Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUU81_RS02620 Genome accession   NZ_CP059888
Coordinates   593858..595546 (-) Length   562 a.a.
NCBI ID   WP_199610728.1    Uniprot ID   -
Organism   Flocculibacter collagenilyticus strain SM1988     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 588858..600546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU81_RS02595 yacG 589834..590094 (-) 261 WP_199610723.1 DNA gyrase inhibitor YacG -
  HUU81_RS02600 zapD 590173..590919 (-) 747 WP_199610724.1 cell division protein ZapD -
  HUU81_RS02605 coaE 591026..591649 (-) 624 WP_199610725.1 dephospho-CoA kinase -
  HUU81_RS02610 pilD 591677..592570 (-) 894 WP_407644844.1 prepilin peptidase Machinery gene
  HUU81_RS02615 pilC 592577..593803 (-) 1227 WP_199610727.1 type II secretion system F family protein Machinery gene
  HUU81_RS02620 pilB 593858..595546 (-) 1689 WP_199610728.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUU81_RS17550 - 595684..596142 (-) 459 WP_407644831.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HUU81_RS02630 nadC 596836..597681 (-) 846 WP_199610729.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUU81_RS02635 - 597704..598219 (-) 516 WP_199610730.1 TIGR02281 family clan AA aspartic protease -
  HUU81_RS02640 rph 598481..599194 (+) 714 WP_199610731.1 ribonuclease PH -
  HUU81_RS02645 pyrE 599382..600026 (+) 645 WP_199610732.1 orotate phosphoribosyltransferase -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62395.21 Da        Isoelectric Point: 4.9601

>NTDB_id=472366 HUU81_RS02620 WP_199610728.1 593858..595546(-) (pilB) [Flocculibacter collagenilyticus strain SM1988]
MRFSTASPLARRLIAANITSEEHLAEKANKQSHYHNLTDFLVSENFISSENLAKFCCTCFGTSYLDINLFDLTSIDDELK
NQSLSKKHHAIPLKKHNRTLMLAMSDPTDIRAIEDFEFNTGLQVECVVAEIEYIKLALESIYDSHEELDVSEVDFKELGE
LEVEEDTDEDDSSDSSDAPIIVYINKILMDAIRKGASDLHFEPYEKAYRIRFRIDGVLKEVAHPPVALANKLSARIKVMS
RLDIAEKRLPQDGRIKLKITKNKSIDFRVSSLPTLWGEKIVMRILDSSSAQLGIDVLGYEEAQKQLYLDALAQPQGMILV
TGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVQINTKAGLDFASALRSFLRQDPDIVMVGEIRDLETAE
IAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVTLIIAQRLARRLCPECKEPEQLPKQTLLDTGFTEQQMP
HIQLFKPIGCEKCTDGYKGRVGIYETMPITPNISKIIMEGGNSLDIANQAAQEGINNLRKSGLLKAANGITSLSEINRVT
SF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=472366 HUU81_RS02620 WP_199610728.1 593858..595546(-) (pilB) [Flocculibacter collagenilyticus strain SM1988]
ATGAGATTTAGTACTGCGTCTCCTCTTGCAAGGAGACTTATTGCTGCAAACATAACCTCTGAAGAACACTTAGCAGAAAA
AGCGAATAAGCAATCACACTATCATAATCTTACAGATTTTCTAGTATCAGAAAATTTCATTTCAAGTGAAAACCTTGCAA
AATTTTGCTGCACTTGCTTTGGCACATCTTATCTAGACATAAACTTATTCGACTTAACAAGCATTGATGATGAACTGAAA
AATCAATCTTTAAGCAAAAAACATCACGCTATCCCACTAAAAAAGCATAATAGAACTTTAATGCTTGCAATGTCAGATCC
CACTGACATACGTGCTATTGAAGATTTTGAATTTAATACAGGCCTACAAGTAGAGTGTGTTGTAGCAGAAATTGAATATA
TAAAACTAGCGCTCGAATCTATATATGATAGCCATGAAGAACTCGACGTATCTGAGGTCGATTTTAAAGAGCTTGGGGAA
TTGGAAGTTGAAGAAGACACTGATGAAGATGACTCATCAGATTCAAGTGACGCCCCTATTATCGTCTACATTAATAAGAT
ATTGATGGATGCAATCCGTAAAGGTGCATCGGATCTACACTTTGAGCCTTATGAAAAAGCTTATCGAATTCGTTTTCGTA
TAGATGGTGTTTTGAAAGAAGTAGCCCACCCTCCAGTAGCTCTTGCTAATAAATTATCTGCGCGTATTAAAGTAATGTCT
CGGTTAGATATTGCTGAAAAACGTTTACCGCAAGACGGCCGTATAAAGCTAAAAATTACTAAAAATAAATCTATCGATTT
TCGTGTTTCATCACTTCCTACATTGTGGGGAGAGAAAATTGTAATGCGTATTCTTGACTCATCCAGTGCGCAACTTGGTA
TTGATGTTTTAGGTTATGAAGAAGCACAAAAACAGCTATATTTAGATGCGCTAGCACAACCTCAAGGTATGATTCTAGTT
ACAGGCCCGACAGGTTCAGGTAAAACGGTATCACTTTATACTGGCTTAAACATTTTAAATACAGAAGAACGCAATATTTC
TACGGCGGAAGACCCAGTTGAAATAAACTTAGAAGGTGTTAATCAGGTTCAAATTAACACGAAAGCAGGTTTAGACTTTG
CGTCGGCTTTACGCTCTTTTCTTCGACAAGATCCCGACATAGTAATGGTAGGTGAAATCCGTGATTTAGAAACAGCGGAA
ATTGCAATAAAAGCAGCACAAACGGGCCACTTAGTTTTATCTACTTTGCATACTAATTCAGCACCAGAAACGTTAACGCG
CCTGTTAAATATGGGCGTACCTGCCTACAATGTTGCCAGCTCAGTAACTTTAATTATTGCTCAACGACTTGCAAGAAGAT
TATGTCCCGAATGTAAAGAGCCAGAACAGCTCCCAAAACAAACCCTACTTGACACAGGCTTTACAGAGCAACAAATGCCA
CATATACAATTATTTAAGCCCATTGGCTGTGAGAAATGTACAGATGGGTATAAGGGTCGTGTAGGTATTTATGAAACCAT
GCCAATCACACCTAATATTTCCAAAATCATTATGGAGGGAGGAAATTCATTGGATATAGCTAATCAAGCAGCTCAAGAAG
GCATAAATAATTTAAGAAAATCTGGTCTTTTAAAAGCGGCCAATGGTATAACTAGCCTATCTGAAATTAACCGTGTAACT
AGCTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-147)

Type II/IV secretion system protein (T2SSE)

(186-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.661

100

0.568
  pilB Acinetobacter baumannii D1279779

59.924

94.128

0.564
  pilB Vibrio parahaemolyticus RIMD 2210633

53.71

100

0.541
  pilB Vibrio campbellii strain DS40M4

52.717

98.221

0.518
  pilB Legionella pneumophila strain ERS1305867

54.323

94.662

0.514
  pilB Vibrio cholerae strain A1552

52.345

94.84

0.496
  pilF Neisseria gonorrhoeae MS11

48.732

98.221

0.479
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.667

100

0.372
  pilF Thermus thermophilus HB27

37.03

99.466

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

39.231

92.527

0.363