Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ToN1_RS07545 Genome accession   NZ_CP059560
Coordinates   1634048..1635763 (+) Length   571 a.a.
NCBI ID   WP_169207740.1    Uniprot ID   -
Organism   Aromatoleum petrolei strain ToN1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629048..1640763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ToN1_RS07520 (ToN1_15430) - 1629407..1630180 (-) 774 WP_244860991.1 SprT family zinc-dependent metalloprotease -
  ToN1_RS07525 (ToN1_15440) - 1630177..1630929 (-) 753 WP_169207744.1 molybdopterin-binding protein -
  ToN1_RS07530 (ToN1_15450) - 1630933..1631703 (-) 771 WP_169207743.1 EI24 domain-containing protein -
  ToN1_RS07535 (ToN1_15460) ffh 1631716..1633074 (-) 1359 WP_169207742.1 signal recognition particle protein -
  ToN1_RS07540 (ToN1_15470) ccsA 1633117..1633944 (+) 828 WP_169207741.1 cytochrome c biogenesis protein CcsA -
  ToN1_RS07545 (ToN1_15480) pilB 1634048..1635763 (+) 1716 WP_169207740.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ToN1_RS07550 (ToN1_15490) pilC 1635780..1637012 (+) 1233 WP_169207739.1 type II secretion system F family protein Machinery gene
  ToN1_RS07555 (ToN1_15500) pilD 1637068..1637919 (+) 852 WP_169207738.1 A24 family peptidase Machinery gene
  ToN1_RS07560 (ToN1_15510) - 1638044..1638307 (+) 264 WP_169125945.1 zinc ribbon domain-containing protein -
  ToN1_RS07565 (ToN1_15520) - 1638323..1638949 (+) 627 WP_169207737.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62220.40 Da        Isoelectric Point: 5.3345

>NTDB_id=470617 ToN1_RS07545 WP_169207740.1 1634048..1635763(+) (pilB) [Aromatoleum petrolei strain ToN1]
MAANPQTALSGLARALIQHGRLSEADAAACTKSAANVPNGFILELAQRKLLTTKAVAWFAAETFGYPLLDISSVDRASIP
RDAIDRKLMAKHQVVALGKRQNRLTVATADPSNMRALDEIRFQTGMQVDLIVVEADRLARLAETLSESTEDTLKDLTGEE
FDMDLLQDQEATEQASEDASAEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLILKEKI
AARIKVISRLDISEKRIPQDGRMKLVLSKNKSIDFRVSSLPTLHGEKIVMRILDASSAMLGVDALGYELEQKKVLLDAIE
RPYGMILVTGPTGSGKTVSLYTCLNILNKPGVNISTAEDPAEINLAGINQVNVNDKAGLTFAASLRAFLRQDPDVIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVILITAQRLARRLCTCKQPVDIPVEALLDAG
FSGEDLDGSWRPYGPVGCERCKGSGYKGRLGIYQVMPISAEIAHIIMTNGNSMDIAAQAQREGVRDLRQSGLLKVKQGAT
SLEEVLATTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=470617 ToN1_RS07545 WP_169207740.1 1634048..1635763(+) (pilB) [Aromatoleum petrolei strain ToN1]
ATGGCAGCAAACCCCCAGACCGCCCTGAGCGGACTCGCGCGAGCACTGATCCAGCATGGCCGGCTCTCCGAAGCCGATGC
GGCCGCGTGCACGAAGAGCGCGGCAAACGTCCCGAACGGCTTCATCCTCGAACTTGCCCAGCGCAAGCTCCTGACCACCA
AGGCAGTTGCCTGGTTTGCTGCCGAAACCTTCGGCTACCCGCTGCTCGACATCTCGTCCGTCGACCGCGCCAGCATCCCC
CGCGATGCGATCGACCGCAAGCTGATGGCCAAGCACCAGGTCGTCGCCCTGGGGAAACGCCAGAACCGCCTCACCGTTGC
GACGGCCGACCCGTCCAACATGCGCGCACTCGACGAGATCCGCTTCCAGACCGGGATGCAGGTCGACCTCATCGTCGTCG
AAGCCGATCGCCTCGCCCGCCTTGCAGAAACCCTGTCGGAATCGACCGAGGACACGCTCAAGGACCTGACCGGCGAAGAG
TTCGACATGGACCTCCTGCAGGATCAGGAGGCCACGGAGCAGGCCAGCGAGGACGCCTCCGCAGAAGTCGATGACGCCCC
GGTCGTCAAGTTCATCCAGAAGGTCCTGATCGACGCGATCAACGAGGGCGCGTCCGACATCCACTTCGAACCGTACGAAA
AGTATTACCGCATCCGCGTCCGCACCGACGGCATCCTGCGCGAGATCGCGCAGCCGCCGCTGATCCTCAAGGAGAAGATC
GCCGCTCGCATCAAGGTCATTTCGCGTCTCGACATTTCGGAAAAACGCATCCCGCAGGACGGGCGCATGAAGCTCGTCCT
GTCCAAGAACAAGTCGATCGACTTCCGCGTATCGTCGCTGCCGACGCTGCATGGCGAAAAGATCGTCATGCGTATTCTCG
ATGCATCGTCGGCGATGCTGGGGGTCGACGCCCTCGGTTACGAGCTGGAGCAGAAAAAGGTGCTGCTCGATGCGATCGAG
CGCCCCTACGGAATGATCCTCGTAACGGGGCCGACCGGCTCTGGCAAGACCGTCTCGCTGTACACCTGCCTCAACATCCT
CAACAAGCCGGGCGTCAACATCTCGACGGCCGAGGACCCCGCGGAAATCAACCTGGCCGGCATCAACCAGGTCAACGTGA
ACGACAAGGCCGGCCTGACCTTCGCCGCATCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAA
ATCCGCGACCTCGAAACTGCCGAGATCTCCATCAAGGCGGCACAGACGGGCCACCTCGTGCTGTCGACGCTGCATACCAA
CGACGCGCCGACGACACTCGAGCGCCTGCGCAACATGGGCGTCGCGCCGTTCAACATCGCCTCCTCGGTGATCCTGATCA
CCGCCCAGCGCTTGGCGCGGCGCCTGTGCACGTGCAAGCAACCGGTCGATATCCCCGTGGAAGCCTTGCTCGATGCAGGC
TTCAGCGGCGAGGATCTCGACGGCAGCTGGCGCCCATACGGCCCCGTCGGTTGCGAACGATGCAAGGGCAGCGGCTACAA
GGGGCGACTGGGTATCTATCAGGTCATGCCCATTTCCGCGGAAATTGCCCATATCATCATGACCAACGGCAATTCGATGG
ATATTGCGGCACAGGCCCAACGCGAAGGCGTGCGCGACCTGCGCCAGTCCGGCCTGCTGAAAGTGAAACAGGGTGCGACC
TCGCTCGAGGAAGTGCTCGCCACCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.654

99.124

0.552
  pilB Acinetobacter baumannii D1279779

55.124

99.124

0.546
  pilF Neisseria gonorrhoeae MS11

53.439

99.299

0.531
  pilB Legionella pneumophila strain ERS1305867

52.787

100

0.531
  pilB Vibrio cholerae strain A1552

48.995

95.797

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

50.383

91.419

0.461
  pilB Vibrio campbellii strain DS40M4

49.318

89.842

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.866

92.995

0.38
  pilF Thermus thermophilus HB27

39.926

94.746

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

41.449

87.04

0.361