Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IS519_RS13270 Genome accession   NZ_CP064170
Coordinates   2895455..2897140 (-) Length   561 a.a.
NCBI ID   WP_248639141.1    Uniprot ID   -
Organism   Vibrio crassostreae strain ED395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2890455..2902140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS519_RS13240 (IS519_13230) rplS 2890942..2891295 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  IS519_RS13245 (IS519_13235) yacG 2891612..2891809 (-) 198 WP_019823814.1 DNA gyrase inhibitor YacG -
  IS519_RS13250 (IS519_13240) zapD 2891891..2892631 (-) 741 WP_017063237.1 cell division protein ZapD -
  IS519_RS13255 (IS519_13245) coaE 2892658..2893272 (-) 615 WP_248639138.1 dephospho-CoA kinase -
  IS519_RS13260 (IS519_13250) pilD 2893275..2894144 (-) 870 WP_017058945.1 A24 family peptidase Machinery gene
  IS519_RS13265 (IS519_13255) pilC 2894210..2895439 (-) 1230 WP_048612336.1 type II secretion system F family protein Machinery gene
  IS519_RS13270 (IS519_13260) pilB 2895455..2897140 (-) 1686 WP_248639141.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IS519_RS13275 (IS519_13265) pilA 2897140..2897610 (-) 471 WP_248639143.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  IS519_RS13280 (IS519_13270) nadC 2897864..2898754 (-) 891 WP_248639145.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IS519_RS13285 (IS519_13275) ampD 2898838..2899437 (+) 600 WP_248639148.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IS519_RS13290 (IS519_13280) pdhR 2899921..2900682 (+) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62291.94 Da        Isoelectric Point: 4.9878

>NTDB_id=499954 IS519_RS13270 WP_248639141.1 2895455..2897140(-) (pilB) [Vibrio crassostreae strain ED395]
MLTNLPTVLRQADLLSLTQEQAVAEHIHASGVSTPEALLFLELFSGDSLANNIKTIFGLPLVQLAKTDYETLCDQLGLRE
LITKYRAIPISVSNSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEIASIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDDLAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKETDDSPDIFLRHSIPNNQT
IYKANPQGCNECNQGYSGRVGIYEVMPFSDQLKNSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=499954 IS519_RS13270 WP_248639141.1 2895455..2897140(-) (pilB) [Vibrio crassostreae strain ED395]
ATGCTAACCAACCTCCCAACCGTTCTTCGTCAGGCTGACTTACTTAGCCTGACTCAAGAACAAGCCGTTGCGGAACATAT
ACATGCTTCAGGTGTGTCTACACCTGAAGCTTTGCTCTTTCTGGAGCTCTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACAACTTGCCAAGACTGACTATGAGACCTTATGTGACCAATTAGGGCTTCGTGAA
CTGATAACCAAGTATCGTGCGATACCAATTTCAGTCTCTAACTCTACTCTCACGCTCGCTTCTGCAGACCCGACCGATTT
ACAAGCTGAAGATGATTTCCGCTTTGCGACCGGATTACAGATTGAATTGGTTGTCGCCAACTACTCAGAACTAGAAGGCG
CTATACGCAAGCTGTATGGCCGTTCTATTTCAGGGCAAGATTCCAAGCGAAAAGAGATCACTCAAGATGAACTCGCCAAT
CTTGTTGAGGTGTCTGACGACGAGATAGCGTCGATAGAAGATCTCAGTCAAGACGATTCCCCTGTCAGCAGATTTATCAA
TCAGATATTGGTTGATGCGGTGCGTAAAGGTGCATCAGATATCCACTTCGAACCTTATGAAGAACACTACCGAGTACGCC
TGCGTTGTGATGGCATCCTTGTCGAGATCCAACAACCCGCCTCTCACTTAAGTCGTCGTCTATCAGCTCGTTTAAAGATT
CTCGCCAAGCTTGATATCGCTGAACGTCGCCTGCCTCAAGACGGTCGTATTAAACTGCGTCTAAATGACGACTTGGCGAT
AGATATGCGAGTCTCCACATTACCAACCTTGTGGGGTGAGAAAATTGTACTGAGGCTACTCGATAGCAGCGCTGCTAATT
TAGATATCGATAAGTTAGGTTATAACGAAGATCAAAAAGCACTCTACCTTAATGCATTGAAACGCCCACAAGGGATGATC
TTAATGACCGGGCCCACAGGCAGCGGAAAAACCGTCTCCCTTTATACTGGCCTTCGAGTGCTCAACACTACTGAACGCAA
CATCTCAACAGCTGAAGACCCAGTGGAGATTAACCTGTGTGGTATCAATCAAGTACAAGTAACACCTAAGATCGGCTTTG
GGTTTGCTGAAGCACTTCGATCATTTTTGCGACAAGATCCTGATGTTGTAATGGTTGGAGAGATCCGCGACTTGGAAACA
GCAGAGATCGCGATCAAAGCATCGCAAACGGGTCACTTGGTCCTTTCAACACTGCACACGAACTCCGCCGCAGAAACCGT
CACTCGACTCGCTCATATGGGGATAGAGCCCTTTAACCTCGCTTCATCATTGAGTTTGATTATCGCCCAGCGACTGGCAA
GAAGATTATGCAACCACTGCAAAGAAACTGACGATTCACCAGACATATTTCTGCGTCACTCGATTCCTAACAACCAAACC
ATCTACAAAGCGAATCCTCAAGGGTGCAATGAGTGTAACCAGGGGTATTCAGGTCGAGTGGGTATCTATGAAGTAATGCC
GTTTAGCGATCAACTGAAAAATAGCCTGATAGACAAACCTAACGCGTTAGCCATTGAAAACTTGGCACGTCGAGAAGGCA
TGAGAACTCTGCAAGAGTCGGGGCTAGATAAATTGCTTGAAGGCACCACCAGCTATCAAGAGCTGCAACGTGTTCTGTAC
ATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.712

100

0.74
  pilB Vibrio campbellii strain DS40M4

70.107

100

0.702
  pilB Vibrio parahaemolyticus RIMD 2210633

70.107

100

0.702
  pilB Acinetobacter baumannii D1279779

50.775

91.979

0.467
  pilB Legionella pneumophila strain ERS1305867

49.524

93.583

0.463
  pilB Acinetobacter baylyi ADP1

49.133

92.513

0.455
  pilF Neisseria gonorrhoeae MS11

43.784

98.93

0.433
  pilF Thermus thermophilus HB27

38.772

92.87

0.36