Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   INP81_RS03985 Genome accession   NZ_CP063057
Coordinates   881426..882643 (-) Length   405 a.a.
NCBI ID   WP_003062193.1    Uniprot ID   A0A5S4SYV6
Organism   Comamonas thiooxydans strain ZDHYF418     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 876426..887643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP81_RS03955 (INP81_03955) - 877235..878170 (+) 936 WP_054073468.1 ATP-binding protein -
  INP81_RS03960 (INP81_03960) - 878183..878653 (+) 471 WP_054073469.1 NUDIX domain-containing protein -
  INP81_RS03965 (INP81_03965) - 878771..878980 (-) 210 WP_039049611.1 DNA gyrase inhibitor YacG -
  INP81_RS03970 (INP81_03970) zapD 879084..879839 (-) 756 WP_039049612.1 cell division protein ZapD -
  INP81_RS03975 (INP81_03975) coaE 879927..880553 (-) 627 WP_039049613.1 dephospho-CoA kinase -
  INP81_RS03980 (INP81_03980) - 880557..881426 (-) 870 WP_003062192.1 prepilin peptidase -
  INP81_RS03985 (INP81_03985) pilC 881426..882643 (-) 1218 WP_003062193.1 type II secretion system F family protein Machinery gene
  INP81_RS03990 (INP81_03990) pilB 882668..884410 (-) 1743 WP_039049614.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INP81_RS04000 (INP81_04000) - 884664..885593 (-) 930 WP_003062198.1 polyprenyl synthetase family protein -
  INP81_RS04005 (INP81_04005) rplU 885939..886250 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  INP81_RS04010 (INP81_04010) rpmA 886267..886524 (+) 258 WP_003058534.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44513.26 Da        Isoelectric Point: 9.6093

>NTDB_id=492621 INP81_RS03985 WP_003062193.1 881426..882643(-) (pilC) [Comamonas thiooxydans strain ZDHYF418]
MASAASKGIKEFLFEWEGKDRNGKIVRGETRAGGENQIQAMLRRQGVTPSKIKKRRTRGGKKIKPKDIALFTRQLATMMK
AGVPLLQSFDIVGRGNTNPNVTKLLNDIRSDVETGTSLSAAFRKFPLYFNSLYCNLVEAGEAAGILESLLDRLATYMEKT
EAIKSKIKSALMYPTSVMIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMGISDYFVQYWWLIFGVLGGGIYFFM
QAWKRNERVQQFMDRTILKLPIFGVLIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNYLYKNATDRIQSEVSTGT
SLTVAMANANIFPSMVLQMCAIGEESGAIDHMLGKAADFYESEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=492621 INP81_RS03985 WP_003062193.1 881426..882643(-) (pilC) [Comamonas thiooxydans strain ZDHYF418]
ATGGCAAGCGCAGCGTCCAAGGGAATCAAGGAATTTCTCTTCGAGTGGGAAGGCAAGGACCGCAACGGCAAGATCGTTCG
CGGCGAGACTCGTGCCGGCGGAGAAAACCAGATCCAGGCCATGCTGCGCCGCCAGGGCGTGACTCCGTCTAAGATCAAGA
AGCGCAGGACCCGCGGCGGCAAGAAGATCAAGCCCAAGGACATTGCGCTGTTCACGCGCCAGCTGGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCTTTCGACATCGTGGGCCGGGGCAACACCAACCCCAACGTCACCAAGCTGCTCAACGA
CATCCGCTCCGATGTGGAAACCGGCACTTCGCTGAGCGCCGCCTTTCGCAAGTTTCCGCTCTATTTCAACAGCCTCTACT
GCAATCTGGTGGAGGCCGGCGAGGCCGCAGGTATTCTGGAATCGTTGCTGGACCGTCTTGCCACCTATATGGAAAAGACG
GAAGCCATCAAGTCCAAGATCAAGTCGGCGCTGATGTACCCCACGTCGGTCATGATCGTGGCCTTCGTCGTGGTGACCGT
GATCATGATCTTCGTGATCCCGGCCTTCAAGGAGGTCTTCACCTCCTTTGGCGCCGACCTGCCCGCGCCCACGCTGCTGG
TGATGGGCATCAGCGATTACTTTGTCCAGTACTGGTGGCTGATCTTCGGCGTGCTGGGCGGCGGCATCTATTTCTTCATG
CAGGCCTGGAAGCGCAACGAGCGCGTACAGCAGTTCATGGACCGCACCATACTCAAGCTGCCCATCTTCGGCGTGCTGAT
CGAGAAGTCCTGCGTGGCCCGCTGGACACGTACGCTGTCCACTATGTTTGCCGCCGGCGTGCCGCTGGTCGAGGCGCTGG
ACTCCGTGGGAGGCGCCTCGGGCAACTACCTCTACAAAAACGCCACCGACAGGATTCAGTCGGAAGTCTCCACGGGCACC
AGCCTGACCGTGGCCATGGCCAATGCCAATATCTTCCCTTCCATGGTGCTGCAGATGTGTGCCATCGGCGAGGAATCGGG
CGCCATCGACCATATGCTGGGCAAGGCAGCCGATTTCTATGAAAGCGAAGTCGACGAAATGGTGGCGGGCCTCTCCAGCC
TGATGGAGCCCATCATCATCGTCTTCCTGGGCACCTTGATCGGCGGCATCGTGGTGTCCATGTATCTGCCTATCTTCAAG
CTGGGTCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4SYV6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.465

99.753

0.533
  pilG Neisseria gonorrhoeae MS11

51.889

98.025

0.509
  pilG Neisseria meningitidis 44/76-A

51.637

98.025

0.506
  pilC Legionella pneumophila strain ERS1305867

50.758

97.778

0.496
  pilC Acinetobacter baylyi ADP1

49.749

98.272

0.489
  pilC Acinetobacter baumannii D1279779

48.363

98.025

0.474
  pilC Vibrio cholerae strain A1552

40.302

98.025

0.395
  pilC Vibrio campbellii strain DS40M4

38.06

99.259

0.378
  pilC Thermus thermophilus HB27

36.634

99.753

0.365