Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IC769_RS16730 Genome accession   NZ_CP061672
Coordinates   3561127..3561987 (+) Length   286 a.a.
NCBI ID   WP_191108804.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3556127..3566987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC769_RS16710 (IC769_16710) secG 3556735..3557064 (-) 330 WP_019456770.1 preprotein translocase subunit SecG -
  IC769_RS16715 (IC769_16715) tpiA 3557077..3557871 (-) 795 WP_151749678.1 triose-phosphate isomerase -
  IC769_RS16720 (IC769_16720) pilB 3558161..3559873 (+) 1713 WP_191108803.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC769_RS16725 (IC769_16725) pilC 3559901..3561127 (+) 1227 WP_019456773.1 type II secretion system F family protein Machinery gene
  IC769_RS16730 (IC769_16730) pilD 3561127..3561987 (+) 861 WP_191108804.1 A24 family peptidase Machinery gene
  IC769_RS16735 (IC769_16735) coaE 3561989..3562585 (+) 597 WP_191108805.1 dephospho-CoA kinase -
  IC769_RS16740 (IC769_16740) - 3562582..3563496 (-) 915 WP_191108806.1 DMT family transporter -
  IC769_RS16745 (IC769_16745) rlmB 3563532..3564281 (-) 750 WP_191108807.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC769_RS16750 (IC769_16750) - 3564384..3564710 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32217.68 Da        Isoelectric Point: 7.1046

>NTDB_id=484020 IC769_RS16730 WP_191108804.1 3561127..3561987(+) (pilD) [Acinetobacter seifertii strain AS23]
MQEIIAYFIQNLTALYVAVALMSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPKCHQS
IRWYQNIPVISWLVLRGKCGDCQHPISIRYPVIELLTMLCSLVVVMVFGPTIQMLWGLVLTWVLITLTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTAPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNENQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=484020 IC769_RS16730 WP_191108804.1 3561127..3561987(+) (pilD) [Acinetobacter seifertii strain AS23]
ATGCAAGAAATCATTGCTTATTTTATTCAAAACCTAACTGCACTTTATGTTGCAGTTGCACTCATGAGCCTATGTATCGG
TAGCTTTCTAAATGTAGTGATTTATCGGACTCCAAAAATGATGGAGCAAGACTGGCAGCAAGAATGTCAAATGCTCCTAA
ATCCAGAGCAACCCATTATTGACCATGAAAAACTGACATTAAGCAAACCTGCTTCATCATGTCCAAAGTGCCATCAATCG
ATTCGTTGGTATCAAAATATTCCTGTCATAAGCTGGCTTGTGCTAAGAGGAAAATGTGGTGATTGCCAACATCCTATCAG
CATTCGTTATCCAGTAATTGAACTACTCACCATGTTATGTTCATTGGTGGTGGTTATGGTGTTTGGCCCGACCATACAAA
TGCTTTGGGGACTCGTCCTGACTTGGGTTTTAATTACCCTCACCTTTATAGACTTTGATACACAACTTTTGCCTGATCGC
TTTACACTGCCTTTAGCAGCACTCGGCTTAGGCATTAACACCTTTAATATTTATACCGCGCCTAACTCAGCTATTTGGGG
TTATTTAATCGGCTTTTTATGCCTATGGATTGTGTATTACTTATTTAAAGTGATTACTGGCAAAGAAGGTATGGGCTACG
GTGACTTTAAATTGCTTGCCGCATTAGGCGCATGGATGGGGCCATTGATGCTGCCATTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATTATTGGCATCATTTTATTAAAGTTAAGAAATGAAAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCGGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAGATTTATTTAGGAGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

95.455

100

0.955
  pilD Acinetobacter baumannii D1279779

95.105

100

0.951
  pilD Vibrio cholerae strain A1552

52.747

95.455

0.503
  pilD Vibrio campbellii strain DS40M4

52.399

94.755

0.497
  pilD Neisseria gonorrhoeae MS11

47.727

92.308

0.441