Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   SPYALAB49_RS08665 Genome accession   NC_017596
Coordinates   1740953..1742224 (-) Length   423 a.a.
NCBI ID   WP_002992182.1    Uniprot ID   A0A660A3W1
Organism   Streptococcus pyogenes Alab49     
Function   require for competence (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1731231..1760305 1740953..1742224 within 0


Gene organization within MGE regions


Location: 1731231..1760305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYALAB49_RS08615 (SPYALAB49_001772) nrdG 1731231..1731845 (-) 615 WP_002982219.1 anaerobic ribonucleoside-triphosphate reductase activating protein -
  SPYALAB49_RS08620 (SPYALAB49_001773) - 1731860..1732354 (-) 495 WP_011055085.1 GNAT family N-acetyltransferase -
  SPYALAB49_RS08625 (SPYALAB49_001774) - 1732363..1733298 (-) 936 WP_002992828.1 Gfo/Idh/MocA family protein -
  SPYALAB49_RS09845 (SPYALAB49_001775) - 1733327..1733473 (-) 147 WP_002982210.1 hypothetical protein -
  SPYALAB49_RS08630 (SPYALAB49_001776) nrdD 1733655..1735853 (-) 2199 WP_002992831.1 anaerobic ribonucleoside-triphosphate reductase -
  SPYALAB49_RS08635 (SPYALAB49_001777) - 1735950..1737509 (-) 1560 WP_002992833.1 membrane protein -
  SPYALAB49_RS08640 (SPYALAB49_001778) - 1737922..1738227 (-) 306 WP_002982199.1 DUF1292 domain-containing protein -
  SPYALAB49_RS08645 (SPYALAB49_001779) ruvX 1738239..1738658 (-) 420 WP_002982196.1 Holliday junction resolvase RuvX -
  SPYALAB49_RS08650 (SPYALAB49_001780) - 1738655..1738924 (-) 270 WP_002982194.1 IreB family regulatory phosphoprotein -
  SPYALAB49_RS08655 (SPYALAB49_001781) spx 1739039..1739437 (-) 399 WP_002982188.1 transcriptional regulator Spx -
  SPYALAB49_RS08660 (SPYALAB49_001782) recA 1739728..1740864 (-) 1137 WP_002992179.1 recombinase RecA Machinery gene
  SPYALAB49_RS08665 (SPYALAB49_001783) cinA 1740953..1742224 (-) 1272 WP_002992182.1 competence/damage-inducible protein A Machinery gene
  SPYALAB49_RS08670 (SPYALAB49_001784) - 1742293..1742853 (-) 561 WP_002992183.1 DNA-3-methyladenine glycosylase I -
  SPYALAB49_RS08675 (SPYALAB49_001785) ruvA 1742863..1743459 (-) 597 WP_002992186.1 Holliday junction branch migration protein RuvA -
  SPYALAB49_RS08680 (SPYALAB49_001786) - 1743461..1744681 (-) 1221 WP_002992187.1 MFS transporter -
  SPYALAB49_RS08685 (SPYALAB49_001787) mutL 1744692..1746674 (-) 1983 WP_002992189.1 DNA mismatch repair endonuclease MutL -
  SPYALAB49_RS08690 (SPYALAB49_001788) - 1746769..1747920 (-) 1152 WP_014635780.1 site-specific integrase -
  SPYALAB49_RS10070 (SPYALAB49_001789) - 1748169..1748312 (+) 144 WP_011185066.1 putative holin-like toxin -
  SPYALAB49_RS08695 (SPYALAB49_001790) - 1749183..1749950 (-) 768 WP_003058809.1 helix-turn-helix transcriptional regulator -
  SPYALAB49_RS08700 (SPYALAB49_001791) - 1750104..1750310 (+) 207 WP_002992503.1 helix-turn-helix transcriptional regulator -
  SPYALAB49_RS08705 (SPYALAB49_001792) - 1750344..1751111 (+) 768 WP_010922759.1 Bro-N domain-containing protein -
  SPYALAB49_RS08710 (SPYALAB49_001793) - 1751121..1751744 (+) 624 WP_010922760.1 phage repressor protein -
  SPYALAB49_RS08715 (SPYALAB49_001794) - 1751771..1752013 (+) 243 WP_014635782.1 hypothetical protein -
  SPYALAB49_RS08725 (SPYALAB49_001795) - 1752271..1753404 (-) 1134 WP_000564846.1 ISAs1-like element IS1548 family transposase -
  SPYALAB49_RS08730 (SPYALAB49_001796) - 1753595..1753936 (+) 342 WP_010922762.1 hypothetical protein -
  SPYALAB49_RS08735 (SPYALAB49_001797) - 1753923..1754144 (+) 222 WP_014635783.1 hypothetical protein -
  SPYALAB49_RS08740 (SPYALAB49_001798) - 1754147..1754338 (+) 192 WP_010922764.1 hypothetical protein -
  SPYALAB49_RS08745 (SPYALAB49_001799) - 1754350..1754679 (+) 330 WP_003058810.1 hypothetical protein -
  SPYALAB49_RS08750 (SPYALAB49_001800) - 1754682..1754954 (+) 273 WP_010922766.1 hypothetical protein -
  SPYALAB49_RS08755 (SPYALAB49_001801) - 1754955..1755812 (+) 858 WP_010922767.1 primase alpha helix C-terminal domain-containing protein -
  SPYALAB49_RS08760 (SPYALAB49_001802) - 1755781..1757469 (+) 1689 WP_002992487.1 phage/plasmid primase, P4 family -
  SPYALAB49_RS09535 (SPYALAB49_001803) - 1757756..1757929 (+) 174 WP_000694577.1 hypothetical protein -
  SPYALAB49_RS09850 (SPYALAB49_001804) - 1757935..1758108 (+) 174 WP_014635784.1 hypothetical protein -
  SPYALAB49_RS08775 (SPYALAB49_001805) - 1758110..1758619 (+) 510 WP_014407945.1 hypothetical protein -
  SPYALAB49_RS08780 (SPYALAB49_001806) - 1758693..1759181 (+) 489 WP_010922769.1 hypothetical protein -
  SPYALAB49_RS08785 (SPYALAB49_001807) - 1759585..1759947 (+) 363 WP_010922770.1 DUF1492 domain-containing protein -
  SPYALAB49_RS08790 (SPYALAB49_001808) - 1759922..1760305 (+) 384 WP_010922771.1 ArpU family phage packaging/lysis transcriptional regulator -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45933.64 Da        Isoelectric Point: 4.7648

