Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   HZ322_RS08600 Genome accession   NZ_CP059049
Coordinates   1822488..1824644 (-) Length   718 a.a.
NCBI ID   WP_017864912.1    Uniprot ID   A0AAE4T0Y4
Organism   Lactococcus lactis strain N8     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1817488..1829644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ322_RS08575 (HZ322_08575) - 1819165..1819692 (-) 528 WP_012898339.1 F0F1 ATP synthase subunit delta -
  HZ322_RS08580 (HZ322_08580) atpF 1819694..1820200 (-) 507 WP_010906128.1 F0F1 ATP synthase subunit B -
  HZ322_RS08585 (HZ322_08585) atpB 1820215..1820928 (-) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  HZ322_RS08590 (HZ322_08590) - 1820973..1821188 (-) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  HZ322_RS08595 (HZ322_08595) - 1821373..1822149 (-) 777 WP_017864911.1 alpha/beta hydrolase family protein -
  HZ322_RS08600 (HZ322_08600) comEC 1822488..1824644 (-) 2157 WP_017864912.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HZ322_RS08605 (HZ322_08605) comEA 1824625..1825272 (-) 648 WP_017864913.1 ComEA family DNA-binding protein Machinery gene
  HZ322_RS08610 (HZ322_08610) - 1825332..1826693 (-) 1362 WP_017864914.1 ABC transporter permease -
  HZ322_RS08615 (HZ322_08615) - 1826690..1827622 (-) 933 WP_004255238.1 ABC transporter ATP-binding protein -
  HZ322_RS08620 (HZ322_08620) - 1827728..1828126 (-) 399 WP_004255236.1 hypothetical protein -
  HZ322_RS08625 (HZ322_08625) - 1828230..1828793 (-) 564 WP_004255233.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 718 a.a.        Molecular weight: 82234.53 Da        Isoelectric Point: 9.5820

>NTDB_id=466816 HZ322_RS08600 WP_017864912.1 1822488..1824644(-) (comEC) [Lactococcus lactis strain N8]
MSCVLNKIPLIFLAYLSVILYFLIFNFNLICLILFIFSSLLALYRKYYLVIPFLLVMGSFFLVVKMDTIKNSENQPTTLS
KITMIPDTIQVNGDLLSFQGKERGQNYQVYETLKSKKEQKFYQNLSQNCQLSFTGNLQIPEKQRNFNGFDNQKYLASQNI
YRQITIDKINKIVLNDTFDLHVLRRKAIVWSQTHFPKPMNDYMTGLLFGFLSKDFNQIGDIYSSLGIIHLFALSGMQVNF
FIDWFRRIILRLGITREKLNIWQIPFSIFYAFMTGFSVSVLRALFQKNIRLNPLDNLAVTTFLLMIVSPKFLLTIGGQLT
LFYAFVISMINHKFSELKGIRKLLTESSVISLSVLPLLILDFHIFQPFSILLTIGFGFLFDVILLPLLLGTFLLSLIGYN
FNINHIFQILEWLIHEVDLPLHYPLVLGNPRPIELLILFFLIGLLIDNLFKKGRQIIFAGLIVCSFFICKNPVYPSITAV
DIGQGDSIFLQDKFNKKTILIDTGGRLALPQESWQKPQSQTNAEKTLIPYLESVGVSQIDQLILTHTDADHVGDFLNLAD
KIKIKEIWVSPGELTNNHFVEKLKKANIAIHISKVGDKIPIFDSALQVLSNGYTGKGDNNDSIVTYGNFYHTKFLFTGDL
EQEGEKELLKNYPKLKVDVLKVGHHGSKTSSNPDFIKEINPKLALISVGEKNRYGHPNQETLETLKKNQIRILRTDQK

