Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IF654_RS04380 Genome accession   NZ_CP062158
Coordinates   856783..857988 (-) Length   401 a.a.
NCBI ID   WP_194862741.1    Uniprot ID   -
Organism   Pseudomonas lundensis strain 2T.2.5.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 851783..862988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF654_RS04345 (IF654_06575) - 852824..852997 (-) 174 WP_047273641.1 DUF3094 family protein -
  IF654_RS04350 (IF654_06570) - 853053..853580 (-) 528 WP_094987426.1 MOSC domain-containing protein -
  IF654_RS04355 (IF654_06565) - 853577..854206 (-) 630 WP_047273644.1 DUF1780 domain-containing protein -
  IF654_RS04360 (IF654_06560) - 854338..855027 (+) 690 WP_047283079.1 energy-coupling factor ABC transporter permease -
  IF654_RS04365 (IF654_06555) yacG 855095..855298 (-) 204 WP_047279000.1 DNA gyrase inhibitor YacG -
  IF654_RS04370 (IF654_06550) coaE 855295..855918 (-) 624 WP_047281317.1 dephospho-CoA kinase -
  IF654_RS04375 (IF654_06545) pilD 855920..856780 (-) 861 WP_269468886.1 prepilin peptidase Machinery gene
  IF654_RS04380 (IF654_06540) pilC 856783..857988 (-) 1206 WP_194862741.1 type II secretion system F family protein Machinery gene
  IF654_RS04385 (IF654_06535) - 858212..858619 (+) 408 WP_047273654.1 pilin -
  IF654_RS04395 (IF654_06525) - 858888..860387 (+) 1500 WP_194862740.1 DNA-3-methyladenine glycosylase 2 family protein -
  IF654_RS04400 (IF654_06520) - 860390..860887 (+) 498 WP_047278992.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  IF654_RS04405 (IF654_06515) nadC 860909..861757 (-) 849 WP_047274271.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 43483.07 Da        Isoelectric Point: 10.3516

>NTDB_id=486558 IF654_RS04380 WP_194862741.1 856783..857988(-) (pilC) [Pseudomonas lundensis strain 2T.2.5.2]
MADTYLYRWEGKDAHGVTHTGELSASGHAQLRAQLRRKGITVRSIRRQRTSRRRAGRSVNASDITLFSRQLATLIQSGVP
LLQALEVIGKGFEQCAMRQLMIELTRDIAAGTSLTAALRKKPEHFDTLFCNLVEAGEQAGALEVMLQRIATYKEKSDALK
KKIKKAMTYPIAVLLVALVVSGILLIKVVPQFQGVFASFNAQLPVFTLWVIGMSEALQRHGLWLLLGTAGAAVIVRHRYK
ASATLRHRLDALLLKIPVTGQLLHLSAIARFARTLSTTLAAGVPLLDALDSVAGATGNSVLKEAVLRLKQHVATGTQIHV
AMSSSGAFPDMAIQMTAIGEESGTLDSMLNHVATYYESEVDTLVDNLTTLMEPAIMVVLGIVVGGLVIAMYLPIFKLGQV
I

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=486558 IF654_RS04380 WP_194862741.1 856783..857988(-) (pilC) [Pseudomonas lundensis strain 2T.2.5.2]
ATGGCCGACACGTATTTGTATCGCTGGGAAGGCAAGGATGCCCACGGCGTCACTCACACCGGGGAACTCAGCGCCAGCGG
GCACGCCCAGCTTCGCGCCCAATTGCGGCGCAAAGGGATCACGGTGCGCAGCATTCGGCGTCAACGCACCTCACGCCGTC
GCGCTGGCAGGTCTGTCAACGCGTCAGACATCACCCTGTTCAGTCGCCAACTGGCGACGCTGATCCAGTCGGGCGTGCCT
CTTTTGCAAGCGCTGGAGGTAATTGGCAAAGGGTTCGAGCAGTGCGCCATGCGCCAACTGATGATCGAACTGACGCGGGA
CATCGCCGCCGGAACCAGCCTCACCGCCGCCCTGCGTAAAAAACCGGAGCATTTCGACACGCTGTTCTGCAATCTGGTGG
AAGCCGGGGAACAAGCAGGCGCGCTGGAGGTGATGCTGCAACGTATCGCGACCTACAAGGAAAAATCCGACGCGCTGAAG
AAGAAGATCAAAAAAGCCATGACGTATCCCATCGCCGTGTTGCTGGTGGCATTAGTGGTCAGCGGCATCCTGTTGATAAA
AGTCGTGCCGCAATTTCAGGGGGTGTTTGCTTCGTTCAATGCGCAACTCCCCGTCTTTACCTTGTGGGTGATTGGCATGT
CAGAGGCATTGCAACGCCACGGCCTTTGGCTTTTACTGGGCACCGCTGGCGCTGCCGTGATCGTCCGTCATCGTTACAAG
GCCTCAGCCACCCTGCGCCATCGACTGGACGCCCTGTTGCTGAAAATCCCTGTCACCGGGCAGTTATTACACCTGTCTGC
GATTGCCCGATTTGCCCGCACCCTTTCCACCACCCTGGCCGCCGGCGTGCCTTTGCTCGACGCGCTGGACTCAGTCGCAG
GCGCGACGGGCAATAGTGTGCTGAAAGAGGCCGTGCTGCGGCTCAAGCAACACGTTGCCACGGGTACGCAGATTCATGTG
GCCATGAGCAGCAGTGGCGCATTCCCGGACATGGCGATTCAGATGACAGCCATTGGCGAAGAGTCCGGCACACTGGACAG
CATGCTCAATCACGTAGCCACCTATTATGAGAGCGAGGTCGACACCCTCGTCGATAACCTGACAACGCTGATGGAGCCCG
CAATCATGGTGGTACTGGGGATTGTGGTGGGCGGTCTGGTCATTGCCATGTATTTACCGATTTTCAAACTGGGCCAAGTG
ATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(271-393)

Type II secretion system (T2SS), protein F (T2SSF)

(67-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.566

98.753

0.559
  pilC Acinetobacter baumannii D1279779

51.637

99.002

0.511
  pilC Acinetobacter baylyi ADP1

51.005

99.252

0.506
  pilC Legionella pneumophila strain ERS1305867

47.739

99.252

0.474
  pilC Vibrio cholerae strain A1552

41.919

98.753

0.414
  pilG Neisseria gonorrhoeae MS11

42.298

95.511

0.404
  pilG Neisseria meningitidis 44/76-A

42.037

95.511

0.402
  pilC Vibrio campbellii strain DS40M4

38.693

99.252

0.384