Detailed information NTG0466817

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HZ322_RS08605
• Genome accession: NZ_CP059049
• Coordinates: 1824625..1825272 (-)
• Length: 215 a.a.
• NCBI ID: WP_017864913.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain N8
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1819625..1830272

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZ322_RS08580 (HZ322_08580)	atpF	1819694..1820200 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
HZ322_RS08585 (HZ322_08585)	atpB	1820215..1820928 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
HZ322_RS08590 (HZ322_08590)	-	1820973..1821188 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
HZ322_RS08595 (HZ322_08595)	-	1821373..1822149 (-)	777	WP_017864911.1	alpha/beta hydrolase family protein	-
HZ322_RS08600 (HZ322_08600)	comEC	1822488..1824644 (-)	2157	WP_017864912.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HZ322_RS08605 (HZ322_08605)	comEA	1824625..1825272 (-)	648	WP_017864913.1	ComEA family DNA-binding protein	Machinery gene
HZ322_RS08610 (HZ322_08610)	-	1825332..1826693 (-)	1362	WP_017864914.1	ABC transporter permease	-
HZ322_RS08615 (HZ322_08615)	-	1826690..1827622 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
HZ322_RS08620 (HZ322_08620)	-	1827728..1828126 (-)	399	WP_004255236.1	hypothetical protein	-
HZ322_RS08625 (HZ322_08625)	-	1828230..1828793 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
HZ322_RS08630 (HZ322_08630)	-	1828966..1830144 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23598.73 Da
• Isoelectric Point: 5.0013

>NTDB_id=466817 HZ322_RS08605 WP_017864913.1 1824625..1825272(-) (comEA) [Lactococcus lactis strain N8]
MDKILEKVKEYWKMIVLVVCGLIAGGIFHVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=466817 HZ322_RS08605 WP_017864913.1 1824625..1825272(-) (comEA) [Lactococcus lactis strain N8]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTCACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	38.696	100	0.414
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367