Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H2513_RS05715 Genome accession   NZ_CP059704
Coordinates   1245887..1247275 (-) Length   462 a.a.
NCBI ID   WP_316729319.1    Uniprot ID   -
Organism   Pasteurella multocida strain RCAD0730     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1240887..1252275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2513_RS05685 (H2513_05665) - 1241014..1242387 (-) 1374 WP_169029464.1 sodium:alanine symporter family protein -
  H2513_RS05690 (H2513_05670) - 1242836..1243105 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  H2513_RS05695 (H2513_05675) yacG 1243105..1243308 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  H2513_RS05700 (H2513_05680) coaE 1243319..1243939 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  H2513_RS05705 (H2513_05685) - 1243954..1244676 (-) 723 WP_316729318.1 A24 family peptidase -
  H2513_RS05710 (H2513_05690) pilC 1244676..1245887 (-) 1212 WP_316730679.1 type II secretion system F family protein Machinery gene
  H2513_RS05715 (H2513_05695) pilB 1245887..1247275 (-) 1389 WP_316729319.1 GspE/PulE family protein Machinery gene
  H2513_RS05720 (H2513_05700) pilA 1247275..1247709 (-) 435 WP_316729321.1 prepilin peptidase-dependent pilin Machinery gene
  H2513_RS05725 (H2513_05705) ampD 1247869..1248435 (+) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H2513_RS05730 (H2513_05710) rppH 1249020..1249616 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  H2513_RS05735 (H2513_05715) - 1249619..1250413 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  H2513_RS05740 (H2513_05720) lgt 1250423..1251235 (+) 813 WP_156732338.1 prolipoprotein diacylglyceryl transferase -
  H2513_RS05745 (H2513_05725) - 1251232..1252083 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52228.94 Da        Isoelectric Point: 6.4990

>NTDB_id=471469 H2513_RS05715 WP_316729319.1 1245887..1247275(-) (pilB) [Pasteurella multocida strain RCAD0730]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPPFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCR
VEAQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=471469 H2513_RS05715 WP_316729319.1 1245887..1247275(-) (pilB) [Pasteurella multocida strain RCAD0730]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCCCTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGTCGT
GTAGAGGCACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.052

0.669
  pilB Haemophilus influenzae Rd KW20

67.55

98.052

0.662
  pilB Glaesserella parasuis strain SC1401

58.499

98.052

0.574
  pilB Acinetobacter baumannii D1279779

39.596

100

0.424
  pilB Vibrio parahaemolyticus RIMD 2210633

39.752

100

0.416
  pilB Vibrio cholerae strain A1552

39.425

100

0.416
  pilB Acinetobacter baylyi ADP1

38.124

100

0.413
  pilB Vibrio campbellii strain DS40M4

39.583

100

0.411
  pilB Legionella pneumophila strain ERS1305867

37.678

100

0.4