Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IC797_RS01680 Genome accession   NZ_CP061556
Coordinates   353220..353960 (+) Length   246 a.a.
NCBI ID   WP_191012245.1    Uniprot ID   A0A7H2NM85
Organism   Acinetobacter seifertii strain AS74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 348220..358960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC797_RS01665 (IC797_01665) ponA 348805..351359 (-) 2555 Protein_321 penicillin-binding protein PBP1a -
  IC797_RS01670 (IC797_01670) pilM 351521..352579 (+) 1059 WP_039251099.1 pilus assembly protein PilM Machinery gene
  IC797_RS01675 (IC797_01675) pilN 352579..353223 (+) 645 WP_077163636.1 PilN domain-containing protein Machinery gene
  IC797_RS01680 (IC797_01680) pilO 353220..353960 (+) 741 WP_191012245.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC797_RS01685 (IC797_01685) pilP 353971..354498 (+) 528 WP_004705193.1 pilus assembly protein PilP Machinery gene
  IC797_RS01690 (IC797_01690) pilQ 354560..356686 (+) 2127 WP_191105887.1 type IV pilus secretin PilQ family protein Machinery gene
  IC797_RS01695 (IC797_01695) aroK 356698..357267 (+) 570 WP_151682779.1 shikimate kinase AroK -
  IC797_RS01700 (IC797_01700) aroB 357296..358378 (+) 1083 WP_191012243.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27816.67 Da        Isoelectric Point: 5.1461

>NTDB_id=483150 IC797_RS01680 WP_191012245.1 353220..353960(+) (pilO) [Acinetobacter seifertii strain AS74]
MSQDELHELSLEQSASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEVFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVNDSKSDIPVLNYSIKAKTYRYIGTTEAPEKLAKTPVSPAPTS
TNAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=483150 IC797_RS01680 WP_191012245.1 353220..353960(+) (pilO) [Acinetobacter seifertii strain AS74]
ATGAGTCAAGATGAACTTCACGAGTTATCATTAGAGCAATCGGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAACAGTTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCCATATCTGTGAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTATTAGCCTTGGGTTATTTTGTTGCTATTCAGCCAAAGTTACAAGCAATTGATAATGCTCAAGCTCAGGAA
AGTAACTTGTTAAACGAATTTCGAGAAAAAGATTCGAAATTACGTAACTTGCAGCAGTATCAAGTTCAACTTCAGGAGAT
GCAAGCAAATTTTAATCAGCAATTAGAGCAGTTGCCTAAAGAAACCGAGATTCCTAGTTTAGTTGAAGATATCAATTTAA
CGGGTGTGAACTCAGGACTGAAATTTAAAAATATTCGCTTGGAAAATGAAGTAAAACAGGAAGTCTTTATTGAACAACCG
ATTGCGATAGAAGCCACAGGTGATTATCACGCCTTTGGAGCTTTTGTGAGTAGTATTGCTGCGTTGCCGCGTATCGTAAC
CATGCACGACTTTGTTGTAGAGGCTGCCCCTGTTAACGATAGTAAGTCAGATATTCCAGTACTGAATTATTCTATTAAGG
CGAAAACCTATCGTTATATAGGAACAACAGAGGCGCCAGAAAAACTGGCCAAAACGCCAGTATCACCTGCTCCAACTAGC
ACTAATGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2NM85

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

87.398

100

0.874
  comO Acinetobacter baylyi ADP1

74.107

91.057

0.675