>NTDB_id=49556 SPYALAB49_RS08665 WP_002992182.1 1740953..1742224(-) (cinA) [Streptococcus pyogenes Alab49]
MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGDNEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQT
LAKYLRRDLVYDEQACQKLDDFFAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPI
VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTLRLSTKTENQALADKKLGQLEAQ
LLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKYDKSITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKM
LGLPLEELKSHGVVSAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLISGRSRLDV
CYIATLHAFNMVRKTLLKLENLL

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=49556 SPYALAB49_RS08665 WP_002992182.1 1740953..1742224(-) (cinA) [Streptococcus pyogenes Alab49]
ATGAAAGCTGAACTGATTGCAGTAGGTACCGAAATTTTGACTGGTCAAATTGTGAATACCAATGCTCAATTTCTGTCGGA
AAAAATGGCAGAGCTAGGTATTGATGTCTATTTTCAAACGGCTGTTGGGGACAACGAGGAGCGTTTACTTTCAGTGATTA
CAACTGCTAGTCAGCGGAGTGATTTGGTCATTTTATGCGGTGGCCTTGGTCCAACGAAAGATGATTTAACCAAACAAACT
TTAGCAAAGTACCTTAGGAGAGACTTGGTTTATGATGAGCAAGCTTGTCAGAAACTAGATGACTTTTTTGCTAAGCGCAA
GCCTTCATCACGGACACCAAATAATGAGCGACAGGCACAAGTGATTGAAGGATCAATCCCTTTGCCAAATAAAACTGGTC
TTGCGGTTGGTGGGTTCATTACAGTCGATGGTATTAGTTATGTTGTCTTACCGGGTCCTCCAAGTGAATTGAAGCCGATA
GTAAATGAAGAATTGGTACCACTTCTGTCAAAACAATACAGTACATTGTATTCAAAGGTACTACGCTTTTTTGGTATTGG
GGAAAGTCAGTTGGTAACAGTCTTGTCAGATTTTATTGAGAATCAAACTGATCCAACCATTGCTCCGTATGCTAAGACTG
GCGAAGTGACTCTTCGCTTATCAACAAAAACTGAAAACCAAGCTCTGGCAGATAAAAAGTTAGGTCAGCTAGAAGCGCAG
CTACTATCCCGAAAAACTCTTGAAGGTCAACCCTTAGCTGATGTCTTTTATGGCTATGGGGAGGATAATTCCTTAGCGCG
TGAGACATTTGAGCTCTTAGTAAAATATGATAAGTCAATTACAGCAGCAGAAAGTCTAACCGCGGGATTATTTCAGTCAA
CTTTGGCGAGTTTTCCAGGAGCTTCTCAAGTATTCAATGGAGGCTTTGTGACTTATAGCATGGAAGAAAAAGCGAAAATG
CTAGGCCTTCCTTTAGAGGAGTTGAAATCGCATGGCGTTGTTAGTGCTTATACGGCCGAGGGGATGGCGGAGCAAGCAAG
GTTATTGACTGGTGCTGATATTGGGGTAAGTTTAACAGGTGTTGCCGGACCAGATATGTTGGAGGAACAGCCTGCAGGTA
CAGTTTTCATTGGTCTTGCCACTCAAAATAAGGTAGAATCAATAAAGGTTTTGATTAGCGGGCGAAGTCGTTTGGATGTG
TGCTATATCGCTACTTTACATGCCTTTAATATGGTCCGTAAAACTTTATTAAAACTTGAGAATTTGCTATAA

Domains

Predicted by InterproScan.

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)

Damage-inducible protein CinA KH domain (CinA_KH)

(178-243)

Competence-damaged protein (CinA)

(263-416)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A660A3W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mutans UA159

69.267

100

0.693
  cinA Streptococcus mitis SK321

69.065

98.582

0.681
  cinA Streptococcus mitis NCTC 12261

68.585

98.582

0.676
  cinA Streptococcus pneumoniae TIGR4

67.626

98.582

0.667
  cinA Streptococcus pneumoniae Rx1

67.626

98.582

0.667
  cinA Streptococcus pneumoniae R6

67.626

98.582

0.667
  cinA Streptococcus pneumoniae D39

67.386

98.582

0.664
  cinA Streptococcus suis isolate S10

52.644

98.345

0.518
  cinA Bacillus subtilis subsp. subtilis str. 168

46.172

98.818

0.456

Multiple sequence alignment