Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   H3L93_RS07715 Genome accession   NZ_CP059569
Coordinates   1430613..1431896 (-) Length   427 a.a.
NCBI ID   WP_003794498.1    Uniprot ID   C4GF88
Organism   Kingella oralis strain DSM 18271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1425613..1436896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L93_RS07685 (H3L93_07665) - 1426654..1427100 (+) 447 WP_003794510.1 META domain-containing protein -
  H3L93_RS07690 (H3L93_07670) - 1427174..1427794 (-) 621 WP_003794508.1 L-threonylcarbamoyladenylate synthase -
  H3L93_RS07695 (H3L93_07675) pncC 1427854..1428330 (-) 477 WP_003794506.1 nicotinamide-nucleotide amidase -
  H3L93_RS07700 (H3L93_07680) zapD 1428357..1429118 (-) 762 WP_003794504.1 cell division protein ZapD -
  H3L93_RS07705 (H3L93_07685) coaE 1429159..1429767 (-) 609 WP_003794503.1 dephospho-CoA kinase -
  H3L93_RS07710 (H3L93_07690) pilD 1429769..1430602 (-) 834 WP_040558143.1 A24 family peptidase Machinery gene
  H3L93_RS07715 (H3L93_07695) pilG 1430613..1431896 (-) 1284 WP_003794498.1 type II secretion system F family protein Machinery gene
  H3L93_RS07720 (H3L93_07700) pilF 1431920..1433605 (-) 1686 WP_003794495.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L93_RS07735 (H3L93_07715) lolD 1434079..1434768 (-) 690 WP_003794493.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  H3L93_RS07740 (H3L93_07720) - 1434761..1436023 (-) 1263 WP_003794491.1 lipoprotein-releasing ABC transporter permease subunit -

Sequence

Protein


Download         Length: 427 a.a.        Molecular weight: 46453.94 Da        Isoelectric Point: 10.1356

>NTDB_id=470798 H3L93_RS07715 WP_003794498.1 1430613..1431896(-) (pilG) [Kingella oralis strain DSM 18271]
MASLAKPAKKAAPKKLVAKKPAANVKKVEKGNRYQFEGKSLQSDSIVRGEVVAKTEDEARQKLARRQIKIIQLTKMKKQR
QKKITSADIAVFTRQLSTMMKAGLPLMQAFDIVAKGHSNAAMTNLLLDVKNSVEQGNSLASSFSQHPKYFDKFYCNLVAA
GEAGGVLEGLLDKLATYMEKTQAIKKKVKSALTYPIAVIVVAIVLILVMMMFVLPSFKKIYEGLGAELPGLTQWMMNLSD
FFVTQGWIIIPILIGAIVGLVQFHKRSPAFQKRVDGWLLKMPIFGEIVRKATVARWARTTATLFTAGVPLVEVLDSVAGA
SGNIIYEEATYEIRGKVNQGISLTSGMQASGVFPNMVVQMASIGEESGALDDMLNKVAEFYEDEVDAAVATLSSLMEPII
MVVLGGIVGVILIAMYLPLFSIGDAIG

Nucleotide


Download         Length: 1284 bp        

>NTDB_id=470798 H3L93_RS07715 WP_003794498.1 1430613..1431896(-) (pilG) [Kingella oralis strain DSM 18271]
ATGGCATCTCTAGCAAAACCTGCTAAAAAAGCAGCTCCTAAGAAATTAGTAGCAAAAAAGCCTGCTGCAAATGTTAAGAA
AGTAGAAAAAGGCAATCGCTATCAGTTTGAAGGAAAAAGTCTACAAAGTGATTCGATTGTGCGCGGTGAGGTGGTGGCGA
AAACAGAAGATGAAGCACGTCAGAAATTAGCCCGCCGCCAAATCAAAATCATCCAACTGACAAAAATGAAAAAACAACGC
CAGAAGAAGATTACTTCTGCGGATATTGCAGTGTTTACACGTCAGTTATCTACTATGATGAAAGCGGGTTTGCCGCTGAT
GCAAGCATTTGATATTGTGGCGAAAGGTCATTCTAATGCAGCAATGACCAATTTGCTGCTGGATGTAAAAAATAGCGTTG
AGCAAGGTAATTCCTTGGCGAGTTCTTTTTCACAACATCCTAAATATTTTGATAAATTTTATTGCAACTTGGTTGCTGCT
GGCGAAGCGGGTGGTGTATTGGAAGGTTTGCTAGATAAATTAGCAACCTATATGGAAAAAACGCAAGCCATTAAGAAAAA
AGTAAAAAGTGCGCTCACTTATCCGATTGCTGTGATTGTGGTGGCGATTGTTTTAATCTTGGTCATGATGATGTTTGTAT
TGCCATCCTTTAAGAAAATTTATGAGGGACTTGGTGCAGAATTACCAGGTTTGACGCAATGGATGATGAATTTGTCTGAT
TTTTTTGTAACTCAAGGATGGATAATTATTCCAATATTGATTGGTGCGATAGTTGGCTTAGTTCAATTTCATAAACGTTC
TCCTGCATTCCAAAAACGAGTAGATGGATGGTTATTAAAAATGCCGATTTTTGGTGAAATTGTACGCAAAGCAACAGTTG
CACGTTGGGCGCGTACAACAGCAACTCTGTTTACTGCGGGTGTGCCGTTGGTTGAAGTGTTGGATTCTGTTGCGGGCGCA
TCAGGCAATATTATCTACGAAGAAGCTACTTATGAAATCCGCGGTAAAGTAAATCAAGGTATTTCGCTCACATCTGGTAT
GCAAGCATCGGGCGTTTTTCCTAACATGGTGGTGCAGATGGCATCCATTGGTGAAGAATCAGGTGCGCTGGACGATATGT
TAAATAAAGTGGCTGAATTCTATGAAGATGAGGTGGATGCGGCAGTAGCGACACTTTCATCCTTAATGGAACCCATCATC
ATGGTTGTTTTAGGCGGTATCGTAGGTGTAATTTTGATTGCGATGTATCTGCCATTATTCAGCATTGGTGATGCAATCGG
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(296-418)

Type II secretion system (T2SS), protein F (T2SSF)

(92-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4GF88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

64.322

93.208

0.6
  pilG Neisseria meningitidis 44/76-A

63.568

93.208

0.593
  pilC Legionella pneumophila strain ERS1305867

47.396

89.93

0.426
  pilC Acinetobacter baumannii D1279779

44.226

95.316

0.422
  pilC Acinetobacter baylyi ADP1

45.974

90.164

0.415
  pilC Pseudomonas stutzeri DSM 10701

50.435

80.796

0.407