Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC796_RS17665 Genome accession   NZ_CP061561
Coordinates   3622742..3624454 (+) Length   570 a.a.
NCBI ID   WP_151682978.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS73     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3617742..3629454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC796_RS17630 (IC796_17625) nusA 3618632..3620116 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  IC796_RS17635 (IC796_17630) rimP 3620154..3620678 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC796_RS17655 (IC796_17650) secG 3621316..3621645 (-) 330 WP_191036711.1 preprotein translocase subunit SecG -
  IC796_RS17660 (IC796_17655) tpiA 3621658..3622452 (-) 795 WP_049064232.1 triose-phosphate isomerase -
  IC796_RS17665 (IC796_17660) pilB 3622742..3624454 (+) 1713 WP_151682978.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC796_RS17670 (IC796_17665) pilC 3624482..3625708 (+) 1227 WP_019456773.1 type II secretion system F family protein Machinery gene
  IC796_RS20640 (IC796_17670) pilD 3625708..3626568 (+) 861 WP_151682979.1 prepilin peptidase Machinery gene
  IC796_RS17680 (IC796_17675) coaE 3626570..3627166 (+) 597 WP_151682980.1 dephospho-CoA kinase -
  IC796_RS17685 (IC796_17680) - 3627163..3628077 (-) 915 WP_151682981.1 DMT family transporter -
  IC796_RS17690 (IC796_17685) rlmB 3628113..3628862 (-) 750 WP_151682982.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC796_RS17695 (IC796_17690) - 3628965..3629291 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63443.66 Da        Isoelectric Point: 5.0230

>NTDB_id=483218 IC796_RS17665 WP_151682978.1 3622742..3624454(+) (pilB) [Acinetobacter seifertii strain AS73]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEAHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFNDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFCVFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLQKVIQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483218 IC796_RS17665 WP_151682978.1 3622742..3624454(+) (pilB) [Acinetobacter seifertii strain AS73]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGCGCATCTCTCTC
CTTCTTTACTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTCATTG
TTGAGCACCATAAACTCGAAAAAGTTTTAAACCAGCATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAGGATGAAGACGAAACACCTCAAGGTGATGAAGCACCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGAGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTCGACGGCGTGTTACGTCAAATTGCTAATCCGCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGATCCAT
CAAGCGCAATGCTAGGGATTGACGCCCTAGGATACGAAGAAGAACAAAAAGCTTTGTTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTTGGTGAGATTCGT
GATTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGTCTTTGCTCTCAGTGTAAAATACCTACAGATGTTCCAAAACAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGACTTAGCACATCCAGATTTTTGCGTTTTTCAACCGGTAGGTTGTTCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTAGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTCTAGAAA
TCGCGGCCGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTGCAAAAGGTAATACAAGGGGTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

96.491

100

0.965
  pilB Acinetobacter baylyi ADP1

79.298

100

0.793
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.224

93.158

0.477
  pilB Vibrio cholerae strain A1552

47.88

99.298

0.475
  pilB Vibrio campbellii strain DS40M4

50.669

91.754

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

36.655

98.596

0.361