Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IOD66_RS03555 Genome accession   NZ_CP063424
Coordinates   745597..746043 (+) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain APP5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 740597..751043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IOD66_RS03530 (IOD66_03555) - 741177..741953 (-) 777 WP_005597402.1 TSUP family transporter -
  IOD66_RS03535 (IOD66_03560) proB 741966..743057 (-) 1092 WP_005597401.1 glutamate 5-kinase -
  IOD66_RS03540 (IOD66_03565) - 743144..743632 (+) 489 WP_247797623.1 dihydrofolate reductase -
  IOD66_RS03545 (IOD66_03570) - 743759..743992 (+) 234 WP_005597399.1 YgjV family protein -
  IOD66_RS03550 (IOD66_03575) radA 744036..745415 (-) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  IOD66_RS03555 (IOD66_03580) pilA 745597..746043 (+) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  IOD66_RS03560 (IOD66_03585) pilB 746061..747455 (+) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  IOD66_RS03565 (IOD66_03590) pilC 747439..748641 (+) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  IOD66_RS03570 (IOD66_03595) - 748668..749306 (+) 639 WP_009874617.1 prepilin peptidase -
  IOD66_RS03575 (IOD66_03600) coaE 749328..749966 (+) 639 WP_009874616.1 dephospho-CoA kinase -
  IOD66_RS03580 (IOD66_03605) yacG 749948..750136 (+) 189 WP_005597388.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=495561 IOD66_RS03555 WP_005597397.1 745597..746043(+) (pilA) [Actinobacillus pleuropneumoniae strain APP5]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=495561 IOD66_RS03555 WP_005597397.1 745597..746043(+) (pilA) [Actinobacillus pleuropneumoniae strain APP5]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527