Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IM687_RS05225 Genome accession   NZ_CP063358
Coordinates   1095425..1097128 (+) Length   567 a.a.
NCBI ID   WP_196112077.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain ZDHY95     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1090425..1102128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM687_RS05195 (IM687_05195) - 1090813..1091856 (-) 1044 WP_185745002.1 acyltransferase family protein -
  IM687_RS05200 (IM687_05200) - 1091880..1092764 (-) 885 WP_125883209.1 ABC transporter ATP-binding protein -
  IM687_RS05205 (IM687_05205) - 1092761..1093594 (-) 834 WP_196112074.1 hypothetical protein -
  IM687_RS05210 (IM687_05210) - 1093591..1094391 (-) 801 WP_196112075.1 hypothetical protein -
  IM687_RS05215 (IM687_05215) - 1094452..1094892 (-) 441 WP_196112076.1 hypothetical protein -
  IM687_RS21855 (IM687_05220) pilA 1094912..1095097 (-) 186 WP_272873510.1 type IV pilin protein Machinery gene
  IM687_RS05225 (IM687_05225) pilB 1095425..1097128 (+) 1704 WP_196112077.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IM687_RS05230 (IM687_05230) - 1097131..1098352 (+) 1222 Protein_1032 type II secretion system F family protein -
  IM687_RS05235 (IM687_05235) pilD 1098358..1099221 (+) 864 WP_196112078.1 prepilin peptidase Machinery gene
  IM687_RS05240 (IM687_05240) coaE 1099353..1099961 (+) 609 WP_196112079.1 dephospho-CoA kinase -
  IM687_RS05245 (IM687_05245) yacG 1099958..1100155 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  IM687_RS05250 (IM687_05250) - 1100168..1100848 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  IM687_RS05255 (IM687_05255) - 1100905..1101945 (-) 1041 WP_196112080.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62224.18 Da        Isoelectric Point: 5.3187

>NTDB_id=494639 IM687_RS05225 WP_196112077.1 1095425..1097128(+) (pilB) [Stutzerimonas stutzeri strain ZDHY95]
MNENLPLSGLARQLVLAGQLDEKVALQAQQQALRNQVPLVTWLVQNKLVKGRALVSVAGEQFGIPFFDLSVLDRDVQPRD
LVSEKLVRQHRVLPLQRRGNRLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDRLGEAIEQLFESAANSLDGMADSELD
DLVVGDVPDGADNATDHNDADDAPVVRFVNKMLLDAVRGGSSDLHFEPYEKSYRVRFRTDGVLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMRVSNAKSIDFRVNTLPTLWGEKIVMRILDSASAKMGIDALGYEDDQKDLYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKAEVEVPREVLLKEGFP
EASIGSFKVYAPVGCDSCKSGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARRDGFNDLRTSGLVKVMSGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=494639 IM687_RS05225 WP_196112077.1 1095425..1097128(+) (pilB) [Stutzerimonas stutzeri strain ZDHY95]
ATGAACGAAAATCTGCCCCTGAGTGGTTTGGCTCGCCAGTTGGTGCTGGCTGGACAGCTTGACGAAAAGGTCGCTCTCCA
AGCTCAGCAGCAAGCCTTGCGCAACCAGGTGCCGCTAGTGACTTGGCTGGTGCAGAACAAGTTGGTAAAAGGGCGGGCTT
TAGTAAGTGTCGCTGGCGAGCAGTTCGGTATCCCATTCTTCGATCTCTCCGTGCTTGATAGGGATGTTCAGCCTCGAGAC
TTGGTAAGCGAAAAGCTGGTTCGTCAGCACAGGGTGCTTCCACTGCAGCGTCGCGGTAACAGGCTTTATGTGGCGGTTTC
GGACCCTACTAACCACCAGGCGATCAGCGATATCCAGTTCAACACAGGTCTGTCCGTCGAGGCGGTCCTGGTTGAGGACG
ACAGGTTGGGTGAGGCCATCGAGCAGCTCTTTGAGTCTGCCGCCAATTCCCTGGATGGGATGGCGGATTCGGAGCTTGAT
GACCTTGTCGTCGGCGACGTCCCCGATGGCGCGGACAATGCGACCGATCACAATGACGCCGACGATGCGCCGGTCGTTCG
CTTCGTCAATAAGATGTTGCTTGATGCTGTCAGGGGTGGTTCTTCAGACTTACATTTCGAGCCGTATGAAAAAAGCTATC
GTGTTCGTTTTCGTACCGACGGCGTGTTGCATGAGGTTGCCAGGCCGCCAGTGCAATTGGTCAGTCGCATCTCGGCACGT
TTGAAGGTTATGGCCAGTCTTGATATCGCTGAGCGGCGAAAGCCTCAAGATGGGCGGATTAAAATGAGGGTTTCCAATGC
CAAGTCGATCGATTTCCGCGTGAATACGCTGCCTACGCTTTGGGGCGAAAAGATCGTAATGCGGATATTGGACTCTGCCA
GCGCAAAGATGGGAATCGATGCTTTGGGCTACGAGGATGACCAGAAGGACCTCTATCTGGCTGCGCTGAAGCAGCCGCAG
GGTATGATATTGGTTACAGGTCCGACTGGTTCTGGAAAAACCGTTTCGCTATATACCGGTCTGAATATTCTGAATACGGT
CGATGTTAATATCTCTACGGCCGAAGATCCGGTCGAGATAAATCTGGAGGGGATCAACCAGGTCAACGTGAATCCTAGAC
AAGGCATGGACTTCGCCCAAGCTCTACGCGCATTTCTGCGCCAGGATCCGGATGTCATCATGGTGGGCGAAATCCGGGAT
TTGGAAACTGCCGAAATTGCTGTCAAGGCGGCGCAAACTGGCCATATGGTGATGTCCACGTTGCATACCAATAGCGCCGC
AGAGACGCTGACCCGACTCCGCAATATGGGCGTGCCGGCCTTCAATATCGCGACCTCTGTGAATCTGATTATCGCCCAGC
GGTTGGCGCGTAAGCTATGTCCATCCTGCAAGGCTGAGGTTGAGGTCCCGCGTGAGGTTTTGTTGAAGGAGGGGTTCCCA
GAGGCCAGCATCGGGTCGTTCAAGGTCTACGCTCCGGTCGGGTGCGACAGTTGTAAGTCGGGCTACAAAGGGCGTGTCGG
TATTTATGAAGTAGTTAAAATTACGCCAGCCCTGCAACGGCTTATCATGGAGGAAGGCAATTCCATCGACATCGCCTTAC
AGGCGCGCAGAGATGGATTTAACGACCTTCGCACTTCGGGACTGGTTAAGGTCATGAGTGGCATTACCAGCTTGGAAGAA
GTCAATCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.437

99.295

0.58
  pilB Acinetobacter baylyi ADP1

57.624

99.471

0.573
  pilB Legionella pneumophila strain ERS1305867

53.671

100

0.541
  pilB Vibrio cholerae strain A1552

55.03

89.418

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

54.331

89.594

0.487
  pilB Vibrio campbellii strain DS40M4

50.642

96.12

0.487
  pilF Neisseria gonorrhoeae MS11

52.838

90.123

0.476
  pilB/pilB1 Synechocystis sp. PCC 6803

38.126

100

0.416
  pilF Thermus thermophilus HB27

38.434

99.118

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.626

94.356

0.374