGGCGTTGATTTTACGCGGCGGTAATGCAATGGAAATGG
CAGAGTTAGCCAAGGAAGAAGGCATCAACGACCTGAGAGCATCCGGCTTGCTAAAAGTCCGCCAGGGTATTACCTCCCTG
GAAGAAATCGATCGAGTTACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-144)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T0NXS1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.294

100

0.566
  pilB Acinetobacter baumannii D1279779

53.671

100

0.54
  pilB Acinetobacter baylyi ADP1

54.078

99.121

0.536
  pilB Vibrio parahaemolyticus RIMD 2210633

47.059

98.594

0.464
  pilF Neisseria gonorrhoeae MS11

47.302

97.715

0.462
  pilB Vibrio cholerae strain A1552

51.081

89.455

0.457
  pilB Vibrio campbellii strain DS40M4

45.567

99.121

0.452
  pilF Thermus thermophilus HB27

38.998

98.243

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

39.526

96.485

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.478

100

0.376