Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ICN73_RS08260 Genome accession   NZ_CP062699
Coordinates   1813981..1815243 (+) Length   420 a.a.
NCBI ID   WP_017148006.1    Uniprot ID   A0A7L9GK88
Organism   Pseudomonas taiwanensis strain WRS8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1808981..1820243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICN73_RS08245 (ICN73_08245) pilM 1811630..1812511 (+) 882 WP_017148007.1 type IV pilus biogenesis protein PilM -
  ICN73_RS08250 (ICN73_08250) - 1812508..1813044 (+) 537 WP_012053951.1 PilN domain-containing protein -
  ICN73_RS08255 (ICN73_08255) - 1813056..1814057 (+) 1002 WP_192907707.1 pilus assembly protein PilP -
  ICN73_RS08260 (ICN73_08260) pilQ 1813981..1815243 (+) 1263 WP_017148006.1 type IV pilus secretin PilQ Machinery gene
  ICN73_RS08265 (ICN73_08265) aroK 1815248..1815766 (+) 519 WP_010955625.1 shikimate kinase AroK -
  ICN73_RS08270 (ICN73_08270) aroB 1815863..1816960 (+) 1098 WP_003249149.1 3-dehydroquinate synthase -
  ICN73_RS08275 (ICN73_08275) - 1816976..1818577 (+) 1602 WP_003249151.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45716.39 Da        Isoelectric Point: 4.9839

>NTDB_id=490561 ICN73_RS08260 WP_017148006.1 1813981..1815243(+) (pilQ) [Pseudomonas taiwanensis strain WRS8]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQVLDAQLQPLRRELLTIHHAKAADLAQLLLASLEGGGISTGSLSVDERT
NTLVVYLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNRV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=490561 ICN73_RS08260 WP_017148006.1 1813981..1815243(+) (pilQ) [Pseudomonas taiwanensis strain WRS8]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTTG
CCAGTGATGCGGTACAAGGCAGCGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTCGACCTGGTGTTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTCTTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGCCAAGTGCTGGACGCGCAGCTGCAGCCATTGCGTCGGGAGCTGCTGACCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCTGGCAAGTCTGGAGGGTGGCGGTATCTCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGTTGGTGGCGCAGTTGGACGTGCCGGTGCG
TCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAACGAGCCGGCAGCAGTATCGGCCTG
GGTCTGTTGCGCGGTGACGTGCTGCTGGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACGAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTCGAAGTAACACCACAGATCACTCCGGACAACAGGGTG
ATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCGTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(126-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L9GK88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.633

100

0.619
  comQ Acinetobacter baylyi ADP1

42.667

100

0.457
  pilQ Acinetobacter baumannii strain A118

40.781

100

0.448
  pilQ Acinetobacter baumannii D1279779

40.781

100

0.448
  pilQ Legionella pneumophila strain ERS1305867

43.029

99.048

0.426
  pilQ Legionella pneumophila strain Lp02

43.029

99.048

0.426
  pilQ Vibrio campbellii strain DS40M4

41.244

100

0.426
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.651

98.81

0.421
  pilQ Vibrio cholerae strain A1552

42.651

98.81

0.421