Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   H3302_RS13180 Genome accession   NZ_CP059831
Coordinates   2914017..2915246 (+) Length   409 a.a.
NCBI ID   WP_182395472.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. MT33b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2909017..2920246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3302_RS13155 (H3302_13155) ampE 2909259..2910110 (-) 852 WP_036965921.1 beta-lactamase regulator AmpE -
  H3302_RS13160 (H3302_13160) ampD 2910134..2910676 (-) 543 WP_180311088.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H3302_RS13165 (H3302_13165) nadC 2910866..2911714 (+) 849 WP_074988951.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H3302_RS13170 (H3302_13170) - 2912212..2912655 (+) 444 WP_180311090.1 pilin -
  H3302_RS13175 (H3302_13175) - 2912702..2913997 (+) 1296 WP_180311091.1 O-antigen ligase family protein -
  H3302_RS13180 (H3302_13180) pilC 2914017..2915246 (+) 1230 WP_182395472.1 type II secretion system F family protein Machinery gene
  H3302_RS13185 (H3302_13185) pilD 2915426..2916322 (+) 897 WP_180311093.1 A24 family peptidase Machinery gene
  H3302_RS13190 (H3302_13190) coaE 2916325..2916933 (+) 609 WP_180311094.1 dephospho-CoA kinase -
  H3302_RS13195 (H3302_13195) pilB 2917096..2918772 (+) 1677 WP_118844625.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3302_RS13200 (H3302_13200) yacG 2918840..2919073 (+) 234 WP_036965896.1 DNA gyrase inhibitor YacG -
  H3302_RS13205 (H3302_13205) - 2919135..2919665 (-) 531 WP_051501715.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44614.93 Da        Isoelectric Point: 9.7944

>NTDB_id=472125 H3302_RS13180 WP_182395472.1 2914017..2915246(+) (pilC) [Pseudoalteromonas sp. MT33b]
MSKQIEKNKLDTFQWVGLSARGKKLEGELSGQSIALVKAQLRKQGITPSKVKRKAKPLFGIKHVQKIPPKDIALITRQIA
TMLMAGVPLIQSIEMIGSGAKNKSVARLMEEIGDEVKAGVPLSQALRKHPRYFDDLYCDLVASGEQSGALDKIFDRVALY
KEKAEALKSKIKKAMFYPIAVLVVALIVTSILLIFVVPQFQEIFNGFGAELPAFTLMVIGISEFMQEYWWIMLIAVIAFG
YAYKEALLKSKKLRDANDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILEIKAEV
SSGNLMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=472125 H3302_RS13180 WP_182395472.1 2914017..2915246(+) (pilC) [Pseudoalteromonas sp. MT33b]
ATGTCAAAACAAATAGAAAAAAATAAATTAGATACTTTTCAATGGGTTGGTTTAAGTGCCAGAGGAAAGAAACTTGAAGG
TGAACTTTCTGGTCAGAGTATAGCATTGGTCAAAGCACAGCTCCGTAAACAAGGAATTACTCCTTCTAAAGTTAAGCGCA
AAGCAAAGCCATTATTTGGCATTAAACATGTACAAAAAATCCCCCCTAAAGACATTGCCCTGATTACACGCCAAATAGCG
ACCATGCTGATGGCTGGTGTGCCGCTTATTCAATCTATTGAAATGATAGGTTCAGGTGCTAAAAATAAAAGTGTTGCTAG
GCTGATGGAAGAAATTGGTGACGAAGTAAAAGCGGGTGTGCCACTCTCGCAAGCTTTACGCAAACACCCCCGTTATTTTG
ATGATTTATATTGCGACCTTGTTGCATCGGGTGAACAATCGGGTGCCCTTGATAAAATTTTCGACCGTGTCGCTCTTTAT
AAAGAAAAAGCCGAAGCATTAAAATCTAAAATAAAAAAAGCAATGTTTTACCCGATTGCGGTATTAGTTGTGGCCTTAAT
CGTTACCTCAATCTTACTTATTTTTGTGGTGCCTCAATTCCAAGAGATTTTTAACGGCTTTGGTGCTGAGCTTCCCGCCT
TTACTTTAATGGTGATAGGTATATCTGAATTTATGCAAGAATATTGGTGGATAATGCTGATTGCTGTTATTGCATTTGGC
TATGCCTATAAAGAAGCGCTGCTTAAAAGCAAAAAACTCAGAGATGCCAACGACAGAGCTATATTAAAGTTACCTGTTAT
TGGCATGATTTTAAATAAAGCAGCAGTGGCTCGTTATGCACGCACTCTTTCAACAACCTTTGCTGCGGGTGTGCCCTTGG
TTGATGCGCTCGACTCCGCCGCGGGAGCCTCAGGTAATGCAGTGTACCGTTACGCTATTTTAGAGATAAAAGCAGAAGTG
AGCTCAGGTAATTTGATGAACTGGGCAATGCGAAACTCTAAAATATTTCCTGATATGGTGATCCAAATGGTCGCAATTGG
TGAAGAGTCAGGCTCATTAGATGGCATGCTTGCTAAAGTAGCCACTATTTATGAGCAAGAGGTTGATGACGCGGTCGATG
GCCTTTCGAGCTTATTGGAGCCGTTGATCATGGCTGTACTTGGTGTGCTAGTCGGTGGTTTAATCGTTGCTATGTATTTA
CCTATTTTCCAGTTAGGTTCCGTCATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.858

100

0.619
  pilC Legionella pneumophila strain ERS1305867

54.791

99.511

0.545
  pilC Acinetobacter baumannii D1279779

55

97.8

0.538
  pilC Acinetobacter baylyi ADP1

54.408

97.066

0.528
  pilC Vibrio cholerae strain A1552

45.161

98.533

0.445
  pilG Neisseria meningitidis 44/76-A

43.909

96.333

0.423
  pilC Vibrio campbellii strain DS40M4

43.142

98.044

0.423
  pilG Neisseria gonorrhoeae MS11

43.655

96.333

0.421