Detailed information NTG0483914

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC772_RS01700
• Genome accession: NZ_CP061664
• Coordinates: 356156..357214 (+)
• Length: 352 a.a.
• NCBI ID: WP_039251099.1
• Uniprot ID: A0A7H2P6D1
• Organism: Acinetobacter seifertii strain AS28
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 351156..362214

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC772_RS01685 (IC772_01685)	-	352263..352595 (+)	333	WP_077163639.1	hypothetical protein	-
IC772_RS01690 (IC772_01690)	-	352604..353422 (+)	819	WP_410548985.1	putative RNA methyltransferase	-
IC772_RS01695 (IC772_01695)	ponA	353440..355995 (-)	2556	WP_191016390.1	penicillin-binding protein PBP1a	-
IC772_RS01700 (IC772_01700)	pilM	356156..357214 (+)	1059	WP_039251099.1	pilus assembly protein PilM	Machinery gene
IC772_RS01705 (IC772_01705)	pilN	357214..357858 (+)	645	WP_019457875.1	PilN domain-containing protein	Machinery gene
IC772_RS01710 (IC772_01710)	pilO	357855..358595 (+)	741	WP_191016391.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC772_RS01715 (IC772_01715)	pilP	358606..359133 (+)	528	WP_004705193.1	pilus assembly protein PilP	Machinery gene
IC772_RS01720 (IC772_01720)	pilQ	359195..361306 (+)	2112	WP_191016393.1	type IV pilus secretin PilQ family protein	Machinery gene
IC772_RS01725 (IC772_01725)	aroK	361318..361887 (+)	570	WP_191016394.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38994.52 Da
• Isoelectric Point: 4.4622

>NTDB_id=483914 IC772_RS01700 WP_039251099.1 356156..357214(+) (pilM) [Acinetobacter seifertii strain AS28]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483914 IC772_RS01700 WP_039251099.1 356156..357214(+) (pilM) [Acinetobacter seifertii strain AS28]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTACGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACCAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGGCTTG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTGCC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCAGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCTGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATCCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A7H2P6D1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.58	100	0.986
comM	Acinetobacter nosocomialis M2	98.295	100	0.983
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.655	100	0.429