Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H2788_RS17145 Genome accession   NZ_CP059550
Coordinates   3597973..3599685 (+) Length   570 a.a.
NCBI ID   WP_347016649.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain E-022166     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3592973..3604685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2788_RS17110 (H2788_17015) nusA 3593863..3595347 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  H2788_RS17115 (H2788_17020) rimP 3595385..3595909 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  H2788_RS17135 (H2788_17040) secG 3596547..3596876 (-) 330 WP_306217788.1 preprotein translocase subunit SecG -
  H2788_RS17140 (H2788_17045) tpiA 3596889..3597683 (-) 795 WP_049064232.1 triose-phosphate isomerase -
  H2788_RS17145 (H2788_17050) pilB 3597973..3599685 (+) 1713 WP_347016649.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2788_RS17150 (H2788_17055) pilC 3599713..3600939 (+) 1227 WP_151740587.1 type II secretion system F family protein Machinery gene
  H2788_RS17155 (H2788_17060) pilD 3600939..3601799 (+) 861 WP_174735321.1 A24 family peptidase Machinery gene
  H2788_RS17160 (H2788_17065) coaE 3601801..3602397 (+) 597 WP_068935797.1 dephospho-CoA kinase -
  H2788_RS17165 (H2788_17070) - 3602394..3603308 (-) 915 WP_347016650.1 DMT family transporter -
  H2788_RS17170 (H2788_17075) rlmB 3603343..3604092 (-) 750 WP_019456777.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H2788_RS17175 (H2788_17080) - 3604195..3604521 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63489.87 Da        Isoelectric Point: 5.3337

>NTDB_id=470550 H2788_RS17145 WP_347016649.1 3597973..3599685(+) (pilB) [Acinetobacter seifertii strain E-022166]
MSALHTSPKFTGFFRRLVEEKHVSAATMQNALNAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLGQHFAEESNFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=470550 H2788_RS17145 WP_347016649.1 3597973..3599685(+) (pilB) [Acinetobacter seifertii strain E-022166]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAAATGCAGCAAAGAGAGCTAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTGGCTGAAACAATCTCTGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATTTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCGATCCGCTTTAACAGCAAACTGATGGTTGAGCCTGTCATTG
TCGAGCACCATAAACTAGAAAAAGTTTTAGGTCAGCATTTTGCAGAAGAAAGCAACTTTGACTTTAATGATGAAGAGTTT
GATCTTGATGTCAGTCTTGATGGCCCTACCACTCAAGAGGATGAAGAAGAAACGCCACAAGGTGATGAAGCCCCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGCGCTTCAGACTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTACGTTATCGAGTCGACGGTGTGTTACGTCAAATTGCTAATCCTCCTTTACAACTTGCGAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACATTGTTTGGTGAAAAGTTAGTTTTACGTATTCTTGACCCAT
CAAGTGCGATGCTGGGGATTGACGCATTAGGATACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAGGGCATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTCGGTGAAATTCGT
GACTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACCGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGAGTTCCCTCTTTTAATATTGCAACATCAGTAAATCTAGTGATTGCAC
AGCGTTTGGCACGACGTCTTTGCTCTCAATGTAAAATACCTACAGATGTTCCAAAACAAAGTTTATTGGAAATGGGCTTT
ACCGAGCAGGACTTAGCACATCCAGACTTTCGGGTTTTTCAACCTGTGGGTTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

97.895

100

0.979
  pilB Acinetobacter baylyi ADP1

80.175

100

0.802
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

92.982

0.479
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB Vibrio cholerae strain A1552

50.484

90.702

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365