Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IAQ69_RS13760 Genome accession   NZ_CP060811
Coordinates   2866180..2867889 (+) Length   569 a.a.
NCBI ID   WP_159122714.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain XM9F202-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2861180..2872889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAQ69_RS13715 (IAQ69_13710) nusA 2861758..2863242 (-) 1485 WP_004787201.1 transcription termination factor NusA -
  IAQ69_RS13720 (IAQ69_13715) rimP 2863278..2863802 (-) 525 WP_179992192.1 ribosome maturation factor RimP -
  IAQ69_RS13750 (IAQ69_13745) secG 2864767..2865096 (-) 330 WP_159122712.1 preprotein translocase subunit SecG -
  IAQ69_RS13755 (IAQ69_13750) tpiA 2865110..2865901 (-) 792 WP_159122713.1 triose-phosphate isomerase -
  IAQ69_RS13760 (IAQ69_13755) pilB 2866180..2867889 (+) 1710 WP_159122714.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IAQ69_RS13765 (IAQ69_13760) pilC 2867920..2869149 (+) 1230 WP_180033708.1 type II secretion system F family protein Machinery gene
  IAQ69_RS13770 (IAQ69_13765) pilD 2869149..2870009 (+) 861 WP_200229182.1 prepilin peptidase Machinery gene
  IAQ69_RS13775 (IAQ69_13770) coaE 2870011..2870616 (+) 606 WP_200229184.1 dephospho-CoA kinase -
  IAQ69_RS13780 (IAQ69_13775) - 2870655..2871572 (-) 918 WP_159122718.1 DMT family transporter -
  IAQ69_RS13785 (IAQ69_13780) rlmB 2871616..2872365 (-) 750 WP_005233526.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IAQ69_RS13790 (IAQ69_13785) - 2872508..2872831 (-) 324 WP_004787221.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63419.51 Da        Isoelectric Point: 4.8353

>NTDB_id=478550 IAQ69_RS13760 WP_159122714.1 2866180..2867889(+) (pilB) [Acinetobacter variabilis strain XM9F202-2]
MTALQGSPRFTGFIRRLVDEGVLSAEDMRTALANAKQEKTDIVAYLVSHHNLSPTTIAETISVEFGEPLFDIGAYDPGQI
LREDIDEKLITKHRILPIYKNANILYVATSNPTNIEATDAIRFATKLNIETVIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVNVDTADPTQEEDEKDKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
EADLQSPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLVKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=478550 IAQ69_RS13760 WP_159122714.1 2866180..2867889(+) (pilB) [Acinetobacter variabilis strain XM9F202-2]
ATGACAGCATTGCAGGGGTCACCAAGATTTACGGGGTTTATTCGTCGCCTGGTCGATGAAGGGGTATTATCAGCTGAAGA
TATGCGGACTGCTTTAGCCAATGCCAAACAGGAAAAAACAGATATCGTGGCCTATTTGGTCAGTCATCATAATCTTTCCC
CTACTACCATTGCTGAAACGATCTCAGTAGAATTTGGTGAGCCTCTATTTGATATTGGTGCTTATGATCCGGGACAAATC
CTGCGTGAAGATATTGATGAAAAGCTGATTACCAAGCATCGTATTCTGCCAATTTATAAAAACGCCAATATTCTTTATGT
CGCCACGAGCAATCCGACCAATATCGAAGCAACGGATGCCATCCGCTTTGCAACCAAACTGAATATTGAAACTGTTATTG
TTGAACACAATAAACTCGAAAAACTGATTGAGCAAAACTTTACTGAAGAAAGTACTTTTGATTTTGATGATGATTTCGAT
CTGGATGTAAATGTCGATACAGCCGATCCGACTCAAGAAGAAGACGAAAAAGACAAGGGTGAAGAAGCCCCTATTGTTAA
ATATATTAATAAGTTACTGATTGATGCTATTCGTATGGGGGCATCAGATTTACATTTTGAACCTTATGAAAAAATGTATC
GGGTGCGCTATCGTGTCGATGGGGTTTTACGTCAGATTGCAACACCGCCGCTACAACTGGCAAACCGTTTGGCGTCGCGT
TTGAAAGTCATGTCGCAGATGGACATTTCTGAGAAACGCGTGCCTCAAGACGGCCGTATTAAATTAAAGCTCTCAAAAAA
CAAAGCCATCGACTTCCGTGTTAACTCGCTCCCTACCCTGTTTGGCGAAAAACTGGTTCTACGTATTTTAGATCCGTCTA
GTGCCATGCTGGGAATTGATGCTTTAGGTTATGAACCTGAGCAGAAAGAATTATTTATGGAAGCCTTAGATAAACCGCAA
GGGATGTTACTGATTACCGGTCCTACTGGTTCCGGTAAAACAGTATCCCTTTATACGGGCTTAAACATCTTAAATCGTGA
AGATACCAATATTTCTACTGCGGAAGACCCAGTCGAGATTAACCTGCAAGGGATTAATCAGGTGAACGTCAATAATAAAG
TTGGCCTGACGTTTGCAGCTGCTTTAAAATCCTTTCTGCGTCAGGATCCTGATATTGTCATGGTCGGTGAGATCCGTGAT
CTGGAAACAGCTGAAATTGCAATCAAAGCAGCGCAAACAGGTCATATGGTGATGTCAACTCTGCACACCAACAGTGCTCC
GGAAACTTTGACCCGTTTACGTAATATGGGCGTACCTTCTTTCAATATCGCGACTTCAGTCAATCTGGTGATTGCGCAGC
GTCTGGCCCGTCGCTTGTGTTCACAATGTAAAAAACCGGCTGACATTCCAAAGCAAAGCTTACTGGAGATGGGCTTTACC
GAAGCAGATTTACAAAGCCCTGAATTTCAGATTTATGAGCCAGTCGGCTGTAATGAATGTCGTGAAGGTTATAAGGGCCG
GGTCGGTATTTATGAAGTTATGAAGGTAACACCTGAGATTTCAAGAATTATTATGGAAGATGGCAATGCCATTGAAATTG
CTGAAGCTTCCGCACGTGCAGGATTTAACAATTTACGCCGATCAGGGTTAGTGAAGGTTATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTAACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.404

100

0.815
  pilB Acinetobacter baylyi ADP1

76.491

100

0.766
  pilB Legionella pneumophila strain ERS1305867

51.59

99.473

0.513
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

49.013

97.891

0.48
  pilB Vibrio campbellii strain DS40M4

48.644

97.188

0.473
  pilB Vibrio cholerae strain A1552

50.677

90.861

0.46
  pilF Thermus thermophilus HB27

38.489

100

0.385
  pilB/pilB1 Synechocystis sp. PCC 6803

34.747

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.319

97.012

0.362