Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC772_RS17185 Genome accession   NZ_CP061664
Coordinates   3712289..3714001 (+) Length   570 a.a.
NCBI ID   WP_191015937.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS28     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3707289..3719001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC772_RS17150 (IC772_17140) nusA 3708178..3709662 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  IC772_RS17155 (IC772_17145) rimP 3709700..3710224 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC772_RS17175 (IC772_17165) secG 3710862..3711191 (-) 330 WP_002156913.1 preprotein translocase subunit SecG -
  IC772_RS17180 (IC772_17170) tpiA 3711204..3711998 (-) 795 WP_191015935.1 triose-phosphate isomerase -
  IC772_RS17185 (IC772_17175) pilB 3712289..3714001 (+) 1713 WP_191015937.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC772_RS17190 (IC772_17180) pilC 3714029..3715255 (+) 1227 WP_191015938.1 type II secretion system F family protein Machinery gene
  IC772_RS19250 (IC772_17185) pilD 3715255..3716115 (+) 861 WP_191015940.1 prepilin peptidase Machinery gene
  IC772_RS17200 (IC772_17190) coaE 3716117..3716713 (+) 597 WP_191015941.1 dephospho-CoA kinase -
  IC772_RS17205 (IC772_17195) - 3716716..3717625 (-) 910 Protein_3390 DMT family transporter -
  IC772_RS17210 (IC772_17200) rlmB 3717661..3718410 (-) 750 WP_019456777.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC772_RS17215 (IC772_17205) - 3718513..3718839 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63552.88 Da        Isoelectric Point: 5.1618

>NTDB_id=483941 IC772_RS17185 WP_191015937.1 3712289..3714001(+) (pilB) [Acinetobacter seifertii strain AS28]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFNDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483941 IC772_RS17185 WP_191015937.1 3712289..3714001(+) (pilB) [Acinetobacter seifertii strain AS28]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACTAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTGATTG
TCGAGCACCATAAACTCGAAAAAGTTTTAAACCAACATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAAGATGAAGACGAAACACCTCAAGGTGATGAAGCTCCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCAATTCGTATGGGGGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTTGACGGCGTGTTACGTCAAATTGCTAACCCTCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGACCCAT
CAAGTGCGATGCTGGGGATTGACGCATTAGGATACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAGGGCATGCTTTTAATCACAGGTCCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGTTTAAATATTTTAAATAC
TGAGAGCTCTAATATCTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAATCCAA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCTTTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGCCTTTGCTCTCAATGTAAAATACCTACAGATGTTCCGAAACAAAGTCTATTGGAAATGGGCTTT
ACCGAACAGGACCTAGCACATCCGGACTTTCGGGTTTTTCAACCTGTGGGTTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTAGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTCTAGAAA
TCGCGGCCGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTAATGCAAGGGGTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

97.193

100

0.972
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.515

92.632

0.477
  pilB Vibrio cholerae strain A1552

47.88

99.298

0.475
  pilB Vibrio campbellii strain DS40M4

50.288

91.404

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

36.833

98.596

0.363