Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IUJ49_RS20865 Genome accession   NZ_CP064382
Coordinates   4516890..4518596 (+) Length   568 a.a.
NCBI ID   WP_045525681.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain PartN-Ahydrophila-RM8376     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4511890..4523596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ49_RS20840 (IUJ49_20830) pdhR 4513039..4513803 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  IUJ49_RS20845 (IUJ49_20835) ampD 4514079..4514651 (-) 573 WP_045525676.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IUJ49_RS20850 (IUJ49_20840) - 4514799..4515269 (+) 471 WP_024944772.1 retropepsin-like aspartic protease -
  IUJ49_RS20855 (IUJ49_20845) nadC 4515275..4516132 (+) 858 WP_045525678.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IUJ49_RS20860 (IUJ49_20850) - 4516455..4516886 (+) 432 WP_106551646.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IUJ49_RS20865 (IUJ49_20855) pilB 4516890..4518596 (+) 1707 WP_045525681.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  IUJ49_RS20870 (IUJ49_20860) pilC 4518712..4519953 (+) 1242 WP_045525683.1 type II secretion system F family protein Machinery gene
  IUJ49_RS20875 (IUJ49_20865) pilD 4519976..4520848 (+) 873 WP_045525685.1 A24 family peptidase Machinery gene
  IUJ49_RS20880 (IUJ49_20870) coaE 4520868..4521482 (+) 615 WP_045525686.1 dephospho-CoA kinase -
  IUJ49_RS20885 (IUJ49_20875) zapD 4521520..4522242 (+) 723 WP_024945431.1 cell division protein ZapD -
  IUJ49_RS20890 (IUJ49_20880) yacG 4522252..4522446 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  IUJ49_RS20895 (IUJ49_20885) mutT 4522507..4522920 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62497.87 Da        Isoelectric Point: 5.4342

>NTDB_id=501489 IUJ49_RS20865 WP_045525681.1 4516890..4518596(+) (pilB) [Aeromonas hydrophila strain PartN-Ahydrophila-RM8376]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=501489 IUJ49_RS20865 WP_045525681.1 4516890..4518596(+) (pilB) [Aeromonas hydrophila strain PartN-Ahydrophila-RM8376]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
CCTGAGTCAAGCCAAGGCCCAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTAGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTCTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTAGAAGAGAACA
AACTCACCACTGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAATTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGACAGAAGGCGCAATATCTGCGTGCTCTGTCGAAACCTCAG
GGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCTGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGTGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACTCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTAGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAACTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTGGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCTGGCCTTGAGAAGGCTCGCCTGGGTGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.767

99.648

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373