Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IOD66_RS03550 Genome accession   NZ_CP063424
Coordinates   744036..745415 (-) Length   459 a.a.
NCBI ID   WP_005601211.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain APP5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 739036..750415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IOD66_RS03525 (IOD66_03550) nth 740544..741176 (-) 633 WP_005597404.1 endonuclease III -
  IOD66_RS03530 (IOD66_03555) - 741177..741953 (-) 777 WP_005597402.1 TSUP family transporter -
  IOD66_RS03535 (IOD66_03560) proB 741966..743057 (-) 1092 WP_005597401.1 glutamate 5-kinase -
  IOD66_RS03540 (IOD66_03565) - 743144..743632 (+) 489 WP_247797623.1 dihydrofolate reductase -
  IOD66_RS03545 (IOD66_03570) - 743759..743992 (+) 234 WP_005597399.1 YgjV family protein -
  IOD66_RS03550 (IOD66_03575) radA 744036..745415 (-) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  IOD66_RS03555 (IOD66_03580) pilA 745597..746043 (+) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  IOD66_RS03560 (IOD66_03585) pilB 746061..747455 (+) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  IOD66_RS03565 (IOD66_03590) pilC 747439..748641 (+) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  IOD66_RS03570 (IOD66_03595) - 748668..749306 (+) 639 WP_009874617.1 prepilin peptidase -
  IOD66_RS03575 (IOD66_03600) coaE 749328..749966 (+) 639 WP_009874616.1 dephospho-CoA kinase -
  IOD66_RS03580 (IOD66_03605) yacG 749948..750136 (+) 189 WP_005597388.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49678.24 Da        Isoelectric Point: 7.9094

>NTDB_id=495560 IOD66_RS03550 WP_005601211.1 744036..745415(-) (radA) [Actinobacillus pleuropneumoniae strain APP5]
MAKAPKTAYVCNDCGAEYSRWMGQCKECKSWNTVSEIRLISSKEATKSDRFSGYAGETSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLSKDLPTLYVTGEESLQQVAMRANRLGLPTDNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLADIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHCIDASI
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSDEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRALPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIIPHGNAPKKPIKGMEVFTVKKLSDALDILGNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=495560 IOD66_RS03550 WP_005601211.1 744036..745415(-) (radA) [Actinobacillus pleuropneumoniae strain APP5]
ATGGCTAAAGCACCAAAAACAGCTTATGTATGTAATGATTGCGGCGCGGAATATTCTCGTTGGATGGGGCAATGCAAAGA
ATGTAAGTCTTGGAATACCGTGAGTGAAATTAGACTTATCTCGAGTAAAGAAGCAACAAAAAGCGACCGCTTCAGCGGAT
ATGCCGGCGAAACCTCCGGTAAAGTTCAAACTTTATCCGAAATCAGCTTACAAGAAGTGCCTCGTTTTAGCAGCGGCTTT
AATGAATTGGATCGCGTACTGGGCGGCGGTGTCGTGCCGGGTTCGGCAATTTTAATCGGCGGCCATCCCGGTGCCGGTAA
ATCCACTCTGCTTTTACAAGTAATGTGCGGTTTGTCTAAAGATTTGCCGACCCTTTACGTGACTGGTGAAGAATCCCTGC
AACAAGTGGCGATGCGAGCGAATCGTTTAGGCTTACCGACCGACAATCTCAAGATGCTTTCAGAAACTTCGGTCGAGCAT
ATTTGTAATATCGCCGACCAAGAAAAGCCGAAAATTATGGTAATCGACTCAATTCAGGTCATGCACCTTGCCGATATTCA
ATCTTCTCCCGGTAGCGTTTCGCAAGTGCGGGAATGCGCTTCGTTTCTGACTCGCTATGCAAAAACCCGCCAAGTAGCGA
TTATTATGGTCGGACACGTCACCAAAGACGGGACACTTGCCGGCCCGAAAGTGTTGGAACACTGTATTGACGCCTCAATT
TTACTGGAAGGCGAATCGGACTCTCGATTCCGTACCTTACGCAGCCAGAAAAACCGTTTCGGTGCGGTCAATGAATTGGG
CGTATTTGCCATGACCGAACAAGGCTTACGAGAAGTAAAAAACCCTTCCGCGATTTTCCTCAGCCGTAGCGATGAACAAA
CGCCGGGCAGTTCCGTGATGGTGCTTTGGGAAGGAACCCGTCCGTTATTAGTCGAAATTCAAGCGTTGGTTGATCATTCG
ATGTTGGCTAATCCTCGCCGTGTCGCCGTAGGTTTAGATCACAACCGTTTATCGCTCTTACTTGCGGTGTTACATCGCCA
CGGCGGTTTGCAAATGTCGGATCAAGATGTGTTCGTCAATGTGGTCGGCGGGGTAAAAGTGACCGAGACCAGTGCCGATC
TCGCCCTAATTTTGGCGTTAATTTCCAGTTTTAGAAATCGTGCGTTACCGCAAGATTTGGTCGTATTCGGCGAGGTGGGA
TTAGCCGGAGAAATTCGTCCGGTACCGAGCGGTCAAGAACGTATCAGCGAAGCGGCGAAGCACGGTTTTAAACGTGCGAT
TATTCCTCACGGCAACGCACCTAAAAAACCGATTAAAGGTATGGAAGTCTTTACCGTTAAGAAACTAAGCGACGCACTAG
ATATTCTCGGCAATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.359

99.564

0.481
  radA Streptococcus mitis NCTC 12261

44.493

98.911

0.44
  radA Streptococcus mitis SK321

44.273

98.911

0.438
  radA Streptococcus pneumoniae Rx1

45.581

93.682

0.427
  radA Streptococcus pneumoniae D39

45.581

93.682

0.427
  radA Streptococcus pneumoniae R6

45.581

93.682

0.427
  radA Streptococcus pneumoniae TIGR4

45.581

93.682

0.427