Detailed information NTG0483148

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC797_RS01670
• Genome accession: NZ_CP061556
• Coordinates: 351521..352579 (+)
• Length: 352 a.a.
• NCBI ID: WP_039251099.1
• Uniprot ID: A0A7H2P6D1
• Organism: Acinetobacter seifertii strain AS74
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 346521..357579

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC797_RS01655 (IC797_01655)	-	347628..347960 (+)	333	WP_077163639.1	hypothetical protein	-
IC797_RS01660 (IC797_01660)	-	347963..348787 (+)	825	WP_191105886.1	putative RNA methyltransferase	-
IC797_RS01665 (IC797_01665)	ponA	348805..351359 (-)	2555	Protein_321	penicillin-binding protein PBP1a	-
IC797_RS01670 (IC797_01670)	pilM	351521..352579 (+)	1059	WP_039251099.1	pilus assembly protein PilM	Machinery gene
IC797_RS01675 (IC797_01675)	pilN	352579..353223 (+)	645	WP_077163636.1	PilN domain-containing protein	Machinery gene
IC797_RS01680 (IC797_01680)	pilO	353220..353960 (+)	741	WP_191012245.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC797_RS01685 (IC797_01685)	pilP	353971..354498 (+)	528	WP_004705193.1	pilus assembly protein PilP	Machinery gene
IC797_RS01690 (IC797_01690)	pilQ	354560..356686 (+)	2127	WP_191105887.1	type IV pilus secretin PilQ family protein	Machinery gene
IC797_RS01695 (IC797_01695)	aroK	356698..357267 (+)	570	WP_151682779.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38994.52 Da
• Isoelectric Point: 4.4622

>NTDB_id=483148 IC797_RS01670 WP_039251099.1 351521..352579(+) (pilM) [Acinetobacter seifertii strain AS74]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483148 IC797_RS01670 WP_039251099.1 351521..352579(+) (pilM) [Acinetobacter seifertii strain AS74]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAATTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGTCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACACGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGCGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAG
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCAGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A7H2P6D1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.58	100	0.986
comM	Acinetobacter nosocomialis M2	98.295	100	0.983
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.655	100	0.429