Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H3L93_RS07720 Genome accession   NZ_CP059569
Coordinates   1431920..1433605 (-) Length   561 a.a.
NCBI ID   WP_003794495.1    Uniprot ID   C4GF87
Organism   Kingella oralis strain DSM 18271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1426920..1438605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L93_RS07690 (H3L93_07670) - 1427174..1427794 (-) 621 WP_003794508.1 L-threonylcarbamoyladenylate synthase -
  H3L93_RS07695 (H3L93_07675) pncC 1427854..1428330 (-) 477 WP_003794506.1 nicotinamide-nucleotide amidase -
  H3L93_RS07700 (H3L93_07680) zapD 1428357..1429118 (-) 762 WP_003794504.1 cell division protein ZapD -
  H3L93_RS07705 (H3L93_07685) coaE 1429159..1429767 (-) 609 WP_003794503.1 dephospho-CoA kinase -
  H3L93_RS07710 (H3L93_07690) pilD 1429769..1430602 (-) 834 WP_040558143.1 A24 family peptidase Machinery gene
  H3L93_RS07715 (H3L93_07695) pilG 1430613..1431896 (-) 1284 WP_003794498.1 type II secretion system F family protein Machinery gene
  H3L93_RS07720 (H3L93_07700) pilF 1431920..1433605 (-) 1686 WP_003794495.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L93_RS07735 (H3L93_07715) lolD 1434079..1434768 (-) 690 WP_003794493.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  H3L93_RS07740 (H3L93_07720) - 1434761..1436023 (-) 1263 WP_003794491.1 lipoprotein-releasing ABC transporter permease subunit -
  H3L93_RS07745 (H3L93_07725) - 1436133..1437035 (+) 903 WP_003794489.1 DNA repair protein RecO -
  H3L93_RS07750 (H3L93_07730) - 1437210..1437350 (-) 141 WP_003794487.1 entericidin A/B family lipoprotein -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62370.32 Da        Isoelectric Point: 5.0583

>NTDB_id=470799 H3L93_RS07720 WP_003794495.1 1431920..1433605(-) (pilF) [Kingella oralis strain DSM 18271]
MSIGLLRILVQQKLVPQENIEKYQAAVKAEKPVMPLLFGDKIITPEALAELCARLFNYPVLDLNYYPRSRVIKDVMTSEQ
MKEFRCIPLLKRGRSLYVGVSDPTQIQNVQKLAFASGLQVELVVVRDDQLTNVLDWFGQSNNELLKEMDVAATQLENGQA
PAMIVDGVEEEDGPVARFIQKILADAVQSGASDIHFEFYEFMARVRFRADGQLREVVQPPLSVRNQLASRIKVMAKMDIS
EKRVPQDGRIQLQFSKNAKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEDFQKELIMEAINRPYGMVLVTGPT
GSGKTVSLYTCLNILNTEDINISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDVIMIGEIRDLGTADIAIK
AAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCVACKQPMERPPKEALLDVGFTEEDLAKEW
TMYRPVGCDACKGKGYKGRAGLYEVMPMTEKMKEVIMKGGTEVDIANIAYEEGLVDLRRSGLLKVMQGITSLEEVIATTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=470799 H3L93_RS07720 WP_003794495.1 1431920..1433605(-) (pilF) [Kingella oralis strain DSM 18271]
ATGAGTATTGGTTTATTGCGTATTTTGGTACAGCAAAAACTTGTTCCGCAAGAGAATATTGAAAAATATCAAGCAGCCGT
AAAAGCAGAAAAACCCGTTATGCCCTTGCTTTTTGGCGACAAAATCATCACGCCAGAGGCGTTGGCAGAATTGTGCGCCC
GCTTGTTTAATTACCCTGTTTTGGATTTGAATTACTATCCGCGCAGCCGCGTAATTAAAGACGTGATGACATCGGAACAA
ATGAAAGAATTTCGTTGCATCCCATTGTTGAAGCGCGGACGTTCTTTGTATGTGGGCGTTTCCGACCCCACGCAAATCCA
AAATGTGCAAAAACTGGCGTTTGCATCAGGCTTGCAAGTTGAGCTGGTGGTGGTGCGCGATGACCAATTAACCAATGTGT
TGGACTGGTTTGGTCAAAGCAACAACGAATTGTTAAAAGAAATGGACGTTGCTGCCACGCAACTGGAAAACGGTCAAGCT
CCCGCCATGATTGTGGATGGCGTGGAAGAAGAAGATGGGCCTGTTGCGCGGTTTATTCAAAAAATTCTGGCCGATGCGGT
TCAATCAGGCGCATCCGATATTCACTTTGAATTTTACGAATTTATGGCGCGTGTTCGCTTTCGTGCCGACGGGCAGTTGC
GCGAGGTGGTGCAACCACCGCTTTCTGTGCGCAACCAGCTGGCATCGCGTATCAAAGTGATGGCAAAAATGGATATTTCC
GAAAAGCGTGTTCCACAAGACGGTCGTATCCAATTGCAATTTTCCAAAAACGCCAAAGCCATTGACTTTCGTGTGAACAC
CATGCCCTGCTTGTTTGGTGAAAAAGTGGTGATGCGTATTTTGAACTCAGACGCGGCTAGCTTGAATATTGACCAATTGG
GCTTTGAAGACTTCCAAAAAGAACTGATTATGGAAGCCATCAATCGCCCATACGGCATGGTGTTGGTAACAGGACCAACA
GGTTCGGGTAAGACGGTATCGCTTTATACTTGCTTAAATATTCTCAATACCGAAGATATCAATATTTCCACCGCAGAAGA
CCCCGCCGAGATTAACTTACCAGGGATTAACCAAGTAAACGTAAATGATAAGCAAGGCTTAACGTTTGAAGCGGCACTAA
AAGCATTCCTGCGGCAAGACCCGGATGTTATCATGATTGGTGAGATTCGTGATTTGGGTACGGCGGATATTGCGATTAAA
GCGGCACAAACGGGGCACATGGTGTTCTCAACCTTGCATACCAATAATGCACCCGCAACACTATCTCGTATGCTCAATAT
GGGAGTTGCGCCTTTTAATATTGCCAGTGCGGTAAACTTGATTATGGCGCAACGCTTGGCGCGTCGTTTGTGCGTGGCGT
GTAAACAACCGATGGAGCGTCCGCCCAAAGAAGCATTATTGGATGTGGGGTTTACGGAAGAAGATTTGGCAAAAGAATGG
ACTATGTATCGCCCTGTTGGTTGCGATGCTTGCAAAGGCAAGGGTTATAAAGGGCGTGCTGGTTTATATGAAGTGATGCC
GATGACCGAAAAAATGAAAGAAGTCATTATGAAGGGCGGTACGGAAGTAGATATTGCCAACATTGCCTACGAAGAAGGAT
TGGTGGATTTGCGTCGTTCTGGATTGTTGAAAGTTATGCAAGGCATTACTTCGTTAGAGGAAGTAATCGCAACAACAAAT
GAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(181-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4GF87

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.162

100

0.692
  pilB Acinetobacter baumannii D1279779

52.548

100

0.533
  pilB Acinetobacter baylyi ADP1

51.852

100

0.524
  pilB Legionella pneumophila strain ERS1305867

46.964

99.822

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

45.884

100

0.467
  pilB Vibrio cholerae strain A1552

44.991

100

0.456
  pilB Vibrio campbellii strain DS40M4

45.672

96.791

0.442