Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INQ41_RS10380 Genome accession   NZ_CP063656
Coordinates   2302816..2304546 (+) Length   576 a.a.
NCBI ID   WP_193984253.1    Uniprot ID   A0A7S6UEZ2
Organism   Novilysobacter ciconiae strain H21R20     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2297816..2309546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ41_RS10360 (INQ41_10360) - 2298073..2299134 (-) 1062 WP_193984245.1 homoserine O-succinyltransferase -
  INQ41_RS10365 (INQ41_10365) - 2299450..2300586 (+) 1137 WP_193984247.1 phytase -
  INQ41_RS10370 (INQ41_10370) - 2300568..2301503 (+) 936 WP_193984249.1 M23 family metallopeptidase -
  INQ41_RS10375 (INQ41_10375) comP 2301873..2302265 (+) 393 WP_193984251.1 pilin Machinery gene
  INQ41_RS10380 (INQ41_10380) pilB 2302816..2304546 (+) 1731 WP_193984253.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INQ41_RS10385 (INQ41_10385) pilC 2304741..2306000 (+) 1260 WP_193984254.1 type II secretion system F family protein Machinery gene
  INQ41_RS10390 (INQ41_10390) pilD 2306014..2306877 (+) 864 WP_193984256.1 A24 family peptidase Machinery gene
  INQ41_RS10395 (INQ41_10395) coaE 2306960..2307580 (+) 621 WP_193987329.1 dephospho-CoA kinase -
  INQ41_RS10400 (INQ41_10400) - 2307702..2308694 (-) 993 WP_193987330.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62911.28 Da        Isoelectric Point: 5.7599

>NTDB_id=496130 INQ41_RS10380 WP_193984253.1 2302816..2304546(+) (pilB) [Novilysobacter ciconiae strain H21R20]
MNAVATANLVGITGIARRLVQDGALSESAAREAMAAATEQRKPLASYIIEKRLVAPAHLAAANSVEFGVPIFDAAAMDPE
QSAIKLVKEELLRKHTVLPLFKRGNRLFVGIAEPTNTHALAEIKFQTNFTVEAILVDEESIKRHLDRWLESADALGDVMG
EDDEGLENLDIGGGEDDLSADSGVDAKTDDTPVVKFINKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQSAKVPIK
LQPRISARLKVMAQLDIAERRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIEKLGYEDDQRDIF
LAAVKRPYGMVLVTGPTGSGKTVSLYTALNILNDETRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVSLVIAQRLARRLHSCKQKIELPEHA
LLAEGFTAEEIAAEDFTIYEAVGCADCTEGYKGRTGIYQVMPMTDEIQGIVLAGGNAMQIAEAAQKSGVRDLRQSALMKV
RNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=496130 INQ41_RS10380 WP_193984253.1 2302816..2304546(+) (pilB) [Novilysobacter ciconiae strain H21R20]
ATGAATGCCGTAGCTACCGCCAACCTTGTCGGCATCACCGGCATCGCCCGCCGCCTTGTACAGGACGGTGCTCTGTCCGA
GTCCGCCGCCCGCGAAGCCATGGCTGCTGCCACTGAGCAGCGCAAGCCGCTTGCCAGCTACATCATCGAGAAGCGACTGG
TCGCGCCCGCGCATCTGGCCGCCGCCAACTCAGTCGAGTTCGGCGTGCCGATCTTCGATGCCGCGGCGATGGACCCCGAG
CAGTCCGCGATCAAGCTGGTCAAGGAAGAGCTGCTGCGCAAGCACACGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTCGGCATCGCCGAGCCGACCAACACCCACGCGCTGGCGGAGATCAAGTTTCAGACCAACTTCACGGTCGAGGCGA
TCCTGGTCGATGAGGAGAGCATCAAGCGCCATCTGGATCGCTGGCTGGAAAGCGCCGACGCACTCGGCGACGTCATGGGC
GAGGATGACGAGGGCCTGGAGAACCTGGATATCGGTGGCGGGGAGGACGACCTGTCCGCTGATTCCGGGGTCGACGCCAA
GACCGACGACACTCCGGTGGTCAAGTTCATCAACAAGGTGCTGGTGGACGCTATCCGCCGCGGCGCCTCCGACATCCACT
TTGAGCCATACGAGACCGAATACCGCGTCCGGTTGCGCATCGACGGCCTGCTCAAGCAGTCGGCCAAGGTGCCGATCAAG
CTGCAGCCGCGCATCTCCGCGCGCCTGAAGGTCATGGCGCAGCTGGACATCGCCGAGCGCAGGGTGCCGCAAGACGGGCG
CATCAAGCTCAACCTGAGCAAAACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAACTCGGCTACGAGGACGACCAGCGCGACATTTTT
CTCGCCGCGGTCAAGCGTCCCTACGGCATGGTGCTGGTGACCGGTCCGACCGGCTCCGGCAAGACGGTGTCGCTCTACAC
CGCGCTGAACATCCTCAACGACGAGACCCGCAACATCTCCACGGTCGAGGACCCGGTCGAGATCCGCGTGCCGGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGTGAGATCCGCGACCTGGAGACCGCCGAGATCGGCATCAAGGCCGCACAGACAGGTCATATGGTGCTCTC
GACCCTGCATACCAACGACGCGCCACAGACCATCGCCCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCACCTCAT
CGGTGTCGCTGGTGATCGCCCAGCGTCTGGCGCGCCGCCTGCACAGCTGCAAACAGAAGATCGAACTGCCAGAGCATGCC
TTGCTGGCCGAGGGATTCACCGCGGAGGAAATCGCGGCGGAGGACTTCACGATCTACGAGGCGGTCGGGTGCGCCGACTG
CACCGAAGGCTACAAGGGCCGGACCGGCATCTACCAGGTCATGCCGATGACCGACGAGATCCAGGGCATCGTGCTGGCCG
GCGGGAACGCGATGCAGATCGCCGAGGCCGCGCAGAAGTCCGGCGTGCGCGACCTGCGCCAGTCGGCGCTGATGAAGGTG
CGCAACGGTGTCACCAGCCTGGCGGAGATCAACCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6UEZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.152

100

0.543
  pilB Acinetobacter baylyi ADP1

53.792

98.438

0.53
  pilB Legionella pneumophila strain ERS1305867

50.352

98.611

0.497
  pilB Vibrio cholerae strain A1552

52.529

89.236

0.469
  pilF Neisseria gonorrhoeae MS11

47.266

98.438

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

51.626

85.417

0.441
  pilB Vibrio campbellii strain DS40M4

50.607

85.764

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.081

100

0.384
  pilF Thermus thermophilus HB27

36.271

100

0.372