Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H0S56_RS01330 Genome accession   NZ_CP059081
Coordinates   299524..301233 (-) Length   569 a.a.
NCBI ID   WP_195725487.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain 12CE1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 294524..306233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S56_RS01300 (H0S56_01300) - 294643..294966 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  H0S56_RS01305 (H0S56_01305) rlmB 295101..295850 (+) 750 WP_005094401.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H0S56_RS01310 (H0S56_01310) - 295857..296780 (+) 924 WP_005094400.1 DMT family transporter -
  H0S56_RS01315 (H0S56_01315) coaE 296802..297401 (-) 600 WP_195725484.1 dephospho-CoA kinase -
  H0S56_RS14390 (H0S56_01320) pilD 297403..298263 (-) 861 WP_195725485.1 A24 family peptidase Machinery gene
  H0S56_RS01325 (H0S56_01325) pilC 298263..299492 (-) 1230 WP_195725486.1 type II secretion system F family protein Machinery gene
  H0S56_RS01330 (H0S56_01330) pilB 299524..301233 (-) 1710 WP_195725487.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H0S56_RS01335 (H0S56_01335) tpiA 301511..302302 (+) 792 WP_195725488.1 triose-phosphate isomerase -
  H0S56_RS01340 (H0S56_01340) secG 302316..302645 (+) 330 WP_005248398.1 preprotein translocase subunit SecG -
  H0S56_RS01370 (H0S56_01370) rimP 303603..304127 (+) 525 WP_004281103.1 ribosome maturation factor RimP -
  H0S56_RS01375 (H0S56_01375) nusA 304162..305646 (+) 1485 WP_004281105.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63528.70 Da        Isoelectric Point: 4.8316

>NTDB_id=467115 H0S56_RS01330 WP_195725487.1 299524..301233(-) (pilB) [Acinetobacter lwoffii strain 12CE1]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLSPTVIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKHRILPIFKNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDVDTSDPNKEDEESNKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=467115 H0S56_RS01330 WP_195725487.1 299524..301233(-) (pilB) [Acinetobacter lwoffii strain 12CE1]
ATGACAGCATTACAGGGGTCGCCGAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCGTTGGCGCATGCCAAACAAGAAAAAATCGATATTGTCGCGGAGCTGATTAACCAGCAACAGCTTTCTC
CGACCGTCATTGCTGAAACGATTTCAGTTGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCCCAAATT
TTGCGGGATGCGATTGATGAAAAGCTGATTACCAAGCATCGCATCCTGCCGATTTTTAAAAATTCCAGTATTTTATATGT
TGCGACCAGCAACCCGACCAATATCGAAGCAATTGATGCAGTCCGTTTTTCGACCAAACTTAACATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAACAGAATTTTACCGAAGAAAGTACCTTTGAATTCGATGAAGATTTCGAC
CTGGATGTTGATGTCGACACCTCAGATCCGAATAAAGAAGATGAAGAAAGCAATAAAGGTGATGAAGCGCCGATTGTCAA
ATATATTAATAAATTGCTGATTGATGCGATCCGTATGGGGGCTTCAGACTTACACTTTGAACCTTATGAAAAAATCTATC
GGGTACGTTACCGGGTCGATGGGGTGCTACGTCAGATTGCAACCCCGCCCTTACAGCTGGCAAATCGCCTGTCTTCGCGT
CTTAAGGTTATGTCGCAAATGGACATTTCGGAAAAGCGTGTACCTCAAGATGGTCGTATTAAGTTAAAACTTTCTAAGAA
TAAAGCCATTGATTTCCGTGTTAACTCTCTCCCTACCCTGTTTGGCGAGAAGCTGGTACTGCGTATTCTGGATCCATCCA
GCGCGATGCTGGGGATTGATGCTCTGGGCTATGAACCGGAACAAAAAGAACTGTTTATGCAGGCACTGGACAAACCGCAA
GGGATGCTGCTGATCACCGGTCCGACCGGTTCCGGTAAAACAGTTTCTCTTTATACCGGTCTGAATATTTTAAACCGTGA
AGATACCAATATTTCCACAGCGGAAGATCCGGTCGAGATCAACCTACAAGGGATTAACCAGGTCAACGTCAACAATAAAG
TCGGCCTGACCTTCTCTGCTGCCTTGAAGTCCTTCTTACGTCAAGATCCGGATATCGTGATGGTCGGTGAGATTCGGGAT
CTGGAAACTGCAGAGATTGCGATTAAAGCGGCCCAGACCGGCCATATGGTGATGTCGACCTTACATACCAATAGTGCGCC
TGAGACTTTGACCCGTTTACGCAATATGGGGGTGCCTTCTTTCAATATTGCCACTTCAGTCAACCTGGTCATTGCACAAC
GGTTGGCGCGTCGCCTGTGTTCACAGTGCAAAAAACCTGCCGATATTCCGAAACAAAGCTTATTGGAAATGGGCTTTACC
GAAACTGATCTGCAGCAACCAGAATTCCAAATCTATGAACCAGTAGGTTGCAATGAATGTCGTGAAGGCTATAAAGGTCG
TGTGGGTATTTATGAAGTCATGAAAGTAACACCCGAAATTTCCAGAATTATTATGGAAGACGGCAATGCACTTGAAATTG
CCGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.053

100

0.812
  pilB Acinetobacter baylyi ADP1

77.193

100

0.773
  pilB Legionella pneumophila strain ERS1305867

51.767

99.473

0.515
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio campbellii strain DS40M4

49.104

98.067

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.192

97.891

0.482
  pilB Vibrio cholerae strain A1552

51.064

90.861

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

35.073

100

0.378
  pilF Thermus thermophilus HB27

37.102

99.473

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.544

100

0.367