Detailed information NTG0049770

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SSUD12_RS00795
• Genome accession: NC_017621
• Coordinates: 128047..128481 (+)
• Length: 144 a.a.
• NCBI ID: WP_014637316.1
• Uniprot ID: G7SD64
• Organism: Streptococcus suis D12
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 123047..133481

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SSUD12_RS00760 (SSUD12_0121)	-	123789..124154 (+)	366	WP_014637310.1	DUF1033 family protein	-
SSUD12_RS00765 (SSUD12_0122)	-	124189..125139 (-)	951	WP_014637311.1	S66 peptidase family protein	-
SSUD12_RS00770 (SSUD12_0123)	comYA	125225..126175 (+)	951	WP_014637312.1	competence type IV pilus ATPase ComGA	Machinery gene
SSUD12_RS00775 (SSUD12_0124)	comYB	126087..127124 (+)	1038	WP_044669087.1	competence type IV pilus assembly protein ComGB	Machinery gene
SSUD12_RS00780 (SSUD12_0125)	comYC	127126..127407 (+)	282	WP_002936550.1	competence type IV pilus major pilin ComGC	Machinery gene
SSUD12_RS00785 (SSUD12_0126)	comGD	127388..127795 (+)	408	WP_014637314.1	competence type IV pilus minor pilin ComGD	-
SSUD12_RS00790 (SSUD12_0127)	comYE	127767..128060 (+)	294	WP_024410167.1	competence type IV pilus minor pilin ComGE	Machinery gene
SSUD12_RS00795 (SSUD12_0128)	comGF/cglF	128047..128481 (+)	435	WP_014637316.1	competence type IV pilus minor pilin ComGF	Machinery gene
SSUD12_RS00800 (SSUD12_0129)	comGG	128459..128869 (+)	411	WP_014637317.1	competence type IV pilus minor pilin ComGG	-
SSUD12_RS00805 (SSUD12_0130)	comYH	128926..129879 (+)	954	WP_014637318.1	class I SAM-dependent methyltransferase	Machinery gene
SSUD12_RS00810 (SSUD12_0131)	-	129929..131116 (+)	1188	WP_014637319.1	acetate kinase	-
SSUD12_RS00815 (SSUD12_0132)	-	131377..131928 (+)	552	WP_014637320.1	folate family ECF transporter S component	-
SSUD12_RS00820 (SSUD12_0133)	-	131984..133240 (+)	1257	WP_014637321.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16405.75 Da
• Isoelectric Point: 6.9830

>NTDB_id=49770 SSUD12_RS00795 WP_014637316.1 128047..128481(+) (comGF/cglF) [Streptococcus suis D12]
MLKSKLPAFTLLECLVALIILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSRSDDFRKTNDKGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide

• Length: 435 bp

>NTDB_id=49770 SSUD12_RS00795 WP_014637316.1 128047..128481(+) (comGF/cglF) [Streptococcus suis D12]
TTGTTAAAAAGTAAACTTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAAGGGACAGGGTTACCAGCCCATGCT
CTATCAGGTGGACAGCGCAGCCATTTCCCAAGAAAACCAGCTAGTGCGCATCGACTTTAGCTTTAAAAATGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

3D structure

• AlphaFold DB: G7SD64

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae D39	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae R6	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae TIGR4	53.191	97.917	0.521
comYF	Streptococcus mutans UA140	50.694	100	0.507
comYF	Streptococcus mutans UA159	50	100	0.5
comGF/cglF	Streptococcus mitis NCTC 12261	53.731	93.056	0.5
comGF/cglF	Streptococcus mitis SK321	51.493	93.056	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	46.809	97.917	0.458