Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HXV88_RS02070 Genome accession   NZ_CP058912
Coordinates   420090..421796 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain JC529     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415090..426796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXV88_RS02040 (HXV88_02040) mutT 415600..416004 (+) 405 WP_179903026.1 8-oxo-dGTP diphosphatase MutT -
  HXV88_RS02045 (HXV88_02045) yacG 416082..416276 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  HXV88_RS02050 (HXV88_02050) zapD 416286..417008 (-) 723 WP_179903027.1 cell division protein ZapD -
  HXV88_RS02055 (HXV88_02055) coaE 417046..417660 (-) 615 WP_179903028.1 dephospho-CoA kinase -
  HXV88_RS02060 (HXV88_02060) pilD 417674..418546 (-) 873 WP_179903029.1 A24 family peptidase Machinery gene
  HXV88_RS02065 (HXV88_02065) pilC 418608..419849 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  HXV88_RS02070 (HXV88_02070) pilB 420090..421796 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HXV88_RS02075 (HXV88_02075) - 421800..422228 (-) 429 WP_115544754.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HXV88_RS02080 (HXV88_02080) nadC 422544..423407 (-) 864 WP_179903030.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HXV88_RS02085 (HXV88_02085) - 423411..423881 (-) 471 WP_005336129.1 TIGR02281 family clan AA aspartic protease -
  HXV88_RS02090 (HXV88_02090) ampD 424012..424587 (+) 576 WP_179903031.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HXV88_RS02095 (HXV88_02095) pdhR 424866..425630 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=465950 HXV88_RS02070 WP_005336134.1 420090..421796(-) (pilB) [Aeromonas veronii strain JC529]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=465950 HXV88_RS02070 WP_005336134.1 420090..421796(-) (pilB) [Aeromonas veronii strain JC529]
ATGACCTCTAGCCCGAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCGCTGATAAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAAAACGAGATCCTTGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTTGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAGCACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGTCTTCATACCGAAGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGATGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAAAACAGCCGGCTGGACGAGAGCGTCAATACCGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTACACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGACGGCATTCTGCATGAAATCGCTACACCGCCGGTCAACCTCTCCAACCGCTTTTCCGCCCGC
CTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGTTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGAGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGACAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCTTGCGCTCCTTCTTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCAGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAACTGGCGGCCGGGCTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATATACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATTATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAAGTCAACCGGGTCACCACCAACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377