Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IOD66_RS03565 Genome accession   NZ_CP063424
Coordinates   747439..748641 (+) Length   400 a.a.
NCBI ID   WP_005601207.1    Uniprot ID   Q7BI81
Organism   Actinobacillus pleuropneumoniae strain APP5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742439..753641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IOD66_RS03540 (IOD66_03565) - 743144..743632 (+) 489 WP_247797623.1 dihydrofolate reductase -
  IOD66_RS03545 (IOD66_03570) - 743759..743992 (+) 234 WP_005597399.1 YgjV family protein -
  IOD66_RS03550 (IOD66_03575) radA 744036..745415 (-) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  IOD66_RS03555 (IOD66_03580) pilA 745597..746043 (+) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  IOD66_RS03560 (IOD66_03585) pilB 746061..747455 (+) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  IOD66_RS03565 (IOD66_03590) pilC 747439..748641 (+) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  IOD66_RS03570 (IOD66_03595) - 748668..749306 (+) 639 WP_009874617.1 prepilin peptidase -
  IOD66_RS03575 (IOD66_03600) coaE 749328..749966 (+) 639 WP_009874616.1 dephospho-CoA kinase -
  IOD66_RS03580 (IOD66_03605) yacG 749948..750136 (+) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  IOD66_RS03585 (IOD66_03610) holA 750213..751238 (-) 1026 WP_009874615.1 DNA polymerase III subunit delta -
  IOD66_RS03590 (IOD66_03615) lptE 751347..751841 (-) 495 WP_005597385.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 45588.09 Da        Isoelectric Point: 10.3239

>NTDB_id=495563 IOD66_RS03565 WP_005601207.1 747439..748641(+) (pilC) [Actinobacillus pleuropneumoniae strain APP5]
MLKVYEFHWKAKNRFQQLQKGKALACHRTELERRLLAKGYSQIRIQRNFRFVTQASSESITQVIKQLALLLNAAVPLKQA
LNMLLENTYHIRIYMWLNDLNRLIESGYTMSSSLTKLNLYLAKSEIQLVRIGEQSGRLGEILQNIAVNRHQTEKLHKKIK
KIMFYPVVTLIISVALSLAMLLFIVPQFIELYSGREKPLPLITQMLFTLSHFLQHSLLQVTIVSLLAVGFFRFFAKKYRF
LTAIKNGMLSALPIFNKIVENVRIVFFSQNLGLMLKAHIRLETALNTFLAAQSQDLRLQKEIENILAQLKQGYRFSESIN
ASVFGTQVVQMLAIGEQSGNVAQMCSYIGELYRQQLDDQIELLSQLLEPILMLIIGLIIGTVLIGLYLPIFDLGGLVGGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=495563 IOD66_RS03565 WP_005601207.1 747439..748641(+) (pilC) [Actinobacillus pleuropneumoniae strain APP5]
ATGTTGAAAGTGTATGAATTTCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGTTAGCTTGTCA
TCGAACGGAATTGGAACGGCGTTTATTAGCAAAAGGCTATTCACAGATCCGTATCCAACGTAATTTTCGTTTTGTCACAC
AGGCTTCTTCAGAATCGATTACTCAAGTAATTAAACAGTTGGCGCTTTTACTGAATGCCGCAGTCCCTTTAAAACAAGCG
CTGAATATGTTGTTAGAAAATACTTATCATATTCGGATCTATATGTGGCTGAATGATTTAAACCGTTTGATAGAATCGGG
TTATACAATGTCGTCAAGTTTAACCAAATTGAATTTATATTTGGCGAAATCGGAAATACAGCTGGTACGGATTGGCGAAC
AAAGCGGGCGATTAGGCGAAATTTTGCAAAATATTGCGGTAAATCGCCATCAAACCGAAAAATTACATAAGAAAATAAAA
AAGATAATGTTTTATCCTGTCGTCACTTTGATTATTTCCGTTGCGCTTTCTCTCGCCATGCTCCTTTTTATCGTGCCGCA
ATTTATCGAGCTGTATTCCGGTCGTGAAAAGCCGCTTCCTTTAATTACACAAATGCTATTTACATTATCGCACTTCTTAC
AACATTCTTTATTACAGGTAACGATAGTCAGTTTATTAGCGGTCGGTTTTTTCCGTTTTTTTGCAAAAAAATATCGATTT
TTAACCGCTATAAAAAACGGGATGTTAAGTGCTTTGCCTATTTTTAATAAGATAGTCGAAAACGTTCGTATAGTATTTTT
TAGTCAAAATCTCGGCTTGATGCTAAAAGCTCATATTCGGCTGGAAACGGCGTTAAATACCTTTCTTGCTGCACAATCGC
AAGATTTGAGGTTACAAAAAGAGATAGAAAATATTTTAGCGCAATTAAAACAAGGTTATCGCTTTTCCGAAAGTATTAAT
GCGAGTGTGTTCGGTACTCAAGTAGTACAAATGCTTGCCATTGGCGAACAGAGTGGGAATGTAGCGCAGATGTGTAGCTA
TATCGGGGAGTTATATCGACAACAATTGGATGATCAGATTGAACTGTTATCGCAGCTGCTTGAGCCGATCTTAATGCTGA
TTATCGGATTAATTATCGGTACGGTTTTGATCGGATTATATTTACCGATTTTTGATTTAGGCGGACTAGTCGGAGGCGGT
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7BI81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

50.126

99.25

0.498
  pilC Haemophilus influenzae Rd KW20

39.5

100

0.395
  pilC Haemophilus influenzae 86-028NP

38.5

100

0.385