Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC788_RS16585 Genome accession   NZ_CP061596
Coordinates   3530195..3531907 (+) Length   570 a.a.
NCBI ID   WP_191000794.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS59     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3525195..3536907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC788_RS16550 (IC788_16550) nusA 3526085..3527569 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  IC788_RS16555 (IC788_16555) rimP 3527607..3528131 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC788_RS16575 (IC788_16575) secG 3528769..3529098 (-) 330 WP_019456770.1 preprotein translocase subunit SecG -
  IC788_RS16580 (IC788_16580) tpiA 3529111..3529905 (-) 795 WP_134262172.1 triose-phosphate isomerase -
  IC788_RS16585 (IC788_16585) pilB 3530195..3531907 (+) 1713 WP_191000794.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC788_RS16590 (IC788_16590) pilC 3531935..3533161 (+) 1227 WP_134262170.1 type II secretion system F family protein Machinery gene
  IC788_RS18560 (IC788_16595) pilD 3533161..3534021 (+) 861 WP_134262169.1 prepilin peptidase Machinery gene
  IC788_RS16600 (IC788_16600) coaE 3534023..3534619 (+) 597 WP_049064240.1 dephospho-CoA kinase -
  IC788_RS16605 (IC788_16605) - 3534616..3535530 (-) 915 WP_134262168.1 DMT family transporter -
  IC788_RS16610 (IC788_16610) rlmB 3535562..3536314 (-) 753 WP_191000795.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC788_RS16615 (IC788_16615) - 3536418..3536744 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63476.82 Da        Isoelectric Point: 5.2391

>NTDB_id=483454 IC788_RS16585 WP_191000794.1 3530195..3531907(+) (pilB) [Acinetobacter seifertii strain AS59]
MSALHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLGQHFAEESNFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECRDGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483454 IC788_RS16585 WP_191000794.1 3530195..3531907(+) (pilB) [Acinetobacter seifertii strain AS59]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCTAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTGGCTGAAACAATCTCTGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCGATCCGCTTTAACAGCAAACTGATGGTTGAGCCTGTCATTG
TCGAGCACCATAAACTAGAAAAAGTTTTAGGTCAGCATTTTGCAGAAGAAAGCAACTTTGACTTTAATGATGAAGAGTTT
GATCTTGATGTCAGTCTTGATGGCCCTACCACTCAAGAGGATGAAGAAGAAACGCCACAAGGTGATGAAGCCCCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGCGCTTCAGACTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTACGTTATCGAGTCGACGGTGTGTTACGTCAAATTGCTAATCCTCCTTTGCAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTTCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACATTGTTTGGTGAAAAGTTAGTTTTACGTATTCTTGACCCAT
CAAGTGCGATGCTGGGGATTGACGCATTAGGATACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAGGGCATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCCTTTTTACGACAAGACCCTGACATTATTATGGTCGGTGAAATTCGT
GACTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACCGGTCACATGGTGATGTCAACACTGCACACCAACAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGCCTTTGCTCTCAATGTAAAATACCTACAGATGTTCCAAAACAAAGCCTATTAGAAATGGGCTTT
ACCGAACAGGACTTAGCACATCCAGACTTTCGGGTTTTCCAACCTGTAGGTTGCCCTGAATGTCGTGATGGCTATAAAGG
CCGTGTCGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTAATGCAAGGGGTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

97.895

100

0.979
  pilB Acinetobacter baylyi ADP1

80

100

0.8
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

92.982

0.479
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB Vibrio cholerae strain A1552

50.484

90.702

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365