Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IOD65_RS18005 Genome accession   NZ_CP063769
Coordinates   3779680..3781392 (+) Length   570 a.a.
NCBI ID   WP_005065876.1    Uniprot ID   -
Organism   Acinetobacter sp. Ac-14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3774680..3786392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IOD65_RS17970 (IOD65_17970) nusA 3775568..3777052 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  IOD65_RS17975 (IOD65_17975) rimP 3777090..3777614 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  IOD65_RS17995 (IOD65_17995) secG 3778252..3778581 (-) 330 WP_005065880.1 preprotein translocase subunit SecG -
  IOD65_RS18000 (IOD65_18000) tpiA 3778594..3779388 (-) 795 WP_005065878.1 triose-phosphate isomerase -
  IOD65_RS18005 (IOD65_18005) pilB 3779680..3781392 (+) 1713 WP_005065876.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IOD65_RS18010 (IOD65_18010) pilC 3781419..3782645 (+) 1227 WP_005065874.1 type II secretion system F family protein Machinery gene
  IOD65_RS18015 (IOD65_18015) pilD 3782645..3783505 (+) 861 WP_033849614.1 prepilin peptidase Machinery gene
  IOD65_RS18020 (IOD65_18020) coaE 3783507..3784103 (+) 597 WP_005065869.1 dephospho-CoA kinase -
  IOD65_RS18025 (IOD65_18025) - 3784100..3785014 (-) 915 WP_057062693.1 DMT family transporter -
  IOD65_RS18030 (IOD65_18030) rlmB 3785050..3785799 (-) 750 WP_005065863.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IOD65_RS18035 (IOD65_18035) - 3785902..3786228 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63579.02 Da        Isoelectric Point: 5.0932

>NTDB_id=496679 IOD65_RS18005 WP_005065876.1 3779680..3781392(+) (pilB) [Acinetobacter sp. Ac-14]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLIEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=496679 IOD65_RS18005 WP_005065876.1 3779680..3781392(+) (pilB) [Acinetobacter sp. Ac-14]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTTTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGACCAGAAACTTATTTTAAAACATCGGGTCTTACCTCTTATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAACCCGAGTAATATTGAAGCTATTGATGCCATTCGCTTCAATAGCAAGCTCATGGTTGAGCCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCACAGCAATTTACCGAAGAAAGTAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACCCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTACGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAACCGTTTAGCTTCT
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCCATCGATTTTCGTGTGAACTCACTTCCAACTTTGTTTGGTGAAAAATTGGTTCTGCGTATTCTAGATCCAT
CTAGCGCAATGCTCGGCATTGATGCTCTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTTGATAAACCA
CAAGGTATGCTTCTCATTACAGGCCCTACAGGTTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAGGTTAACGTTAACCCGA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCTTTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAGATTGCGATTAAGGCTGCACAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCGACGTCTGTCAATCTGGTGATTGCGC
AACGTTTGGCGCGTCGGTTATGCCCGCAGTGTAAAATTCCTGCCGACATCCCTAAACAGAGCCTAATAGAAATGGGGTTT
ACCGAACAGGATTTAACCCACCCCGATTTTCAAGTTTTTCAACCAGTCGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGCGTCACTTCC
TTACAAGAAGTCAACCGCGTGACTAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.263

100

0.953
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.852

99.474

0.516
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

92.982

0.479
  pilB Vibrio campbellii strain DS40M4

50.958

91.579

0.467
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

36.655

98.596

0.361