Nucleotide


Download         Length: 2157 bp        

>NTDB_id=466816 HZ322_RS08600 WP_017864912.1 1822488..1824644(-) (comEC) [Lactococcus lactis strain N8]
ATGAGTTGTGTTTTGAATAAAATTCCTTTGATATTTTTAGCTTATCTTTCTGTTATCTTGTATTTTTTAATCTTCAATTT
TAATTTAATTTGTCTCATTTTGTTCATTTTTTCAAGTTTACTTGCCTTATATAGAAAATATTATTTAGTCATCCCATTTT
TATTAGTTATGGGAAGTTTTTTCTTAGTAGTAAAAATGGATACGATTAAGAATAGTGAAAATCAGCCAACCACTCTTTCC
AAAATTACAATGATCCCCGACACTATCCAAGTTAATGGTGATTTATTAAGTTTTCAAGGAAAAGAAAGAGGCCAAAATTA
TCAAGTTTACGAGACTCTAAAAAGTAAAAAAGAACAAAAATTTTATCAAAATCTAAGTCAAAATTGTCAGCTGAGTTTCA
CAGGAAACCTTCAAATTCCAGAAAAACAAAGAAATTTTAATGGATTCGATAATCAAAAGTATTTAGCGAGTCAGAATATT
TATCGGCAAATTACGATTGATAAAATTAATAAAATAGTTTTAAACGACACTTTTGACCTCCATGTTTTAAGACGAAAAGC
AATTGTCTGGTCACAAACTCATTTTCCAAAGCCAATGAATGATTATATGACTGGCTTACTTTTTGGCTTTTTAAGTAAAG
ATTTTAATCAAATAGGAGACATTTACAGTTCATTAGGAATTATTCATCTTTTTGCCTTATCGGGAATGCAAGTTAATTTT
TTCATTGATTGGTTTCGTAGAATTATTCTTCGACTAGGAATAACGAGAGAAAAATTAAATATTTGGCAAATTCCCTTTTC
AATTTTTTATGCATTTATGACAGGATTTTCGGTTTCAGTATTACGAGCTTTATTTCAAAAAAATATTCGTCTAAATCCTT
TAGATAACCTAGCAGTGACAACCTTTCTTCTGATGATTGTCAGTCCTAAATTTTTACTGACAATAGGAGGACAACTAACT
TTATTTTATGCTTTTGTCATTTCAATGATTAATCATAAATTTTCAGAACTAAAAGGAATAAGAAAATTACTGACAGAATC
ATCAGTGATTTCTTTGAGTGTTTTACCTTTACTCATTTTGGATTTTCATATCTTTCAGCCTTTTTCAATTTTACTGACGA
TTGGCTTTGGCTTTTTATTTGATGTAATCTTATTGCCTTTACTATTAGGAACTTTTCTCTTGTCTCTAATTGGCTATAAT
TTTAATATAAATCATATTTTTCAAATTCTAGAATGGCTAATTCATGAGGTTGATTTACCCTTACATTATCCTTTGGTTTT
AGGGAATCCCCGGCCAATTGAATTATTAATCTTATTTTTCCTAATCGGTTTGTTAATTGATAATCTCTTTAAGAAAGGAA
GACAAATAATTTTTGCAGGCCTAATTGTTTGTAGTTTTTTTATTTGTAAAAATCCAGTTTACCCCAGTATCACAGCAGTT
GATATTGGCCAAGGAGATAGTATTTTTTTGCAAGATAAATTTAATAAAAAAACAATTTTGATTGATACAGGCGGACGATT
AGCCCTTCCGCAAGAAAGTTGGCAAAAACCTCAAAGTCAAACAAATGCCGAAAAAACACTCATTCCATATCTTGAATCTG
TTGGAGTAAGTCAAATCGACCAGCTCATTTTGACGCATACGGACGCTGACCATGTTGGAGATTTTTTGAATTTAGCTGAT
AAAATAAAAATAAAAGAAATTTGGGTTAGCCCCGGTGAACTGACAAATAATCATTTTGTAGAAAAATTGAAGAAAGCAAA
TATTGCGATTCACATCAGTAAAGTTGGAGATAAAATTCCAATCTTTGATAGTGCTTTACAAGTTTTATCAAATGGTTATA
CAGGAAAAGGGGATAATAATGATTCTATCGTGACTTATGGAAATTTTTATCATACAAAATTTCTATTTACAGGGGATTTA
GAACAAGAAGGAGAAAAAGAACTGCTCAAGAATTATCCTAAATTGAAAGTTGATGTCTTAAAAGTAGGTCATCATGGCAG
TAAAACAAGTTCAAACCCTGACTTTATTAAAGAAATTAATCCCAAACTTGCCCTCATTTCGGTTGGAGAGAAAAATCGAT
ATGGACATCCCAATCAAGAAACTTTAGAAACATTAAAAAAAAACCAAATCAGAATACTGAGAACCGATCAAAAGTGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(206-446)

Domain of unknown function (DUF4131) (DUF4131)

(27-171)

Metallo-beta-lactamase superfamily (Lactamase_B)

(483-689)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

77.159

100

0.772
  comEC/celB Streptococcus mitis SK321

44.766

100

0.453
  comEC/celB Streptococcus mitis NCTC 12261

44.613

100

0.45
  comEC/celB Streptococcus pneumoniae TIGR4

43.862

100

0.443
  comEC/celB Streptococcus pneumoniae Rx1

43.448

100

0.439
  comEC/celB Streptococcus pneumoniae D39

43.448

100

0.439
  comEC/celB Streptococcus pneumoniae R6

43.448

100

0.439