Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HZY77_RS14340 Genome accession   NZ_CP059257
Coordinates   2910735..2912450 (+) Length   571 a.a.
NCBI ID   WP_296816171.1    Uniprot ID   A0A7L5YG76
Organism   MAG: Thiobacillus sp. isolate SSC5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2905735..2917450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZY77_RS14315 (HZY77_14165) rimM 2906298..2906804 (-) 507 WP_296816155.1 ribosome maturation factor RimM -
  HZY77_RS14320 (HZY77_14170) rpsP 2906806..2907057 (-) 252 WP_296816158.1 30S ribosomal protein S16 -
  HZY77_RS14325 (HZY77_14175) ffh 2907127..2908470 (-) 1344 WP_296816162.1 signal recognition particle protein -
  HZY77_RS14330 (HZY77_14180) ccsA 2908570..2909325 (+) 756 WP_296816165.1 cytochrome c biogenesis protein CcsA -
  HZY77_RS14335 (HZY77_14185) - 2909337..2910635 (+) 1299 WP_296816168.1 HlyC/CorC family transporter -
  HZY77_RS14340 (HZY77_14190) pilB 2910735..2912450 (+) 1716 WP_296816171.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HZY77_RS14345 (HZY77_14195) pilC 2912463..2913683 (+) 1221 WP_296816175.1 type II secretion system F family protein Machinery gene
  HZY77_RS14350 (HZY77_14200) pilD 2913683..2914534 (+) 852 WP_296816177.1 A24 family peptidase Machinery gene
  HZY77_RS14355 (HZY77_14205) coaE 2914534..2915142 (+) 609 WP_296816180.1 dephospho-CoA kinase -
  HZY77_RS14360 (HZY77_14210) zapD 2915195..2915950 (+) 756 WP_296816183.1 cell division protein ZapD -
  HZY77_RS14365 (HZY77_14215) - 2915947..2916141 (+) 195 WP_296816186.1 DNA gyrase inhibitor YacG -
  HZY77_RS14370 (HZY77_14220) rpiA 2916145..2916800 (-) 656 Protein_2841 ribose-5-phosphate isomerase RpiA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62531.59 Da        Isoelectric Point: 4.9287

>NTDB_id=468185 HZY77_RS14340 WP_296816171.1 2910735..2912450(+) (pilB) [MAG: Thiobacillus sp. isolate SSC5]
MAAVTNTNNLTGLARAMVNHGWLSEDDAEGVWKRASQSGDSFVTELIKGRRFKSDQVAEFAAASFGFPYLDLNAMDHESL
PQGLLDPKLVQKRRVLALYQRGNKLYVATSDPTHLQALDEVKFQIGQAVEPVVVEDDKLGKLIEKMGEANDNTMAVLNAE
DLNLDFADEESAAAQQDTGGIEIDDAPVVRYLQKIMLDAINIGASDIHFEPYEKFYRIRYRRDGVLSEVAQPPMAIKDKV
ASRIKVLSRLDISEKRVPQDGRMKMVLSKNRAIDFRVSTLPTLYGEKIVMRILDPTSAQLGIEALGYEPWQKELLLNAVQ
RPYGMVLVTGPTGSGKTVSLYTCLNILNKPDVNISTAEDPAEIQLPGINQVNVNDKAGLTFSAALKSFLRQDPDVIMVGE
IRDLETADIAIKAAQTGHMVMSTLHTNDAPGTLTRLLNMGVAPFNVASSVILITAQRLGRKLCSCKQPADIPQEALLAAG
FTEEQLDGSWKPYKPVGCDICGGTGYKGRVGIYQVMPITDEMTRIILKGGDESDIADQARREGVLDLRQAGLLKVKSGLT
SLEEVEAVTNL

