NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	H2513_RS05720	Genome accession	NZ_CP059704
Coordinates	1247275..1247709 (-)	Length	144 a.a.
NCBI ID	WP_316729321.1	Uniprot ID	A0A345H7N5
Organism	Pasteurella multocida strain RCAD0730
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1242275..1252709

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2513_RS05690 (H2513_05670)	-	1242836..1243105 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
H2513_RS05695 (H2513_05675)	yacG	1243105..1243308 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
H2513_RS05700 (H2513_05680)	coaE	1243319..1243939 (-)	621	WP_005751078.1	dephospho-CoA kinase	-
H2513_RS05705 (H2513_05685)	-	1243954..1244676 (-)	723	WP_316729318.1	A24 family peptidase	-
H2513_RS05710 (H2513_05690)	pilC	1244676..1245887 (-)	1212	WP_316730679.1	type II secretion system F family protein	Machinery gene
H2513_RS05715 (H2513_05695)	pilB	1245887..1247275 (-)	1389	WP_316729319.1	GspE/PulE family protein	Machinery gene
H2513_RS05720 (H2513_05700)	pilA	1247275..1247709 (-)	435	WP_316729321.1	prepilin peptidase-dependent pilin	Machinery gene
H2513_RS05725 (H2513_05705)	ampD	1247869..1248435 (+)	567	WP_005751074.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
H2513_RS05730 (H2513_05710)	rppH	1249020..1249616 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
H2513_RS05735 (H2513_05715)	-	1249619..1250413 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
H2513_RS05740 (H2513_05720)	lgt	1250423..1251235 (+)	813	WP_156732338.1	prolipoprotein diacylglyceryl transferase	-
H2513_RS05745 (H2513_05725)	-	1251232..1252083 (+)	852	WP_005751072.1	thymidylate synthase	-
H2513_RS05750 (H2513_05730)	tadA	1252098..1252598 (+)	501	WP_005753343.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15086.53 Da Isoelectric Point: 9.1281

                                >NTDB_id=471470 H2513_RS05720 WP_316729321.1 1247275..1247709(-) (pilA) [Pasteurella multocida strain RCAD0730]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAVAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=471470 H2513_RS05720 WP_316729321.1 1247275..1247709(-) (pilA) [Pasteurella multocida strain RCAD0730]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGTTGCTC

CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A345H7N5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.735	100	0.375
pilA	Vibrio cholerae strain A1552	36.735	100	0.375
pilA	Vibrio cholerae C6706	36.735	100	0.375

Detailed information NTG0471470