Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC769_RS16720 Genome accession   NZ_CP061672
Coordinates   3558161..3559873 (+) Length   570 a.a.
NCBI ID   WP_191108803.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3553161..3564873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC769_RS16685 (IC769_16685) nusA 3554051..3555535 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  IC769_RS16690 (IC769_16690) rimP 3555573..3556097 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC769_RS16710 (IC769_16710) secG 3556735..3557064 (-) 330 WP_019456770.1 preprotein translocase subunit SecG -
  IC769_RS16715 (IC769_16715) tpiA 3557077..3557871 (-) 795 WP_151749678.1 triose-phosphate isomerase -
  IC769_RS16720 (IC769_16720) pilB 3558161..3559873 (+) 1713 WP_191108803.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC769_RS16725 (IC769_16725) pilC 3559901..3561127 (+) 1227 WP_019456773.1 type II secretion system F family protein Machinery gene
  IC769_RS16730 (IC769_16730) pilD 3561127..3561987 (+) 861 WP_191108804.1 A24 family peptidase Machinery gene
  IC769_RS16735 (IC769_16735) coaE 3561989..3562585 (+) 597 WP_191108805.1 dephospho-CoA kinase -
  IC769_RS16740 (IC769_16740) - 3562582..3563496 (-) 915 WP_191108806.1 DMT family transporter -
  IC769_RS16745 (IC769_16745) rlmB 3563532..3564281 (-) 750 WP_191108807.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC769_RS16750 (IC769_16750) - 3564384..3564710 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63409.69 Da        Isoelectric Point: 5.0842

>NTDB_id=484018 IC769_RS16720 WP_191108803.1 3558161..3559873(+) (pilB) [Acinetobacter seifertii strain AS23]
MSALHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLGQHFAEESNFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFCVFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLQKVIQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=484018 IC769_RS16720 WP_191108803.1 3558161..3559873(+) (pilB) [Acinetobacter seifertii strain AS23]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCTAAGCAGGATACAGTCGCGTATTTAATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTGGCTGAAACAATCTCTGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCAAAAGATTTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCGATCCGCTTTAACAGCAAACTGATGGTTGAGCCTGTCATTG
TCGAGCACCATAAACTAGAAAAAGTTTTAGGTCAGCATTTTGCAGAAGAAAGCAACTTTGACTTTAATGATGAAGAGTTT
GATCTTGATGTCAGTCTTGATGGCCCTACCACTCAAGAGGATGAAGAAGAAACGCCACAAGGTGATGAAGCCCCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGCGCTTCAGACTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTACGTTATCGAGTCGACGGTGTGTTACGTCAAATTGCTAATCCTCCTTTACAACTTGCGAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACATTGTTTGGTGAAAAGTTAGTTTTACGTATTCTTGACCCAT
CAAGTGCGATGCTGGGGATTGACGCATTAGGATACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAGGGCATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAATAGTGC
ACCTGAAACGCTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTGGCACGACGTCTTTGCTCTCAGTGTAAAATACCTACAGATGTTCCAAAACAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGACTTAGCACATCCAGATTTTTGCGTTTTTCAACCGGTAGGTTGTTCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTAGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTCTAGAAA
TCGCGGCCGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTGCAAAAGGTAATACAAGGGGTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

97.544

100

0.975
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.22

93.509

0.479
  pilB Vibrio campbellii strain DS40M4

51.056

91.404

0.467
  pilB Vibrio cholerae strain A1552

50.484

90.702

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

36.833

98.596

0.363