Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M001_RS02125 Genome accession   NZ_CP059396
Coordinates   435417..437123 (-) Length   568 a.a.
NCBI ID   WP_021231278.1    Uniprot ID   -
Organism   Aeromonas veronii Hm21     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430417..442123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M001_RS02095 (M001_002095) mutT 430927..431331 (+) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -
  M001_RS02100 (M001_002100) yacG 431409..431603 (-) 195 WP_021231282.1 DNA gyrase inhibitor YacG -
  M001_RS02105 (M001_002105) zapD 431613..432335 (-) 723 WP_021231281.1 cell division protein ZapD -
  M001_RS02110 (M001_002110) coaE 432373..432987 (-) 615 WP_021231280.1 dephospho-CoA kinase -
  M001_RS02115 (M001_002115) pilD 433001..433873 (-) 873 WP_021231279.1 prepilin peptidase Machinery gene
  M001_RS02120 (M001_002120) pilC 433935..435176 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  M001_RS02125 (M001_002125) pilB 435417..437123 (-) 1707 WP_021231278.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  M001_RS02130 (M001_002130) - 437127..437591 (-) 465 WP_021231277.1 pilin -
  M001_RS02135 (M001_002135) nadC 437910..438770 (-) 861 WP_021231276.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M001_RS02140 (M001_002140) - 438774..439244 (-) 471 WP_021231275.1 retropepsin-like aspartic protease family protein -
  M001_RS02145 (M001_002145) ampD 439375..439950 (+) 576 WP_021231274.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M001_RS02150 (M001_002150) pdhR 440229..440993 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62549.87 Da        Isoelectric Point: 5.3170

>NTDB_id=469324 M001_RS02125 WP_021231278.1 435417..437123(-) (pilB) [Aeromonas veronii Hm21]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDDNSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=469324 M001_RS02125 WP_021231278.1 435417..437123(-) (pilB) [Aeromonas veronii Hm21]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCATTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAGCCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGTAAGGCGCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCATACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGGCTACATACCGAAGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAAATT
TCGGATCTCGAGGTCTCCGATGATAACAGCCGGCTCGACGAAAGCGTTAATACCACCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTTCGCTTTCGCATCGATGGCATTCTGCATGAAATCGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCGCGGCTCGATATTGCCGAGCGGCGGCTGCCACAGGATGGCCGTATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTTAATACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTTAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAACATCTCCACTGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCCATCAAGGCTGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCAGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTACCGGCTTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCAGAAGTAGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCGGCCGGGCTGCGACTGTTCAAACCAGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGTACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCA
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.415

100

0.565
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.562

95.246

0.539
  pilB Vibrio cholerae strain A1552

54.937

98.063

0.539
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

51.241

99.296

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

39.241

97.359

0.382
  pilF Thermus thermophilus HB27

40.53

92.958

0.377