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=468185 HZY77_RS14340 WP_296816171.1 2910735..2912450(+) (pilB) [MAG: Thiobacillus sp. isolate SSC5]
ATGGCTGCTGTGACAAACACCAACAATTTAACCGGGCTGGCGCGTGCCATGGTCAACCATGGCTGGCTGTCCGAGGACGA
CGCCGAGGGAGTGTGGAAACGCGCCAGCCAGTCGGGCGATTCGTTCGTGACCGAATTGATCAAAGGGCGGCGGTTCAAGT
CTGATCAAGTTGCCGAGTTTGCGGCTGCCTCGTTCGGTTTTCCCTACCTGGACCTGAATGCCATGGATCATGAGAGCCTG
CCGCAAGGGCTGCTCGATCCGAAACTGGTGCAGAAACGGCGGGTGCTCGCCTTGTATCAACGGGGCAACAAGCTGTATGT
GGCGACGTCAGACCCTACGCATCTGCAGGCGCTGGACGAGGTGAAATTCCAGATCGGCCAGGCGGTGGAGCCCGTGGTGG
TGGAGGACGACAAGCTCGGCAAGCTCATCGAGAAGATGGGTGAAGCGAACGACAACACGATGGCTGTGTTGAATGCCGAA
GACCTCAATCTGGATTTCGCCGACGAGGAAAGTGCCGCTGCCCAGCAGGACACGGGCGGCATCGAAATCGACGATGCGCC
CGTGGTGCGCTATCTGCAGAAGATCATGCTCGACGCCATCAATATCGGCGCATCCGACATCCATTTCGAGCCGTACGAGA
AGTTCTACCGCATCCGCTACCGGCGTGACGGCGTCTTGTCCGAAGTGGCCCAGCCGCCAATGGCGATCAAGGATAAAGTG
GCCTCGCGCATCAAGGTGTTGTCGCGGCTGGATATTTCCGAAAAGCGCGTGCCGCAGGATGGCCGCATGAAAATGGTGCT
GTCGAAGAACCGCGCCATCGACTTCCGGGTCAGCACCTTGCCCACGCTGTATGGCGAAAAGATCGTCATGCGTATTCTCG
ATCCCACTAGCGCGCAGTTGGGGATCGAGGCGCTCGGCTACGAGCCGTGGCAGAAGGAACTGCTGCTGAATGCGGTGCAA
CGCCCCTATGGCATGGTGCTGGTGACCGGTCCGACCGGTTCCGGCAAGACGGTGTCGCTCTATACCTGCCTGAATATCCT
CAACAAGCCCGACGTCAACATCTCGACGGCTGAAGACCCGGCGGAAATCCAGTTGCCCGGAATCAACCAGGTCAACGTCA
ACGACAAGGCCGGACTGACGTTCTCGGCGGCGTTGAAATCCTTCCTGCGGCAGGATCCAGACGTCATCATGGTCGGTGAG
ATCCGCGACCTGGAAACCGCCGATATCGCCATCAAGGCCGCGCAAACCGGCCATATGGTGATGTCCACTCTGCACACCAA
CGATGCGCCCGGAACCCTCACTCGTCTGTTGAACATGGGCGTGGCGCCGTTCAACGTGGCCTCCTCGGTGATCCTGATTA
CCGCGCAACGGCTAGGGCGGAAGTTGTGCAGCTGCAAACAGCCTGCCGATATTCCACAGGAAGCGCTGCTGGCGGCAGGC
TTTACCGAGGAGCAGCTCGACGGCAGCTGGAAACCTTACAAGCCGGTGGGGTGCGATATCTGTGGCGGCACAGGCTATAA
AGGGCGTGTAGGCATCTACCAGGTCATGCCGATCACAGATGAAATGACTCGCATCATCCTCAAGGGCGGGGACGAATCCG
ATATTGCCGACCAGGCACGACGCGAAGGCGTGCTCGACCTGCGGCAGGCAGGTCTGTTGAAAGTGAAATCCGGTCTCACC
TCGCTGGAAGAGGTCGAGGCCGTTACCAATCTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(18-148)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L5YG76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.196

100

0.543
  pilF Neisseria gonorrhoeae MS11

54.77

99.124

0.543
  pilB Acinetobacter baylyi ADP1

52.972

100

0.531
  pilB Legionella pneumophila strain ERS1305867

52.34

100

0.529
  pilB Vibrio cholerae strain A1552

47.527

99.124

0.471
  pilB Vibrio campbellii strain DS40M4

46.935

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.831

99.475

0.466
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.716

98.774

0.392
  pilF Thermus thermophilus HB27

38.516

99.124

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

41.071

88.266

0.363