Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IC796_RS20640 Genome accession   NZ_CP061561
Coordinates   3625708..3626568 (+) Length   286 a.a.
NCBI ID   WP_151682979.1    Uniprot ID   A0A7H2PQF9
Organism   Acinetobacter seifertii strain AS73     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3620708..3631568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC796_RS17655 (IC796_17650) secG 3621316..3621645 (-) 330 WP_191036711.1 preprotein translocase subunit SecG -
  IC796_RS17660 (IC796_17655) tpiA 3621658..3622452 (-) 795 WP_049064232.1 triose-phosphate isomerase -
  IC796_RS17665 (IC796_17660) pilB 3622742..3624454 (+) 1713 WP_151682978.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC796_RS17670 (IC796_17665) pilC 3624482..3625708 (+) 1227 WP_019456773.1 type II secretion system F family protein Machinery gene
  IC796_RS20640 (IC796_17670) pilD 3625708..3626568 (+) 861 WP_151682979.1 prepilin peptidase Machinery gene
  IC796_RS17680 (IC796_17675) coaE 3626570..3627166 (+) 597 WP_151682980.1 dephospho-CoA kinase -
  IC796_RS17685 (IC796_17680) - 3627163..3628077 (-) 915 WP_151682981.1 DMT family transporter -
  IC796_RS17690 (IC796_17685) rlmB 3628113..3628862 (-) 750 WP_151682982.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC796_RS17695 (IC796_17690) - 3628965..3629291 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32167.57 Da        Isoelectric Point: 7.1038

>NTDB_id=483228 IC796_RS20640 WP_151682979.1 3625708..3626568(+) (pilD) [Acinetobacter seifertii strain AS73]
MQEIIAYFIQNLTALYIAVAFVSLCIGSFLNVVIYRTPKMIEQDWQLECQMLLNPEQPIIDHEKLTLSKPASSCPNCHQP
IRWYQNIPIVSWLMLRGKCGYCQHPISIRYPAIELLTMLCSVVVVMVFGPTIQMLWGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPSSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIATAGWVAFLWGDQIMKIYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=483228 IC796_RS20640 WP_151682979.1 3625708..3626568(+) (pilD) [Acinetobacter seifertii strain AS73]
ATGCAAGAAATCATTGCTTATTTTATTCAAAACTTAACTGCTCTTTATATTGCAGTTGCATTCGTGAGCCTATGTATTGG
TAGCTTTCTAAATGTAGTGATTTATCGTACTCCAAAGATGATAGAGCAAGATTGGCAATTAGAATGTCAAATGCTCCTAA
ATCCAGAGCAACCCATTATTGACCATGAAAAACTAACATTAAGCAAACCTGCTTCATCATGTCCAAATTGCCACCAACCG
ATCCGTTGGTATCAAAATATTCCTATCGTAAGCTGGTTGATGCTAAGAGGAAAATGTGGTTATTGCCAACATCCTATCAG
CATTCGTTATCCAGCAATTGAACTACTCACCATGCTATGCTCTGTGGTAGTGGTTATGGTATTTGGCCCAACTATACAAA
TGCTTTGGGGACTCGTTCTTACTTGGGTACTGATTGCCCTTACCTTTATTGATTTCGATACGCAGTTATTACCTGATCGC
TTTACCCTACCTTTGGCTGCACTCGGCTTAGGAATTAACACCTTTAATATTTACACCTCACCTAGCTCAGCCATTTGGGG
ATATTTAATTGGTTTTTTATGTCTTTGGATTGTGTATTACTTATTTAAAGTGATTACTGGCAAAGAAGGTATGGGCTACG
GTGACTTTAAATTGCTTGCCGCATTGGGCGCATGGATGGGGCCATTGATGCTGCCATTAATTGTGTTATTGTCATCTTTA
TTGGGTGCAATTATTGGCATCATTTTATTAAAGTTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAC
TGCGGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAGATTTATTTAGGAGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2PQF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

95.105

100

0.951
  pilD Acinetobacter baumannii D1279779

94.406

100

0.944
  pilD Vibrio cholerae strain A1552

53.383

93.007

0.496
  pilD Vibrio campbellii strain DS40M4

52.652

92.308

0.486
  pilD Neisseria gonorrhoeae MS11

48.106

92.308

0.444