Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ISN32_RS03785 Genome accession   NZ_CP064003
Coordinates   912032..913162 (+) Length   376 a.a.
NCBI ID   WP_003478080.1    Uniprot ID   A0A1C3TLF5
Organism   Xanthomonas translucens pv. translucens strain XtKm9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 907032..918162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN32_RS03760 (ISN32_03760) - 907060..907515 (-) 456 WP_058359887.1 DUF4426 domain-containing protein -
  ISN32_RS03765 (ISN32_03765) proC 907530..908372 (-) 843 WP_058359888.1 pyrroline-5-carboxylate reductase -
  ISN32_RS03770 (ISN32_03770) - 908502..909551 (-) 1050 WP_193565946.1 hypothetical protein -
  ISN32_RS03775 (ISN32_03775) - 910071..910721 (-) 651 WP_058359937.1 YggS family pyridoxal phosphate-dependent enzyme -
  ISN32_RS03780 (ISN32_03780) pilT 910846..911883 (+) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  ISN32_RS03785 (ISN32_03785) pilU 912032..913162 (+) 1131 WP_003478080.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ISN32_RS03790 (ISN32_03790) - 913418..913957 (-) 540 WP_003471664.1 DNA-3-methyladenine glycosylase I -
  ISN32_RS03795 (ISN32_03795) - 913973..915781 (-) 1809 WP_058359938.1 DUF4153 domain-containing protein -
  ISN32_RS03800 (ISN32_03800) - 915889..916455 (+) 567 WP_003471660.1 YqgE/AlgH family protein -
  ISN32_RS03805 (ISN32_03805) ruvX 916448..916945 (+) 498 WP_058359890.1 Holliday junction resolvase RuvX -
  ISN32_RS03810 (ISN32_03810) - 916965..917912 (+) 948 WP_058359891.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41956.32 Da        Isoelectric Point: 6.8811

>NTDB_id=498475 ISN32_RS03785 WP_003478080.1 912032..913162(+) (pilU) [Xanthomonas translucens pv. translucens strain XtKm9]
MSIIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPSPDGKSRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=498475 ISN32_RS03785 WP_003478080.1 912032..913162(+) (pilU) [Xanthomonas translucens pv. translucens strain XtKm9]
ATGAGCATCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
CACCGTCGATGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCAATCTCCTTCGCCGAGACCGGCCATCTGGTGCTGTGCACCCTGCATGCCAACAACGCCAATCAGGCGATG
GACCGGATCATCAATTTCTTTCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAATATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGAGCCCCGACGGCAAGAGCCGCCGCGTGGCGATGGAGATCATGCTCGGTACGCCGCTGGTGC
AGGACTACATCCGCGAAGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGTGTGGAAATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3TLF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.312

98.138

0.641
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367