Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7683_RS14425 Genome accession   NZ_CP060288
Coordinates   3090332..3092032 (-) Length   566 a.a.
NCBI ID   WP_209451037.1    Uniprot ID   -
Organism   Pseudomonas mendocina strain CPS5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3085332..3097032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7683_RS14395 (H7683_14390) - 3086087..3086776 (+) 690 WP_209449213.1 energy-coupling factor ABC transporter permease -
  H7683_RS14400 (H7683_14395) - 3086883..3087098 (+) 216 WP_209449214.1 hypothetical protein -
  H7683_RS14405 (H7683_14400) yacG 3087169..3087363 (-) 195 WP_013714026.1 DNA gyrase inhibitor YacG -
  H7683_RS14410 (H7683_14405) coaE 3087360..3087968 (-) 609 WP_209449215.1 dephospho-CoA kinase -
  H7683_RS14415 (H7683_14410) pilD 3088238..3089104 (-) 867 WP_209449216.1 A24 family peptidase Machinery gene
  H7683_RS14420 (H7683_14415) pilC 3089108..3090328 (-) 1221 WP_181563849.1 type II secretion system F family protein Machinery gene
  H7683_RS14425 (H7683_14420) pilB 3090332..3092032 (-) 1701 WP_209451037.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7683_RS24590 (H7683_14425) pilA 3092261..3092446 (+) 186 WP_143501533.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  H7683_RS14435 (H7683_14430) - 3092464..3092892 (+) 429 WP_143501531.1 hypothetical protein -
  H7683_RS14440 (H7683_14435) - 3093046..3093855 (+) 810 WP_244973822.1 hypothetical protein -
  H7683_RS14445 (H7683_14440) - 3093917..3094699 (+) 783 WP_244973823.1 hypothetical protein -
  H7683_RS14450 (H7683_14445) - 3094786..3095607 (+) 822 WP_244973824.1 ABC transporter ATP-binding protein -
  H7683_RS14455 (H7683_14450) nadC 3095715..3096563 (-) 849 WP_209449217.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62211.04 Da        Isoelectric Point: 5.2365

>NTDB_id=475381 H7683_RS14425 WP_209451037.1 3090332..3092032(-) (pilB) [Pseudomonas mendocina strain CPS5]
MNDTAIATGLARQLVNAELLDERAASNAQAQAARNKIPLVSYLVQNKLVSSRALAEVAAEQFGVPYFDLTAIDKESQPRE
VLSEKLCRQHRVLPLYRRGNKLFIALSDPTNHQALTDVQFSAGLSTEALLVEDDKLGDALERFFDTGNTGMEDLADVDLD
GLDVEAVNDDQDDVSAGGDADDAPVVRFINKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGMLHEVARPPIQLASRLSAR
LKVMAQLDISERRKPQDGRIKMKVSKNKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEGQKELYMTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKHGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSCKKEVELPREALLKEGFPE
GQIGTFKLYAPNGCDNCKGGYKGRVGIYEVVKITPSLQRIIMEEGNSIEIAKQARADGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=475381 H7683_RS14425 WP_209451037.1 3090332..3092032(-) (pilB) [Pseudomonas mendocina strain CPS5]
ATGAACGACACCGCGATCGCAACCGGCCTAGCTAGGCAACTCGTCAACGCCGAGCTGCTCGACGAACGTGCAGCCAGCAA
CGCTCAAGCCCAGGCTGCGCGTAACAAGATTCCGCTGGTGTCGTATCTGGTACAGAACAAGCTGGTAAGCAGCCGCGCAC
TGGCAGAGGTAGCAGCCGAGCAATTTGGCGTTCCGTACTTCGACCTGACCGCCATCGACAAGGAGAGCCAACCTCGCGAG
GTGCTGAGCGAAAAGCTCTGCCGGCAGCATCGGGTGCTACCGCTATACCGCCGGGGCAACAAGCTGTTTATCGCCCTCTC
CGACCCGACCAATCACCAGGCGCTGACCGATGTTCAGTTCAGCGCCGGGCTCAGCACCGAGGCGCTACTGGTAGAGGATG
ACAAACTAGGCGATGCCCTGGAGCGTTTTTTCGATACTGGCAACACGGGCATGGAGGATTTAGCCGACGTAGACCTGGAT
GGGCTTGATGTAGAGGCTGTCAACGATGATCAGGACGATGTCAGCGCGGGCGGCGATGCTGATGACGCACCGGTGGTGCG
CTTTATCAACAAGATGCTGCTGGATGCGATCAAGGGCGGCTCATCCGACCTGCACTTCGAACCCTACGAGAAAACCTATC
GCGTGCGTTTTCGTACCGACGGCATGCTGCATGAAGTTGCCCGACCGCCGATTCAGTTGGCTAGTCGCCTCTCTGCGCGC
CTGAAAGTCATGGCGCAACTGGATATATCCGAACGGCGCAAACCGCAAGATGGCCGGATCAAGATGAAGGTATCGAAGAA
CAAGGCCATCGACTTTCGCGTAAACACCCTGCCCACCCTGTGGGGCGAAAAAATCGTGATGCGGATTCTCGACCCTACCA
GCGCCCAAATGGGCATCGATGCCCTGGGTTACGAGGAGGGTCAGAAAGAGCTGTATATGACAGCCCTGAAGCAACCGCAG
GGGATGATTCTGGTAACCGGCCCTACCGGCTCGGGCAAGACGGTATCGCTCTACACCGGGCTGAATATTCTCAATACGCC
GGATATCAATATCTCCACTGCAGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCCAAGC
ATGGCATGGACTTCTCGCAGGCTCTGCGCGCGTTCCTGCGTCAGGATCCAGACGTGATCATGGTAGGCGAGATTCGCGAT
CTGGAAACCGCCGAAATCGCGGTAAAGGCCGCGCAAACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCGGC
GGAAACCCTGACACGTCTACGCAATATGGGCGTACCAGCATTCAATATCGCCACCTCGGTCAACCTGATCATCGCTCAGC
GCCTGGCGCGCAAACTCTGTAGCTGCAAGAAAGAAGTAGAACTGCCGCGCGAGGCTTTACTCAAGGAAGGCTTCCCCGAG
GGCCAGATTGGCACCTTCAAGCTTTACGCTCCCAACGGCTGCGATAATTGCAAAGGCGGCTATAAAGGAAGGGTGGGGAT
TTATGAAGTAGTTAAAATCACGCCCTCGCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGAAATCGCCAAGCAGG
CGCGAGCCGATGGTTTCAACGACCTCCGTACTTCAGGGCTGCTCAAGGCGATGCAGGGCATCACCAGCCTGGAAGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.325

99.47

0.59
  pilB Acinetobacter baylyi ADP1

58.185

99.293

0.578
  pilB Legionella pneumophila strain ERS1305867

54.885

99.47

0.546
  pilB Vibrio cholerae strain A1552

50.617

100

0.507
  pilF Neisseria gonorrhoeae MS11

49.648

100

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.756

95.583

0.495
  pilB Vibrio campbellii strain DS40M4

48.936

99.647

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.286

0.373
  pilF Thermus thermophilus HB27

39.623

93.64

0.371