Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ISN33_RS08670 Genome accession   NZ_CP064002
Coordinates   2033777..2034907 (+) Length   376 a.a.
NCBI ID   WP_003471665.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. translucens strain XtKm33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2028777..2039907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN33_RS08645 (ISN33_08630) - 2029015..2029470 (-) 456 WP_003471670.1 DUF4426 domain-containing protein -
  ISN33_RS08650 (ISN33_08635) proC 2029485..2030333 (-) 849 WP_038239378.1 pyrroline-5-carboxylate reductase -
  ISN33_RS08655 (ISN33_08640) - 2030476..2031408 (-) 933 WP_230811868.1 hypothetical protein -
  ISN33_RS08660 (ISN33_08645) - 2031816..2032466 (-) 651 WP_068873425.1 YggS family pyridoxal phosphate-dependent enzyme -
  ISN33_RS08665 (ISN33_08650) pilT 2032591..2033628 (+) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  ISN33_RS08670 (ISN33_08655) pilU 2033777..2034907 (+) 1131 WP_003471665.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ISN33_RS08675 (ISN33_08660) - 2035163..2035702 (-) 540 WP_003471664.1 DNA-3-methyladenine glycosylase I -
  ISN33_RS08680 (ISN33_08665) - 2035718..2037526 (-) 1809 WP_003471662.1 DUF4153 domain-containing protein -
  ISN33_RS08685 (ISN33_08670) - 2037634..2038200 (+) 567 WP_003471660.1 YqgE/AlgH family protein -
  ISN33_RS08690 (ISN33_08675) ruvX 2038193..2038690 (+) 498 WP_047325118.1 Holliday junction resolvase RuvX -
  ISN33_RS08695 (ISN33_08680) - 2038710..2039657 (+) 948 WP_003471658.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41916.22 Da        Isoelectric Point: 6.8810

>NTDB_id=498461 ISN33_RS08670 WP_003471665.1 2033777..2034907(+) (pilU) [Xanthomonas translucens pv. translucens strain XtKm33]
MSTIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNMRGVVAQQLIPSPDGKSRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYDDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=498461 ISN33_RS08670 WP_003471665.1 2033777..2034907(+) (pilU) [Xanthomonas translucens pv. translucens strain XtKm33]
ATGAGCACCATCGACTTCACCTCATTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGTTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
CACCGTCGACGAACTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCATGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAGACCGGCCATCTGGTGCTGTGCACCCTGCATGCCAACAACGCCAATCAGGCGATG
GACCGGGTCATCAATTTCTTTCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAATATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGAGCCCCGACGGCAAGAGCCGCCGCGTGGCGATGGAGATCATGCTCGGTACGCCGCTGGTGC
AGGACTACATCCGCGAAGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGATGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGTGTGGAAATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.77

98.138

0.636
  pilU Acinetobacter baylyi ADP1

63.305

94.947

0.601
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367