Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   INP89_RS08760 Genome accession   NZ_CP063127
Coordinates   1758981..1759430 (+) Length   149 a.a.
NCBI ID   WP_012054948.1    Uniprot ID   A0AAX3ITG0
Organism   Haemophilus influenzae strain M1C112_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1753981..1764430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP89_RS08740 (INP89_08735) rsmE 1754442..1755179 (-) 738 WP_012054951.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  INP89_RS08745 (INP89_08740) lnt 1755229..1756758 (-) 1530 WP_074039664.1 apolipoprotein N-acyltransferase -
  INP89_RS08750 (INP89_08745) corC 1756781..1757680 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  INP89_RS08755 (INP89_08750) ampD 1758303..1758866 (-) 564 WP_012054949.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP89_RS08760 (INP89_08755) pilA 1758981..1759430 (+) 450 WP_012054948.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  INP89_RS08765 (INP89_08760) pilB 1759427..1760821 (+) 1395 WP_012054947.1 GspE/PulE family protein Machinery gene
  INP89_RS08770 (INP89_08765) pilC 1760818..1762038 (+) 1221 WP_012054946.1 type II secretion system F family protein Machinery gene
  INP89_RS08775 (INP89_08770) pilD 1762035..1762727 (+) 693 WP_005666688.1 prepilin peptidase Machinery gene
  INP89_RS08780 (INP89_08775) rho 1762782..1764044 (-) 1263 WP_005666690.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15523.99 Da        Isoelectric Point: 9.6179

>NTDB_id=493247 INP89_RS08760 WP_012054948.1 1758981..1759430(+) (pilA) [Haemophilus influenzae strain M1C112_1]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSTCSGGSNGIA
ADITTAKGYVASVKTQSGGITVKGNGTLANMEYILQAKGNATAGVTWTTTCKGTDASLFPANFCRSVTK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=493247 INP89_RS08760 WP_012054948.1 1758981..1759430(+) (pilA) [Haemophilus influenzae strain M1C112_1]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCTTCTGCGC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGCAAACCTTCTACTTGCTCAGGAGGAAGCAATGGAATTGCA
GCTGATATTACAACAGCAAAAGGCTATGTAGCCTCAGTGAAAACTCAATCAGGTGGTATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTAAAGGTAATGCTACAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCAGAAGTGTCACAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

91.946

100

0.919
  pilA Haemophilus influenzae 86-028NP

87.919

100

0.879
  pilA Glaesserella parasuis strain SC1401

56.757

99.329

0.564
  pilA2 Legionella pneumophila strain ERS1305867

39.735

100

0.403
  pilA2 Legionella pneumophila str. Paris

40.559

95.973

0.389
  comP Acinetobacter baylyi ADP1

38.621

97.315

0.376
  pilA Vibrio campbellii strain DS40M4

39.007

94.631

0.369