Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IC772_RS01710 Genome accession   NZ_CP061664
Coordinates   357855..358595 (+) Length   246 a.a.
NCBI ID   WP_191016391.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 352855..363595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC772_RS01695 (IC772_01695) ponA 353440..355995 (-) 2556 WP_191016390.1 penicillin-binding protein PBP1a -
  IC772_RS01700 (IC772_01700) pilM 356156..357214 (+) 1059 WP_039251099.1 pilus assembly protein PilM Machinery gene
  IC772_RS01705 (IC772_01705) pilN 357214..357858 (+) 645 WP_019457875.1 PilN domain-containing protein Machinery gene
  IC772_RS01710 (IC772_01710) pilO 357855..358595 (+) 741 WP_191016391.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC772_RS01715 (IC772_01715) pilP 358606..359133 (+) 528 WP_004705193.1 pilus assembly protein PilP Machinery gene
  IC772_RS01720 (IC772_01720) pilQ 359195..361306 (+) 2112 WP_191016393.1 type IV pilus secretin PilQ family protein Machinery gene
  IC772_RS01725 (IC772_01725) aroK 361318..361887 (+) 570 WP_191016394.1 shikimate kinase AroK -
  IC772_RS01730 (IC772_01730) aroB 361916..362998 (+) 1083 WP_191016396.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27829.49 Da        Isoelectric Point: 4.8393

>NTDB_id=483916 IC772_RS01710 WP_191016391.1 357855..358595(+) (pilO) [Acinetobacter seifertii strain AS28]
MSQDELHELSLEQAASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEVFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPTKDSKSDIPVLNYSIKAKTYRYVEATDTAEQSTQAPTPPASTS
TTAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=483916 IC772_RS01710 WP_191016391.1 357855..358595(+) (pilO) [Acinetobacter seifertii strain AS28]
ATGAGTCAAGATGAACTTCACGAGTTATCATTAGAGCAAGCAGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAACAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCCATATCTGTAAAAATTACTTGTTGGATTTTTATTT
TCTTTGCTGTATTAGCCTTGGGTTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCTCAAGCTCAGGAA
AGTAACTTGTTAAACGAATTTCGAGAAAAAGATTCGAAATTACGTAATTTGCAACAGTATCAAGTCCAACTTCAAGAGAT
GCAAGCAAATTTTAATCAGCAATTAGAGCAGTTGCCTAAAGAAACAGAGATTCCTAGTTTGGTTGAGGATATTAACTTAA
CTGGTGTGAACTCAGGATTGAAATTTAAAAATATTCGCTTGGAGAATGAAGTAAAACAGGAAGTCTTTATTGAACAACCG
ATTGCGATAGAAGCCACAGGTGATTATCACGCCTTTGGAGCTTTTGTGAGTAGTATTGCTGCGTTGCCGCGTATCGTAAC
CATGCATGACTTTACTGTTGATGTATCTCCAACTAAAGACAGCAAATCTGATATTCCTGTATTGAATTATTCTATTAAAG
CGAAAACCTATCGCTATGTAGAAGCAACAGATACAGCGGAGCAATCGACCCAAGCTCCAACACCACCTGCTTCAACTAGC
ACGACTGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

91.87

100

0.919
  comO Acinetobacter baylyi ADP1

68.163

99.593

0.679