Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H0S58_RS12215 Genome accession   NZ_CP059079
Coordinates   2616663..2618375 (+) Length   570 a.a.
NCBI ID   WP_053578933.1    Uniprot ID   A0A1C4GWE4
Organism   Acinetobacter sp. TTH0-4 strain 1BD1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2611663..2623375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S58_RS12175 (H0S58_12180) nusA 2612416..2613900 (-) 1485 WP_053578929.1 transcription termination factor NusA -
  H0S58_RS12180 (H0S58_12185) rimP 2613940..2614464 (-) 525 WP_053578930.1 ribosome maturation factor RimP -
  H0S58_RS12205 (H0S58_12210) secG 2615248..2615577 (-) 330 WP_053578931.1 preprotein translocase subunit SecG -
  H0S58_RS12210 (H0S58_12215) tpiA 2615591..2616382 (-) 792 WP_193889706.1 triose-phosphate isomerase -
  H0S58_RS12215 (H0S58_12220) pilB 2616663..2618375 (+) 1713 WP_053578933.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H0S58_RS12220 (H0S58_12225) pilC 2618405..2619631 (+) 1227 WP_195726649.1 type II secretion system F family protein Machinery gene
  H0S58_RS12225 (H0S58_12230) pilD 2619631..2620491 (+) 861 WP_195726650.1 prepilin peptidase Machinery gene
  H0S58_RS12230 (H0S58_12235) coaE 2620505..2621095 (+) 591 WP_195726651.1 dephospho-CoA kinase -
  H0S58_RS12235 (H0S58_12240) - 2621112..2622032 (-) 921 WP_195726652.1 DMT family transporter -
  H0S58_RS12240 (H0S58_12245) rlmB 2622070..2622819 (-) 750 WP_092720517.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H0S58_RS12245 (H0S58_12250) - 2622951..2623274 (-) 324 WP_053578939.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63667.34 Da        Isoelectric Point: 5.0243

>NTDB_id=467055 H0S58_RS12215 WP_053578933.1 2616663..2618375(+) (pilB) [Acinetobacter sp. TTH0-4 strain 1BD1]
MAALQSSPKFTGFIRRLVEDEIVSAENMQNAILSAKKAHQDIVPYLIDQLKIPALQIAEKISQEFGEPLFDLSIYDPAQI
IREGIDEKIITKHRILPIFKRGNLLYIATSNPTNMDAMDAIRFSSKMNVEAIIVEYPKLQKLIEQNFAEADTFEFSDDDI
DLNLEQEDISQKEDEDDDQKADEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLATRLAS
RLKVMSQMDISEKRLPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNAKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPANIPEPSLLEMGF
TEADLKHPDFHVYQPAGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASLKSGFNNLRRSGLMKVMQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=467055 H0S58_RS12215 WP_053578933.1 2616663..2618375(+) (pilB) [Acinetobacter sp. TTH0-4 strain 1BD1]
ATGGCAGCACTACAGAGTTCACCAAAATTTACGGGATTTATTCGACGTTTAGTTGAGGATGAGATCGTTTCTGCTGAAAA
CATGCAGAATGCAATATTAAGTGCAAAAAAAGCCCATCAAGATATTGTTCCTTATTTAATTGATCAGTTAAAAATACCGG
CCTTACAAATTGCAGAAAAAATTTCTCAGGAATTTGGTGAACCGCTATTCGACTTGAGCATTTATGATCCAGCCCAGATT
ATTCGTGAAGGAATTGATGAGAAAATTATAACCAAACATCGCATTCTTCCGATTTTTAAACGCGGCAATTTACTTTATAT
CGCCACTAGCAATCCCACCAATATGGATGCCATGGACGCGATTCGTTTTAGCAGTAAAATGAATGTTGAAGCCATTATTG
TTGAATACCCAAAATTACAAAAACTCATTGAGCAAAATTTTGCTGAAGCAGATACATTTGAATTTTCAGATGATGATATC
GATCTTAATCTTGAACAGGAGGATATCAGCCAAAAAGAGGATGAAGATGATGACCAGAAGGCAGATGAAGCACCGATTGT
AAAATATATTAATAAGTTACTGATTGATGCAATTCGTATGGGTGCGTCAGATTTACATTTTGAACCGTATGAAAAAATGT
ATCGTGTGCGTTATCGTGTGGATGGTGTATTAAGACAGATAGCCAATCCACCGCTGCAACTTGCTACACGGTTGGCTTCG
CGTTTAAAAGTCATGTCGCAAATGGATATTTCAGAAAAACGCCTACCTCAAGATGGGCGAATTAAACTTAAACTTTCGAA
AAGTAAAGCCATTGATTTTCGTGTGAATTCACTCCCTACTCTATTTGGTGAAAAATTAGTTCTGCGTATTCTCGATCCTT
CAAGTGCCATGCTTGGAATTGACGCTTTAGGTTATGAACCAGAGCAAAAAGAATTGTTCATGCAAGCACTAGATAAACCT
CAAGGCATGTTATTGATTACAGGACCTACGGGTTCAGGTAAAACGGTATCTCTATATACAGGTTTGAATATTCTGAACCG
TGAAGATACCAATATTTCAACTGCCGAAGATCCTGTGGAAATTAACTTGGAAGGGATTAATCAGGTTAATGTAAATGCAA
AAGTGGGATTAACATTCTCTGCTGCACTTAAATCTTTCTTGCGACAAGATCCTGATATTGTCATGGTCGGTGAGATTCGA
GATTTAGAAACGGCTGAAATTGCAATTAAAGCAGCGCAAACAGGTCATATGGTCATGTCCACATTACATACGAACAGTGC
ACCTGAAACCTTGACCCGACTTAGAAATATGGGTGTACCTTCCTTCAATATTGCAACCTCGGTAAACTTGGTGATTGCAC
AGCGTCTAGCTCGACGCTTATGTCCACAATGTAAAGTACCCGCGAATATTCCAGAACCTAGCTTATTAGAAATGGGTTTT
ACTGAAGCAGACCTCAAACATCCAGATTTCCATGTTTATCAACCAGCAGGCTGCTCAGAATGCCGAGAAGGCTACAAAGG
CCGTGTTGGGATTTATGAAGTAATGAAAGTAACACCTGAAATTTCAAAAATTATTATGGAAGATGGCAATGCACTTGAAA
TTGCTGCTGCATCTTTAAAATCGGGGTTTAATAATTTGCGTCGTTCAGGATTAATGAAAGTCATGCAAGGAATCACGTCA
CTTCAAGAAGTGAATCGTGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4GWE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.474

100

0.795
  pilB Acinetobacter baylyi ADP1

76.316

100

0.763
  pilB Legionella pneumophila strain ERS1305867

53.731

94.035

0.505
  pilB Vibrio cholerae strain A1552

49.912

99.474

0.496
  pilF Neisseria gonorrhoeae MS11

48.932

98.596

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

51.644

90.702

0.468
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.249

100

0.372
  pilF Thermus thermophilus HB27

37.117

97.368

0.361