Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INP93_RS00260 Genome accession   NZ_CP063121
Coordinates   52570..53949 (+) Length   459 a.a.
NCBI ID   WP_197544813.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C130_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 47570..58949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP93_RS00230 (INP93_00230) - 47863..48714 (-) 852 WP_049364941.1 thymidylate synthase -
  INP93_RS00235 (INP93_00235) lgt 48728..49534 (-) 807 WP_070582537.1 prolipoprotein diacylglyceryl transferase -
  INP93_RS00240 (INP93_00240) - 49543..50337 (-) 795 WP_197544810.1 sulfite exporter TauE/SafE family protein -
  INP93_RS00245 (INP93_00245) rppH 50337..50930 (-) 594 WP_049379955.1 RNA pyrophosphohydrolase -
  INP93_RS00250 (INP93_00250) ampD 51414..51977 (-) 564 WP_197544811.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP93_RS00255 (INP93_00255) pilA 52120..52563 (+) 444 WP_197544812.1 prepilin peptidase-dependent pilin Machinery gene
  INP93_RS00260 (INP93_00260) pilB 52570..53949 (+) 1380 WP_197544813.1 GspE/PulE family protein Machinery gene
  INP93_RS00265 (INP93_00265) pilC 53958..55181 (+) 1224 WP_197544814.1 type II secretion system F family protein Machinery gene
  INP93_RS00270 (INP93_00270) pilD 55178..55855 (+) 678 WP_197544815.1 prepilin peptidase Machinery gene
  INP93_RS00275 (INP93_00275) coaE 55944..56564 (+) 621 WP_197544816.1 dephospho-CoA kinase -
  INP93_RS00280 (INP93_00280) yacG 56557..56763 (+) 207 WP_197544817.1 DNA gyrase inhibitor YacG -
  INP93_RS00285 (INP93_00285) - 56760..57041 (+) 282 WP_197544818.1 GNAT family N-acetyltransferase -
  INP93_RS00290 (INP93_00290) rhlB 57132..58394 (+) 1263 WP_197544819.1 ATP-dependent RNA helicase RhlB -
  INP93_RS00295 (INP93_00295) mreD 58436..58924 (-) 489 WP_193451403.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52026.32 Da        Isoelectric Point: 6.0530

>NTDB_id=493094 INP93_RS00260 WP_197544813.1 52570..53949(+) (pilB) [Haemophilus parainfluenzae strain M1C130_2]
MAYQAIAKNGEIYQISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFDTPQLK
QLLQSLAPKTNQIEEQTTFYHHSEDENSANLANDDEPVIQLLNGIFETALQKNASDIHLETQPSGLLIRLRIDGVLQPQP
IISKALANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQN
QQSQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
SDSCDCHQGYQGRIGVYQFLQCENNRYQTDFESLYQSALEKVKDRITDLAEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=493094 INP93_RS00260 WP_197544813.1 52570..53949(+) (pilB) [Haemophilus parainfluenzae strain M1C130_2]
ATGGCTTATCAAGCAATCGCAAAAAATGGCGAGATTTATCAGATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTGCTGTTGCGTTACTTTGCGCTCCCTCTTAAAGAAGACGAACACCATTTATGGCTTGCAGTGGACTCACTCAATA
ATCTTGCAGCTTGTGAAACCTTTGCTTTTTTGAGCGGAAAATTAGTTGAACCGATTTTATTTGACACGCCCCAACTCAAA
CAACTTTTACAATCCCTTGCACCTAAAACCAATCAAATCGAAGAGCAAACGACGTTTTACCATCATTCTGAAGATGAAAA
CTCAGCTAATTTAGCTAATGATGACGAACCCGTTATTCAATTATTAAATGGTATTTTTGAAACAGCCCTACAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGCCAAGTGGGCTCTTAATTCGTTTACGTATTGATGGAGTGCTCCAACCGCAGCCG
ATTATCAGTAAAGCGCTCGCCAATCGTGTAATTTCTCGCCTTAAACTCTTAGCCAAACTGGATATTAGTGAAACTCGATT
ACCACAAGATGGCCGCTTTCAATTCAAAACGACGTTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACACATT
GGGGAGAAAAAGCGGTATTACGATTACAACAAAATAAACCTGTTCAGTTAAGTTTTGCTGAACTTGGCATGACACAAAAC
CAACAAAGTCAATTTCAACATGCCCTCAGCCAACCTCAAGGTTTAATCCTTGTGACCGGCCCCACGGGAAGCGGAAAAAG
CATCTCGCTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACGGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGCATCATTCAAACTCAAGTAAATCCACAAATCGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGG
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGACGAAGAAAGTGCATTGATGGCATTAAGAGCGGCTCAAACAGG
TCATCTAGTACTTTCTACCTTACATACCAACGATGCGATCTCAGCCATTTCACGCTTACAACAATTAGGCATTCAATCAC
ATGAAATTGAAAACAGTCTCTTATTAGTTATCGCACAACGATTGGTACGCAAACGTTGCTTAAAGTGCGGCCAACATTTT
AGTGATTCTTGCGATTGCCATCAGGGATATCAAGGTCGTATTGGGGTATATCAATTTTTACAATGCGAAAACAACCGTTA
CCAAACTGATTTTGAATCACTTTATCAAAGTGCGTTAGAAAAAGTGAAAGATCGCATCACCGATCTCGCCGAAATTCAAC
GCGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-88)

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

78.319

98.475

0.771
  pilB Haemophilus influenzae Rd KW20

76.923

99.129

0.763
  pilB Glaesserella parasuis strain SC1401

59.513

98.475

0.586
  pilB Vibrio campbellii strain DS40M4

41.322

100

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

41.25

100

0.431
  pilB Vibrio cholerae strain A1552

41.042

100

0.429
  pilB Acinetobacter baumannii D1279779

39.22

100

0.416
  pilB Acinetobacter baylyi ADP1

38.229

100

0.414
  pilB Legionella pneumophila strain ERS1305867

38.241

100

0.407
  pilF Neisseria gonorrhoeae MS11

36.611

100

0.381