Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IS519_RS13275 Genome accession   NZ_CP064170
Coordinates   2897140..2897610 (-) Length   156 a.a.
NCBI ID   WP_248639143.1    Uniprot ID   -
Organism   Vibrio crassostreae strain ED395     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2892140..2902610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS519_RS13255 (IS519_13245) coaE 2892658..2893272 (-) 615 WP_248639138.1 dephospho-CoA kinase -
  IS519_RS13260 (IS519_13250) pilD 2893275..2894144 (-) 870 WP_017058945.1 A24 family peptidase Machinery gene
  IS519_RS13265 (IS519_13255) pilC 2894210..2895439 (-) 1230 WP_048612336.1 type II secretion system F family protein Machinery gene
  IS519_RS13270 (IS519_13260) pilB 2895455..2897140 (-) 1686 WP_248639141.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IS519_RS13275 (IS519_13265) pilA 2897140..2897610 (-) 471 WP_248639143.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  IS519_RS13280 (IS519_13270) nadC 2897864..2898754 (-) 891 WP_248639145.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IS519_RS13285 (IS519_13275) ampD 2898838..2899437 (+) 600 WP_248639148.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IS519_RS13290 (IS519_13280) pdhR 2899921..2900682 (+) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16447.76 Da        Isoelectric Point: 4.8354

>NTDB_id=499955 IS519_RS13275 WP_248639143.1 2897140..2897610(-) (pilA) [Vibrio crassostreae strain ED395]
MKNKRTNQKGFTLIELMIVVAIIGILSAFAVPAYQDYTKKATLAEFPKAAAAVKLAVELCAHENGQANFASSCISSTSSA
ATTVPAELILNEIKIYAQAGTLTSGAVEVIAEANSSKGPIAATEKYVMAADFQNEGIVWTNTCFDTNDASQTAYCP

Nucleotide


Download         Length: 471 bp        

>NTDB_id=499955 IS519_RS13275 WP_248639143.1 2897140..2897610(-) (pilA) [Vibrio crassostreae strain ED395]
ATGAAGAACAAAAGAACGAACCAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTATTGGCATTTTGTC
GGCTTTTGCGGTGCCTGCTTATCAAGACTATACAAAAAAGGCTACTCTGGCTGAGTTTCCTAAAGCAGCTGCGGCTGTCA
AGTTAGCAGTTGAGCTTTGTGCTCACGAAAATGGTCAAGCCAACTTTGCAAGTAGTTGTATAAGCTCAACGTCATCAGCT
GCAACAACAGTTCCTGCAGAGTTAATCCTAAACGAGATTAAGATCTATGCTCAGGCAGGTACCCTAACGTCTGGTGCGGT
AGAAGTCATAGCTGAAGCTAACAGCTCTAAAGGTCCAATTGCTGCAACAGAGAAATATGTTATGGCTGCAGATTTTCAAA
ATGAAGGTATTGTTTGGACCAATACTTGTTTTGACACTAACGATGCTTCACAAACAGCATACTGCCCATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

50

100

0.5