Detailed information NTG0483421

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC788_RS01500
• Genome accession: NZ_CP061596
• Coordinates: 317145..318203 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS59
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 312145..323203

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC788_RS01485 (IC788_01485)	-	313251..313583 (+)	333	WP_134262507.1	hypothetical protein	-
IC788_RS01490 (IC788_01490)	-	313586..314410 (+)	825	WP_191001028.1	putative RNA methyltransferase	-
IC788_RS01495 (IC788_01495)	ponA	314428..316983 (-)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC788_RS01500 (IC788_01500)	pilM	317145..318203 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC788_RS01505 (IC788_01505)	pilN	318203..318847 (+)	645	WP_077163636.1	PilN domain-containing protein	Machinery gene
IC788_RS01510 (IC788_01510)	pilO	318844..319584 (+)	741	WP_134262501.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC788_RS01515 (IC788_01515)	pilP	319595..320122 (+)	528	WP_004705193.1	pilus assembly protein PilP	Machinery gene
IC788_RS01520 (IC788_01520)	pilQ	320184..322337 (+)	2154	WP_191001029.1	type IV pilus secretin PilQ family protein	Machinery gene
IC788_RS01525 (IC788_01525)	aroK	322349..322918 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483421 IC788_RS01500 WP_004705187.1 317145..318203(+) (pilM) [Acinetobacter seifertii strain AS59]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483421 IC788_RS01500 WP_004705187.1 317145..318203(+) (pilM) [Acinetobacter seifertii strain AS59]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAATTTAGCAAATCCCCAAACTAAAAATGCGGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCTGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGGCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGGGCGAAAAAAGATCGTTCTTTACCAGATGACTATGAAATTGAAGTACTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTACTTGCAGGCG
GAAATGCGAATATTCCAGGTCTCGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426