Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ID616_RS28000 Genome accession   NZ_CP061723
Coordinates   6162037..6163299 (-) Length   420 a.a.
NCBI ID   WP_191087069.1    Uniprot ID   -
Organism   Pseudomonas putida strain ZXPA-20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6157037..6168299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ID616_RS27980 (ID616_27980) - 6158346..6158570 (-) 225 WP_132844974.1 hypothetical protein -
  ID616_RS27985 (ID616_27985) - 6158700..6160304 (-) 1605 WP_191087068.1 SPOR domain-containing protein -
  ID616_RS27990 (ID616_27990) aroB 6160320..6161417 (-) 1098 WP_070086873.1 3-dehydroquinate synthase -
  ID616_RS27995 (ID616_27995) aroK 6161514..6162032 (-) 519 WP_024717571.1 shikimate kinase AroK -
  ID616_RS28000 (ID616_28000) pilQ 6162037..6163299 (-) 1263 WP_191087069.1 type IV pilus secretin PilQ Machinery gene
  ID616_RS28005 (ID616_28005) - 6163223..6164239 (-) 1017 WP_095116155.1 pilus assembly protein PilP -
  ID616_RS28010 (ID616_28010) - 6164236..6164772 (-) 537 WP_191087070.1 PilN domain-containing protein -
  ID616_RS28015 (ID616_28015) pilM 6164769..6165650 (-) 882 WP_023048611.1 type IV pilus biogenesis protein PilM -
  ID616_RS28020 (ID616_28020) - 6165845..6168295 (+) 2451 WP_371918298.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45978.71 Da        Isoelectric Point: 4.9075

>NTDB_id=484406 ID616_RS28000 WP_191087069.1 6162037..6163299(-) (pilQ) [Pseudomonas putida strain ZXPA-20]
MKNVMKWVLAALLPIPLVWATPYQGEPLSLNFQDVEVRSVLQVLADYAGINLVASDDVQGNVTLRLQDVPWDQALDLVLR
SKGLAQRQEGNVLLVAPAAAFAPQPFDAHPVPDAQMQPLRRELIPIHHAKAAELVELLLTSLADDSTFTVRGSLGVDERT
NTLVAHQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYRESLRPGKELFVDLGVERAGSSVGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVAFREASLSLEVTPQITPDNKV
IMAVRVTKDEPDYVNAVNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDVLQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=484406 ID616_RS28000 WP_191087069.1 6162037..6163299(-) (pilQ) [Pseudomonas putida strain ZXPA-20]
ATGAAGAACGTGATGAAATGGGTGTTGGCGGCCCTGCTGCCGATCCCGCTGGTGTGGGCGACGCCGTATCAGGGTGAGCC
ACTTTCTTTGAATTTCCAGGATGTAGAGGTTCGCTCGGTGCTGCAGGTGCTGGCCGATTACGCGGGCATCAATCTGGTTG
CCAGCGATGACGTGCAGGGCAACGTGACCTTGCGTTTGCAGGACGTGCCCTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGGCACAGCGGCAGGAAGGCAATGTGTTGCTGGTGGCCCCTGCGGCAGCGTTTGCCCCGCAGCCGTTCGA
TGCCCATCCGGTGCCGGATGCGCAGATGCAGCCGTTACGCCGTGAACTGATACCGATCCATCATGCCAAGGCGGCGGAGC
TGGTTGAGTTGTTGCTGACCAGCCTGGCGGATGACAGCACCTTCACCGTTCGTGGCAGCCTGGGTGTCGATGAGCGCACC
AATACCCTGGTGGCGCATCAGCCGGCCGATCGCCTGGCCGAATTGCGGCAACTGGTTGCGCAGCTGGACGTGCCGGTGCG
CCAGGTGGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGACTACGAGAAAAGCCTGGGGGTGCGCTGGGGCGGGCCGC
TGTACAGGGAAAGCCTGCGCCCGGGCAAGGAACTGTTCGTCGACCTCGGCGTGGAGCGGGCGGGCAGCAGCGTCGGCCTG
GGCCTGCTGCGTGGCGACGTGTTGCTCGACCTGGAACTCAGCGCCATGGAAAAAAGCGGCAACGGCGAGATCATCTCGCA
GCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAGGGCACCGAGGTGCCCTACCAGGAAACCAGCCAGA
GTGGCGCCACCTCGGTCGCCTTCCGCGAAGCCTCGCTTTCGCTGGAGGTGACCCCGCAGATTACACCTGACAACAAGGTA
ATCATGGCTGTCAGGGTGACCAAGGACGAGCCTGATTACGTCAATGCCGTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(123-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.81

100

0.612
  comQ Acinetobacter baylyi ADP1

42.762

100

0.457
  pilQ Acinetobacter baumannii D1279779

40.948

100

0.452
  pilQ Acinetobacter baumannii strain A118

40.948

100

0.452
  pilQ Legionella pneumophila strain ERS1305867

41.429

100

0.414
  pilQ Legionella pneumophila strain Lp02

41.429

100

0.414
  pilQ Vibrio campbellii strain DS40M4

40.941

100

0.414
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.487

99.286

0.412
  pilQ Vibrio cholerae strain A1552

41.487

99.286

0.412