Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IOD66_RS03560 Genome accession   NZ_CP063424
Coordinates   746061..747455 (+) Length   464 a.a.
NCBI ID   WP_005601209.1    Uniprot ID   B0BPG4
Organism   Actinobacillus pleuropneumoniae strain APP5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 741061..752455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IOD66_RS03530 (IOD66_03555) - 741177..741953 (-) 777 WP_005597402.1 TSUP family transporter -
  IOD66_RS03535 (IOD66_03560) proB 741966..743057 (-) 1092 WP_005597401.1 glutamate 5-kinase -
  IOD66_RS03540 (IOD66_03565) - 743144..743632 (+) 489 WP_247797623.1 dihydrofolate reductase -
  IOD66_RS03545 (IOD66_03570) - 743759..743992 (+) 234 WP_005597399.1 YgjV family protein -
  IOD66_RS03550 (IOD66_03575) radA 744036..745415 (-) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  IOD66_RS03555 (IOD66_03580) pilA 745597..746043 (+) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  IOD66_RS03560 (IOD66_03585) pilB 746061..747455 (+) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  IOD66_RS03565 (IOD66_03590) pilC 747439..748641 (+) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  IOD66_RS03570 (IOD66_03595) - 748668..749306 (+) 639 WP_009874617.1 prepilin peptidase -
  IOD66_RS03575 (IOD66_03600) coaE 749328..749966 (+) 639 WP_009874616.1 dephospho-CoA kinase -
  IOD66_RS03580 (IOD66_03605) yacG 749948..750136 (+) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  IOD66_RS03585 (IOD66_03610) holA 750213..751238 (-) 1026 WP_009874615.1 DNA polymerase III subunit delta -
  IOD66_RS03590 (IOD66_03615) lptE 751347..751841 (-) 495 WP_005597385.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53022.90 Da        Isoelectric Point: 6.5042

>NTDB_id=495562 IOD66_RS03560 WP_005601209.1 746061..747455(+) (pilB) [Actinobacillus pleuropneumoniae strain APP5]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHLEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKTGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=495562 IOD66_RS03560 WP_005601209.1 746061..747455(+) (pilB) [Actinobacillus pleuropneumoniae strain APP5]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTGGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCGGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACTTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATAGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGACGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.192

98.922

0.675
  pilB Haemophilus influenzae Rd KW20

58.72

97.629

0.573
  pilB Haemophilus influenzae 86-028NP

58.278

97.629

0.569
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.412
  pilB Acinetobacter baumannii D1279779

38.855

100

0.409
  pilB Vibrio cholerae strain A1552

39.004

100

0.405
  pilB Acinetobacter baylyi ADP1

38.32

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369